Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,599,454	G→A	G252G (GGC→GGT)	lsrK ←	autoinducer‑2 kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,599,454	0	G	A	100.0%	139.8 / NA	45	G252G (GGC→GGT)	lsrK	autoinducer‑2 kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/24);  total (21/24)

GGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCTAAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTAGGCCAGCCATA  >  NC_000913/1599317‑1599583                                                                                                                                          |                                                                                                                                  ggATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCTAAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCAcc                                                                                                                                 >  1:282912/1‑140 (MQ=255) ggATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCTAAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCAcc                                                                                                                                 >  2:202800/1‑140 (MQ=255) ggATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCTAAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCAcc                                                                                                                                 >  1:533658/1‑140 (MQ=255) ggATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCTAAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCAcc                                                                                                                                 >  2:648218/1‑140 (MQ=255)  gATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCTAAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCAcct                                                                                                                                <  1:820631/140‑1 (MQ=255)       aCGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCTAAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCtcctcc                                                                                                                           >  1:639074/1‑140 (MQ=255)          cgcACGTTCATTTCTGGGTCTGTCACCGGCGCGGCTAAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAAc                                                                                                                        <  1:219008/140‑1 (MQ=255)          cgcACGTTCATTTCTGGGTCTGTCACCGGCGCGGCTAAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAAc                                                                                                                        <  1:202800/140‑1 (MQ=255)            cACGTTCATTTCTGGGTCTGTCACCGGCGCGGCTAAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGa                                                                                                                      >  2:125535/1‑140 (MQ=255)                     ttCTGGGTCTGTCACCGGCGCGGCTAAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAg                                                                                                             <  2:670378/140‑1 (MQ=255)                         gggTCTGTCACCGGCGCGGCTAAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGcc                                                                                                         >  1:749660/1‑140 (MQ=255)                               gTCACCGGCGCGGCTAAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCtt                                                                                                   >  2:724291/1‑140 (MQ=255)                                tCACCGGCGCGGCTAAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTc                                                                                                  <  2:639074/140‑1 (MQ=255)                                      gcgcGGCTAAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCg                                                                                            >  1:214064/1‑140 (MQ=255)                                                aTTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTccg                                                                                  <  1:244373/140‑1 (MQ=255)                                                 tttACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTccgc                                                                                 <  2:473575/140‑1 (MQ=255)                                                      aaCTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCtt                                                                            >  2:317769/1‑140 (MQ=255)                                                           gctgcCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCCCGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGaa                                                                       <  1:724291/140‑1 (MQ=255)                                                           gctgcCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGaa                                                                       >  1:16602/1‑140 (MQ=255)                                                           gctgcCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGaa                                                                       >  1:672895/1‑140 (MQ=255)                                                                 aGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTAcc                                                                 <  1:317769/140‑1 (MQ=255)                                                                   aaTGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCAc                                                               <  2:214064/140‑1 (MQ=255)                                                                             cAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGcc                                                            <  1:391877/133‑1 (MQ=255)                                                                             cAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGcc                                                            >  2:391877/1‑133 (MQ=255)                                                                                gAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAAtgtg                                                  <  1:153279/140‑1 (MQ=255)                                                                                           tgtgCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCttt                                       >  2:90382/1‑140 (MQ=255)                                                                                           tgtgCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCttt                                       >  1:236769/1‑140 (MQ=255)                                                                                             tgCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGa                                     <  1:705018/140‑1 (MQ=255)                                                                                             tgCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGa                                     <  2:533658/140‑1 (MQ=255)                                                                                             tgCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGa                                     <  2:635142/140‑1 (MQ=255)                                                                                                    cGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGgaga                              <  1:90382/140‑1 (MQ=255)                                                                                                       acaACGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGagaaag                           >  1:258941/1‑140 (MQ=255)                                                                                                          aCGCCTAACCCAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAat                        >  1:217284/1‑140 (MQ=255)                                                                                                                   ccAAGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACg               <  1:731875/140‑1 (MQ=255)                                                                                                                     aaGGCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTa             >  1:210653/1‑140 (MQ=255)                                                                                                                       ggCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTAg            <  1:646608/139‑1 (MQ=255)                                                                                                                       ggCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTAg            >  2:646608/1‑139 (MQ=255)                                                                                                                        gCAACCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTAGgc          <  1:540017/140‑1 (MQ=255)                                                                                                                           aCCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCTGCACGTAGgccag       <  1:125535/140‑1 (MQ=255)                                                                                                                           aCCAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTAGgccag       <  2:749660/140‑1 (MQ=255)                                                                                                                            ccAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACg               <  2:545375/131‑1 (MQ=255)                                                                                                                            ccAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACg               >  1:545375/1‑131 (MQ=255)                                                                                                                            ccAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTAGgccagc      >  2:598161/1‑140 (MQ=255)                                                                                                                             cAAGCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTAGgccagcc     <  2:16602/140‑1 (MQ=255)                                                                                                                                gCTGCACGTCACCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTAGGCCAGCCata  <  2:232383/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  GGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCTAAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTAGGCCAGCCATA  >  NC_000913/1599317‑1599583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑