Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,526,028	A→G	intergenic (‑48/‑219)	ansP ← / → yncG	L‑asparagine transporter/putative glutathione S‑transferase YncG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,526,028	0	A	G	100.0%	152.7 / NA	44	intergenic (‑48/‑219)	ansP/yncG	L‑asparagine transporter/putative glutathione S‑transferase YncG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/22);  total (22/22)

TCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGATTAA  >  NC_000913/1525893‑1526159                                                                                                                                        |                                                                                                                                    tCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAAt                                                                                                                                 <  1:638800/140‑1 (MQ=255) tCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGCTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAAt                                                                                                                                 <  2:554864/140‑1 (MQ=255)  cGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATg                                                                                                                                <  2:280291/140‑1 (MQ=255)       tgtgATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTAt                                                                                                                           <  2:432908/140‑1 (MQ=255)            tACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATc                                                                                                                      <  2:784848/140‑1 (MQ=255)               cccTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTg                                                                                                                   >  2:511990/1‑140 (MQ=255)                 ctctTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGc                                                                                                                 >  1:57411/1‑140 (MQ=255)                   ctTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCga                                                                                                               >  2:332194/1‑140 (MQ=255)                      cGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCgatga                                                                                                            >  1:256621/1‑140 (MQ=255)                           gCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGtt                                                                                                       >  2:718597/1‑140 (MQ=255)                            cATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTTGGTGTCGTGTTTACTAATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTa                                                                                                      >  1:466684/1‑140 (MQ=255)                             aTTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATGAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTAt                                                                                                     <  2:359167/140‑1 (MQ=255)                             aTTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTAt                                                                                                     <  1:11113/140‑1 (MQ=255)                                  gCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCa                                                                                                <  1:511990/140‑1 (MQ=255)                                                              tGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCgg                                                                    >  2:632675/1‑140 (MQ=255)                                                                           tcgTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTAc                                                       <  1:632675/140‑1 (MQ=255)                                                                            cgTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTAcc                                                      >  1:525256/1‑140 (MQ=255)                                                                               gtTTACTAATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGt                                                   <  2:466684/140‑1 (MQ=255)                                                                                   aCTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCgcagc                                                              >  1:198733/1‑125 (MQ=255)                                                                                   aCTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCgcagc                                                              <  2:198733/125‑1 (MQ=255)                                                                                     tCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGa                                             >  2:213439/1‑140 (MQ=255)                                                                                         tGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATaaa                                         >  2:456711/1‑140 (MQ=255)                                                                                             ctcCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATaaa                                         >  1:94998/1‑136 (MQ=255)                                                                                             ctcCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATaaa                                         <  2:94998/136‑1 (MQ=255)                                                                                             ctcCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGAt                                     >  1:196703/1‑140 (MQ=255)                                                                                             ctcCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGAt                                     <  2:160042/140‑1 (MQ=255)                                                                                                 cTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCaa                                 >  2:585216/1‑140 (MQ=255)                                                                                                   gATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCaaaa                               >  1:386282/1‑140 (MQ=255)                                                                                                      tGCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACAt                            <  1:711789/140‑1 (MQ=255)                                                                                                       gCTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACATc                           <  2:256621/140‑1 (MQ=255)                                                                                                        cTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGa                                      <  2:335490/128‑1 (MQ=255)                                                                                                        cTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGa                                      >  1:335490/1‑128 (MQ=255)                                                                                                        cTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACATcc                          <  1:718597/140‑1 (MQ=255)                                                                                                        cTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACATcc                          <  2:386282/140‑1 (MQ=255)                                                                                                        cTTTAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACATcc                          <  1:21096/140‑1 (MQ=255)                                                                                                          ttAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACATcccc                        >  1:398403/1‑140 (MQ=255)                                                                                                           tAATGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACATCCCCt                       <  2:143572/140‑1 (MQ=255)                                                                                                              tGAAAAAGTCATATAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACATCCCCTTAc                    >  1:615001/1‑140 (MQ=255)                                                                                                                        atatAAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACATCCCCTTACAATCCTGAAg          >  2:685947/1‑140 (MQ=255)                                                                                                                           taAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACTCGTTATCGATAAACGATGCAAAACATCCCCTTACAATCCTGAAgggg       <  2:398403/140‑1 (MQ=255)                                                                                                                           taAGTTGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACATCCCCTTACAATCCTGAAgggg       <  2:525256/140‑1 (MQ=255)                                                                                                                               ttGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGATTa   >  2:436854/1‑140 (MQ=255)                                                                                                                               ttGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGATTa   >  2:484540/1‑140 (MQ=255)                                                                                                                               ttGCCATGAGCAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACAT‑CCCTTACAATCCTGAAGGGGATTaa  >  2:676504/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    TCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGATTAA  >  NC_000913/1525893‑1526159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑