| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,968,268 | (C)5→6 | coding (167/2247 nt) | ptsP ← | phosphoenolpyruvate‑protein phosphotransferase PtsP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,968,263 | 1 | . | C | 97.7% | 148.9 / ‑3.2 | 44 | coding (172/2247 nt) | ptsP | phosphoenolpyruvate‑protein phosphotransferase PtsP |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (21/22); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
TCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAG‑CCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCGTGGTG > NC_000913/2968124‑2968391 | tcttTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCATGG‑TTTTTTCAg < 2:398466/140‑1 (MQ=255) tACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAG‑ccccc < 2:221027/140‑1 (MQ=255) ggggATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTc > 2:31730/1‑140 (MQ=255) gggATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCg > 1:508355/1‑140 (MQ=255) tGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCAt < 1:31730/140‑1 (MQ=255) tATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCa < 1:541277/140‑1 (MQ=255) tttGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAgg > 2:32531/1‑140 (MQ=255) tGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGgta > 2:664011/1‑140 (MQ=255) aaGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGgtagt > 1:261719/1‑140 (MQ=255) aaGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAG‑ccccccgggt < 1:168012/126‑6 (MQ=255) aaGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAG‑CCCCCcgggt > 2:168012/1‑124 (MQ=255) aaGCTGGGGTGCTTTTGCGCATCTGCAAGGATTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGgtagt < 2:508355/140‑1 (MQ=255) aGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGgtagta > 1:809248/1‑140 (MQ=255) gggTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCAt < 2:475499/126‑1 (MQ=255) gggTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCAt > 1:475499/1‑126 (MQ=255) gggTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTaacaac < 2:809248/140‑1 (MQ=255) gggTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTaacaac > 2:236350/1‑140 (MQ=255) ggTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACg < 1:668164/140‑1 (MQ=255) cTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGa < 2:721992/140‑1 (MQ=255) gcgcATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATg < 1:664011/140‑1 (MQ=255) gcgcATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATg < 2:261719/140‑1 (MQ=255) cgcATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACg < 2:183759/130‑1 (MQ=255) cgcATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACg > 1:183759/1‑130 (MQ=255) aTCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCg < 1:150417/140‑1 (MQ=255) tATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGAcc > 2:617011/1‑140 (MQ=255) tATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGAcc > 1:30326/1‑140 (MQ=255) gTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACa < 2:744114/140‑1 (MQ=255) gTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACa < 1:604155/140‑1 (MQ=255) ccGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGAc < 1:748840/140‑1 (MQ=255) tGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATc < 2:691412/140‑1 (MQ=255) cAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCAt < 1:166174/140‑1 (MQ=255) aGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCaa > 1:103043/1‑140 (MQ=255) aGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCaa > 1:444142/1‑140 (MQ=255) atcaAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAAcc < 1:32306/140‑1 (MQ=255) caAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCa > 2:799808/1‑140 (MQ=255) tGCGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCAtt > 1:43310/1‑140 (MQ=255) cGACCGCGTGG‑TTTTTTCAGCCCCCCGGGTCACCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCa > 1:262660/1‑140 (MQ=255) ccGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGcc < 2:540089/130‑1 (MQ=255) ccGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGcc > 1:540089/1‑130 (MQ=255) ccGCGTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGc > 1:374179/1‑140 (MQ=255) cgcgTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAcgcg < 1:474332/140‑1 (MQ=255) cgcgTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCg < 2:374179/140‑1 (MQ=255) gcgTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCgt > 1:556195/1‑140 (MQ=255) cgTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCgtg > 2:124548/1‑140 (MQ=255) cgTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCgtg > 2:737047/1‑140 (MQ=255) cgTGG‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCgtg < 2:30326/140‑1 (MQ=255) gg‑TTTTTTCAGCCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCgtggtg < 1:124548/140‑1 (MQ=255) | TCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTTCAG‑CCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCGTGGTG > NC_000913/2968124‑2968391 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |