Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,999,755	(C)6→7	coding (137/756 nt)	ygeR ←	LysM domain‑containing putative peptidase lipoprotein YgeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,999,749	1	.	C	100.0%	188.9 / NA	54	D48G (GAT→GGT)	ygeR	LysM domain‑containing putative peptidase lipoprotein YgeR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (29/25);  total (29/25)

ACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑CCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCC  >  NC_000913/2999610‑2999883                                                                                                                                             |                                                                                                                                       aCGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAt                                                                                                                                         <  1:260984/140‑1 (MQ=255)  cGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑c                                                                                                                                       >  1:677288/1‑140 (MQ=255)    tgtAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑ccc                                                                                                                                     <  2:92497/140‑1 (MQ=255)      tAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑ccccc                                                                                                                                   >  2:44138/1‑140 (MQ=255)              cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCa                                                                                                                            <  1:111276/140‑1 (MQ=255)              cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCa                                                                                                                            >  2:748220/1‑140 (MQ=255)                aCTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACg                                                                                                                          <  1:299749/140‑1 (MQ=255)                 cTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACgg                                                                                                                         >  1:675976/1‑140 (MQ=255)                 cTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACgg                                                                                                                         >  1:401887/1‑140 (MQ=255)                   tttCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGgtg                                                                                                                       <  1:405941/140‑1 (MQ=255)                    ttCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGgtgt                                                                                                                      >  1:49314/1‑140 (MQ=255)                    ttCGCCCCACCCAGTTTTAGTTTCAGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGgtgt                                                                                                                      >  2:113839/1‑140 (MQ=255)                            acccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGGGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAg                                                                                                              >  2:742844/1‑140 (MQ=255)                             cccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGc                                                                                                             <  2:25265/140‑1 (MQ=255)                             cccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGc                                                                                                             <  2:401887/140‑1 (MQ=255)                                    ttAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa                                                                                                      <  1:569997/140‑1 (MQ=255)                                    ttAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa                                                                                                      <  1:742844/140‑1 (MQ=255)                                    ttAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa                                                                                                      <  1:544292/140‑1 (MQ=255)                                     tAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATAc                                                                                                     >  1:529076/1‑140 (MQ=255)                                     tAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATAc                                                                                                     >  2:728009/1‑140 (MQ=255)                                        tttCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGtt                                                                                                  >  1:494386/1‑140 (MQ=255)                                          tCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTcc                                                                                                >  2:503272/1‑140 (MQ=255)                                              aCCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTa                                                                                            >  2:204071/1‑140 (MQ=255)                                               ccAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTAt                                                                                           <  1:728009/140‑1 (MQ=255)                                                 aaCTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCg                                                                                         <  1:390485/140‑1 (MQ=255)                                                    ttCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGgat                                                                                      <  1:503272/140‑1 (MQ=255)                                                            tgtAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCt                                                                              >  2:46026/1‑140 (MQ=255)                                                              tAAGGGGGGGAAATGCCGCTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTAc                                                                            >  2:526613/1‑140 (MQ=255)                                                                     gggAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACa                                                                     >  1:312973/1‑140 (MQ=255)                                                                      ggAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAg                                                                    >  2:251183/1‑140 (MQ=255)                                                                      ggAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAg                                                                    >  1:79558/1‑140 (MQ=255)                                                                       gAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGc                                                                   <  2:529076/140‑1 (MQ=255)                                                                       gAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGc                                                                   <  1:113839/140‑1 (MQ=255)                                                                               gTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCaaa                                                           <  1:748220/140‑1 (MQ=255)                                                                                  cAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGc                                                        >  1:617372/1‑140 (MQ=255)                                                                                     tCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGt                                                     <  1:41255/140‑1 (MQ=255)                                                                                         gcCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCaa                                                 <  2:4275/140‑1 (MQ=255)                                                                                           cAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAAcc                                               <  2:79558/140‑1 (MQ=255)                                                                                                 ttttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCGAAAGCAGTCCAACCGATaac                                         <  1:526613/140‑1 (MQ=255)                                                                                                   ttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATaacaa                                       <  1:251183/140‑1 (MQ=255)                                                                                                     aCGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTACTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAacaaca                                     <  2:812225/140‑1 (MQ=255)                                                                                                       gCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATaa                                          <  1:181893/133‑1 (MQ=255)                                                                                                       gCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATaa                                          >  2:181893/1‑133 (MQ=255)                                                                                                          ggTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACg                                <  1:483779/140‑1 (MQ=255)                                                                                                                cGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATAccc                          >  2:21946/1‑140 (MQ=255)                                                                                                                 gtggtgCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCa                         <  1:79832/140‑1 (MQ=255)                                                                                                                     tgCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAgaga                     >  2:135920/1‑140 (MQ=255)                                                                                                                      gcgcGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGAt                    >  2:674673/1‑140 (MQ=255)                                                                                                                       cgcgAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGAtt                   <  2:486887/140‑1 (MQ=255)                                                                                                                         cgAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCa                         >  1:122938/1‑132 (MQ=255)                                                                                                                         cgAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCa                         <  2:122938/132‑1 (MQ=255)                                                                                                                               aCGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTc           >  1:21298/1‑140 (MQ=255)                                                                                                                                      aGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTc    >  2:217958/1‑140 (MQ=255)                                                                                                                                      aGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTc    >  2:208557/1‑140 (MQ=255)                                                                                                                                        cGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATTGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTccc  >  1:278058/1‑140 (MQ=255)                                                                                                                                        cGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTccc  >  1:579120/1‑140 (MQ=255)                                                                                                                                        cGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTccc  >  1:23705/1‑140 (MQ=255)                                                                                                                                        cGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTccc  >  1:770675/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                       ACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑CCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCC  >  NC_000913/2999610‑2999883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑