Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,546,963	A→G	S69S (AGT→AGC)	yddG ←	aromatic amino acid exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,546,963	0	A	G	100.0%	190.1 / NA	55	S69S (AGT→AGC)	yddG	aromatic amino acid exporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/30);  total (25/30)

CATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAA  >  NC_000913/1546827‑1547099                                                                                                                                         |                                                                                                                                         cATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTc                                                                                                                                       <  1:55277/140‑1 (MQ=255)   ttAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTccc                                                                                                                                     <  1:381952/140‑1 (MQ=255)    tAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCg                                                                                                                                    >  1:642883/1‑140 (MQ=255)    tAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCg                                                                                                                                    >  1:469301/1‑140 (MQ=255)        cAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGa                                                                                                                                <  2:642883/140‑1 (MQ=255)           aaTGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGTATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTa                                                                                                                             >  2:725110/1‑140 (MQ=255)             tGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTaaa                                                                                                                           >  1:583142/1‑140 (MQ=255)                cAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCg                                                                                                                                    >  2:297978/1‑128 (MQ=255)                cAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCg                                                                                                                                    <  1:297978/128‑1 (MQ=255)                cAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAg                                                                                                                        <  1:790673/140‑1 (MQ=255)                cAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAg                                                                                                                        <  1:269424/140‑1 (MQ=255)                cAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAg                                                                                                                        <  1:718981/140‑1 (MQ=255)                     agaATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCttt                                                                                                                   >  1:729010/1‑140 (MQ=255)                      gaATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTc                                                                                                                  <  2:469301/140‑1 (MQ=255)                                              aGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAAt                                                                                          >  1:433800/1‑140 (MQ=255)                                               gTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATc                                                                                         <  1:322749/140‑1 (MQ=255)                                                ttCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATcc                                                                                        <  2:729010/140‑1 (MQ=255)                                                  cACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGTATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCaa                                                                                      <  1:725110/140‑1 (MQ=255)                                                   aCCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAAc                                                                                     >  2:638881/1‑140 (MQ=255)                                                     cATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATTCGCGGAAATCCAACCg                                                                                   <  2:650887/140‑1 (MQ=255)                                                      aTACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATcc                                                                                        >  2:302855/1‑134 (MQ=255)                                                      aTACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATcc                                                                                        <  1:302855/134‑1 (MQ=255)                                                       tACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTg                                                                                 <  2:433800/140‑1 (MQ=255)                                                        aCCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGa                                                                                >  2:697314/1‑140 (MQ=255)                                                        aCCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGa                                                                                <  1:697314/140‑1 (MQ=255)                                                        aCCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGa                                                                                >  1:272359/1‑140 (MQ=255)                                                        aCCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGa                                                                                <  1:638881/140‑1 (MQ=255)                                                            aCTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGAt                                                                            <  1:204104/140‑1 (MQ=255)                                                             cTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCa                                                                                       <  1:596316/128‑1 (MQ=255)                                                             cTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCa                                                                                       >  2:596316/1‑128 (MQ=255)                                                               tCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTaa                                                                         >  1:766745/1‑140 (MQ=255)                                                               tCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTaa                                                                         >  1:358562/1‑140 (MQ=255)                                                                   tCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAAcagc                                                                     >  1:470751/1‑140 (MQ=255)                                                                    cGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAAcagca                                                                    >  1:289299/1‑140 (MQ=255)                                                                          atgatgGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGc                                                              <  1:347136/140‑1 (MQ=255)                                                                          atgatgGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGc                                                              >  2:477526/1‑140 (MQ=255)                                                                            gatgGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCtt                                                            <  2:272359/140‑1 (MQ=255)                                                                            gatgGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCtt                                                            <  1:477526/140‑1 (MQ=255)                                                                               gggTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAAt                                                         >  2:447895/1‑140 (MQ=255)                                                                                  tCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGaa                                                      <  1:216549/140‑1 (MQ=255)                                                                                  tCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGaa                                                      <  2:470751/140‑1 (MQ=255)                                                                                     ccgcATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGccc                                                                >  2:335401/1‑127 (MQ=255)                                                                                     ccgcATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGccc                                                                <  1:335401/127‑1 (MQ=255)                                                                                     ccgcATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAAt                                                         <  2:192249/134‑1 (MQ=255)                                                                                     ccgcATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAAt                                                         >  1:192249/1‑134 (MQ=255)                                                                                       gcATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAatagat                                                 <  1:746185/140‑1 (MQ=255)                                                                                                       gcgcCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGccgccg                                 >  2:256487/1‑140 (MQ=255)                                                                                                       gcgcCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGccgccg                                 >  1:595097/1‑140 (MQ=255)                                                                                                       gcgcCAGACAGATTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGccgccg                                 >  2:13614/1‑140 (MQ=255)                                                                                                                  aTTTCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAg                      >  1:1291/1‑140 (MQ=255)                                                                                                                     tCATAGCTGACGAATAACAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGccc                   <  1:131986/140‑1 (MQ=255)                                                                                                                                    aaCAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGc                     <  2:663313/123‑1 (MQ=255)                                                                                                                                    aaCAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGc                     >  1:663313/1‑123 (MQ=255)                                                                                                                                     aCAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGa   <  2:595097/140‑1 (MQ=255)                                                                                                                                      cAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaa  <  1:24223/140‑1 (MQ=255)                                                                                                                                      cAGGCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaa  <  1:386854/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAA  >  NC_000913/1546827‑1547099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑