Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	210,867	A→G	T397A (ACC→GCC)	ldcC →	lysine decarboxylase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	210,867	0	A	G	100.0%	111.5 / NA	33	T397A (ACC→GCC)	ldcC	lysine decarboxylase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/16);  total (17/16)

CGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGTTCAGTAGAACGAGCTCTGCATTTTCGCAAAGAGGTCCAG  >  NC_000913/210731‑210998                                                                                                                                         |                                                                                                                                    cgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATgcca                                                                                                                                  <  2:259122/140‑1 (MQ=255)     cGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATgccaccac                                                                                                                              <  2:271532/140‑1 (MQ=255)      gtgtTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATgccaccacc                                                                                                                             <  2:745720/140‑1 (MQ=255)         ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGGAGCCTTTATGATGCATGCCACCACCTCg                                                                                                                          <  1:386819/140‑1 (MQ=255)           gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGcc                                                                                                                        <  1:460560/140‑1 (MQ=255)             gggAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCa                                                                                                                      >  1:213437/1‑140 (MQ=255)             gggAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCa                                                                                                                      >  1:438980/1‑140 (MQ=255)                        tCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCAtt                                                                                                           <  2:82056/140‑1 (MQ=255)                                     aTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCAttgt                                                                                                         >  1:757477/1‑129 (MQ=255)                                     aTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCAttgt                                                                                                         <  2:757477/129‑1 (MQ=255)                                      tCGACCCACAAAATGCTGGCGGCGTTATCGCAGGTTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTga                                                                                             >  2:735023/1‑140 (MQ=255)                                         aCCCACAAAATGCTGGCAGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGAc                                                                                          >  1:280090/1‑140 (MQ=255)                                                      tGGCAGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGAtg                                                                             <  2:280090/140‑1 (MQ=255)                                                       ggcggcGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGAtgc                                                                            >  2:422531/1‑140 (MQ=255)                                                        gcggcgTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGAtgct                                                                           <  2:213437/140‑1 (MQ=255)                                                        gcggcgTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGAtgct                                                                           <  2:438980/140‑1 (MQ=255)                                                           gcgTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCg                                                                        >  2:505799/1‑140 (MQ=255)                                                                                     caTTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTa                                              >  2:318524/1‑140 (MQ=255)                                                                                          aaGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGt                                         >  1:755530/1‑140 (MQ=255)                                                                                          aaGGCGAGTATGACGAAGAGGCCTTTAACG‑AGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTaa                                             <  2:524875/135‑1 (MQ=255)                                                                                          aaGGCGAGTATGACGAAGAGGCCTTTAACG‑AGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTaa                                             >  1:524875/1‑135 (MQ=255)                                                                                           aGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGtt                                        <  1:505799/140‑1 (MQ=255)                                                                                                       cGAAGAGGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGTTCAGTAGAACGAg                            >  2:782220/1‑140 (MQ=255)                                                                                                          agagGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGTTCAGTAGAACGAGctc                         >  2:85475/1‑140 (MQ=255)                                                                                                          agagGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGTTCAGTAGAACGAGctc                         >  2:168141/1‑140 (MQ=255)                                                                                                          agagGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGTTCAGTAGAACGAGctc                         >  1:710126/1‑140 (MQ=255)                                                                                                            agGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGt                                         <  1:133886/122‑1 (MQ=255)                                                                                                            agGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGt                                         >  2:133886/1‑122 (MQ=255)                                                                                                            agGCCTTTAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGTTCAGTAGAACGAGCTCTg                       >  1:62108/1‑140 (MQ=255)                                                                                                                 tttAACGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGTTCAGTAGAACGAGCTCTGCAttt                  >  2:615471/1‑140 (MQ=255)                                                                                                                    aaCGAAGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGTTCAGTAGAACGAGCTCTGCATTTTCg               <  1:782220/140‑1 (MQ=255)                                                                                                                        aaGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGTTCAGTAGAACGAGCTCTGCATTTTCGCaaa           <  1:318524/140‑1 (MQ=255)                                                                                                                        aaGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGTTCAGTAGAACGAGCTCTGCATTTTCGCaaa           <  1:168141/140‑1 (MQ=255)                                                                                                                         aGCCTTTATGATGCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGTTCAGTAGAACGAGCTCTGCATTTTCGCAAag          <  1:615471/140‑1 (MQ=255)                                                                                                                                 tgatgCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGTTCAGTAGAACGAGCTCTGCATTTTCGCAAAGAGGTCCAg  <  2:239590/140‑1 (MQ=255)                                                                                                                                 tgatgCATGCCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGTTCAGTAGAACGAGCTCTGCATTTTCGCAAAGAGGTCCAg  <  1:85475/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGTTGAGACGGCGGCGGCGATGCTGCGTGGTAATCCGGGCAAACGGCTGATTAACCGTTCAGTAGAACGAGCTCTGCATTTTCGCAAAGAGGTCCAG  >  NC_000913/210731‑210998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑