Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	256,653	A→G	T43A (ACC→GCC)	frsA →	fermentation‑respiration switch protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	256,653	0	A	G	100.0%	106.7 / NA	31	T43A (ACC→GCC)	frsA	fermentation‑respiration switch protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/15);  total (16/15)

GCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATG  >  NC_000913/256518‑256776                                                                                                                                        |                                                                                                                            gCTCCATCCATGACACGGAAAACCCTGAGCGAACCCCTCTCCAACCCCCGTTTAAAACATCCGAAACCCCCGACGTTGGCCCGCCGTTTTAACCCCGGCGCAAAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCAt                                                                                                                         <  2:363305/140‑1 (MQ=255)   tGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATcc                                                                                                                       >  2:776252/1‑140 (MQ=255)     cATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCt                                                                                                                     >  1:372922/1‑140 (MQ=255)           agACACAGGCAACCCGGAGCAAACCCCTTTTAAACCCCCGTTTAAAACATCCTTAACCCCCGACGCTAGCCCGCCTTTTTAATCACGGCCCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTgg                                                                                                               <  2:723639/139‑1 (MQ=255)                aaGGAAAACCGGAGCAAACCCCTTTTAAACCCCCGTTTAAAACATCCTAAACCCTCGACGCTAGCCCGCCGTTTAAATCACGGCGCAAAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCg                                                                                                          <  2:316160/139‑1 (MQ=255)                   gCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCAt                                                                                                       >  1:27317/1‑140 (MQ=255)                   gCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCAt                                                                                                       >  1:776281/1‑140 (MQ=255)                            aGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCg                                                                                              >  1:609520/1‑140 (MQ=255)                             gCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGt                                                                                             >  1:273735/1‑140 (MQ=255)                                               ccccttttAACCACCCTAAACCCCCGCCGCTAGCCCGCCGTTTAAACCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGgcgc                                                                           <  2:103901/135‑1 (MQ=255)                                                  gCTTTAAACATCCTGAAACCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGc                                                                        >  1:100219/1‑140 (MQ=255)                                                         acacCCTGAACCCTCGACGCTAGCCCGCCGTTTTAACCCCGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGAcc                                                                 <  1:44502/140‑1 (MQ=255)                                                             ccTGAAACCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCAcg                                                             >  1:43080/1‑140 (MQ=255)                                                                  aaCCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGaaa                                                        >  2:144755/1‑140 (MQ=255)                                                                     cTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATcc                                                     >  2:535310/1‑140 (MQ=255)                                                                        gACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCg                                                  >  1:344994/1‑140 (MQ=255)                                                                             taccccgcctTTTTAACCCCGGCGCACACCCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTc                                             <  2:609520/136‑1 (MQ=255)                                                                             tAGCCCGCCGTTTTAACCCCGGCCCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTc                                             <  2:216680/140‑1 (MQ=255)                                                                               gcccgccgCTTTAATCGCGGCCCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCa                                           <  1:757778/138‑1 (MQ=255)                                                                                  cgccgcTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGc                                        <  2:344994/140‑1 (MQ=255)                                                                                                 ggCGCAAAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAg                         <  1:769221/140‑1 (MQ=255)                                                                                                                gtgCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTAc          >  1:757693/1‑140 (MQ=255)                                                                                                                 tgCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTAcc         <  2:43080/140‑1 (MQ=255)                                                                                                                  gCAGTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACcg        >  1:713446/1‑140 (MQ=255)                                                                                                                     gTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACcgacg     <  1:43187/140‑1 (MQ=255)                                                                                                                     gTCGGCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACcgacg     <  2:757693/140‑1 (MQ=255)                                                                                                                        ggCCCTTGATGTTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTTATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACcgacg     <  2:509374/137‑1 (MQ=255)                                                                                                                        ggCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTTATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACcgacg     >  1:509374/1‑137 (MQ=255)                                                                                                                        ggCCCTTGATGGTAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACgatg  >  1:212819/1‑140 (MQ=255)                                                                                                                                    tAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACcgacg     <  1:727738/125‑1 (MQ=255)                                                                                                                                    tAAAGCCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACcgacg     >  2:727738/1‑125 (MQ=255)                                                                                                                                        |                                                                                                                            GCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATG  >  NC_000913/256518‑256776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑