| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,177,509 | (C)6→7 | coding (402/630 nt) | nudF ← | ADP‑sugar pyrophosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,177,503 | 1 | . | C | 89.7% | 117.1 / 7.6 | 39 | coding (408/630 nt) | nudF | ADP‑sugar pyrophosphatase |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base C (20/15); total (22/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑G‑‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAA > NC_000913/3177369‑3177627 | tctACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑G‑‑ccccc < 1:733289/140‑1 (MQ=255) aCTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCt < 1:661258/140‑1 (MQ=255) tGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTg > 1:132847/1‑140 (MQ=255) tGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTg > 1:488070/1‑140 (MQ=255) tGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTg < 1:274555/140‑1 (MQ=255) gTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑G‑C‑‑CCCCCCGGGCTTGCCAGGAAAC‑tt > 1:180190/1‑140 (MQ=255) cccGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTaa > 1:322943/1‑140 (MQ=255) cGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAaca < 2:322943/140‑1 (MQ=255) cGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAaca < 2:132847/140‑1 (MQ=255) cGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAaca < 1:575835/140‑1 (MQ=255) ggCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑G‑‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACAcc > 1:372552/1‑140 (MQ=255) aCCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGtt > 2:402159/1‑140 (MQ=255) ccACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑G‑C‑‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTg < 2:180190/140‑1 (MQ=255) cacaTGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGaaa > 2:741178/1‑125 (MQ=255) cacaTGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGaaa < 1:741178/125‑1 (MQ=255) caTGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGt < 1:662609/140‑1 (MQ=255) aTGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑G‑‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTcc < 2:372552/140‑1 (MQ=255) aaCGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑Gt > 1:89814/1‑140 (MQ=255) aaCGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑Gt < 1:530550/140‑1 (MQ=255) gcgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GCCCCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑Gttt < 1:690997/140‑1 (MQ=255) gcgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTg < 1:485836/140‑1 (MQ=255) gcgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTg < 2:180651/140‑1 (MQ=255) gcgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTg < 1:402159/140‑1 (MQ=255) cgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGa > 1:189119/1‑140 (MQ=255) cgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGa > 1:1683/1‑140 (MQ=255) gAATATCTTCGTTTTCATCAGCCAGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGAc < 2:189119/140‑1 (MQ=255) gAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGAc > 1:190725/1‑140 (MQ=255) tttttCATCAGCCAGACCGTGAATCCCGCTTGCGGTCGGGGCGCCCCCTCCCCCCACCATAATTGACGACCGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACCCCGGTTTGGTCC‑GTTTGACTATCAGTccc < 2:488070/139‑1 (MQ=255) tGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTcgc > 1:48460/1‑140 (MQ=255) aaTACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGa > 1:251698/1‑140 (MQ=255) ttGCGGTCGTGGCGTCCACTCCCCCCACCATAATTGACGACCGCTCACTGG‑TCC‑‑CCCCCCGGGCTTGCCAGAAAAC‑TTAACCCCGGTTTGGCCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAc < 2:587136/140‑1 (MQ=255) tGCGGTCGTGGCGTCCACTTCGCCCACCATAATTTACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACa > 2:155955/1‑140 (MQ=255) gCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACAt > 1:350272/1‑140 (MQ=255) gTGGCGCCCTCTTCCCCCACAATAATTGACGAACGCTCACTGG‑TCC‑‑CCCCCCGGGCTTGCCAGGAAAC‑TTACCCCCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCca < 2:48460/140‑1 (MQ=255) gcctccccccACAAAATTTGACGACCGCTCACTGG‑G‑C‑‑CCCCCCGGGGTTTCCCAGAAACTTTAACACCGG‑TTGGTCCTGTTTAACTACCGGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCa < 2:358991/133‑1 (MQ=255) cccTTCCCCCCCCATAATTGACGAACGCTCACTGGCC‑C‑‑CCCCCCGGGCTTCCCAGGAACC‑TCCACCCCGTTTTGGTCG‑GTTTGCCTATCAGCCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCa < 2:143434/138‑1 (MQ=255) ccccACCATAATTTACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCctct < 1:155955/139‑1 (MQ=255) ccccACCATAATTGACGAACGCTCACTGG‑TCC‑‑CCCCCCGGGCTTGCCAGAAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCctct < 2:89814/139‑1 (MQ=255) accaTAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCaa > 2:50247/1‑140 (MQ=255) ccaTAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAat > 1:246541/1‑140 (MQ=255) ccaTAATTGACAACCGCTCACTGG‑TCC‑‑CCCCCCGGGCTTGCAAAGAATC‑TTAACACCGTTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAat < 2:251698/140‑1 (MQ=255) aTAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAatct > 1:358991/1‑139 (MQ=255) gaccGCCCACTGG‑TCC‑‑CCCCCCGGGTTTGCCAGGAAAC‑TTACCCCCGTTTTGGTCC‑TTTTGACTACCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCaa < 2:189246/137‑1 (MQ=255) gAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGTCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCaa > 1:699722/1‑140 (MQ=255) gAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCaa > 1:779986/1‑140 (MQ=255) gAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCaa > 1:601048/1‑140 (MQ=255) gAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCaa > 1:189246/1‑140 (MQ=255) | TCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑G‑‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAA > NC_000913/3177369‑3177627 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |