| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,635,861 | T→C | I169M (ATA→ATG) | nohA ← | Qin prophage; putative prophage DNA‑packaging protein NohA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,635,861 | 0 | T | C | 95.3% | 127.6 / ‑2.4 | 43 | I169M (ATA→ATG) | nohA | Qin prophage; putative prophage DNA‑packaging protein NohA |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (24/17); total (26/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
CGGCTGTCATCCCTTTCAGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTTATACACCTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATC > NC_000913/1635724‑1635975 | cGGCTGTCATCCCTTTCAGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTTATACACCTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCAt > 1:686690/1‑140 (MQ=255) tGTCATCCCTTTCAGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTTATACACCTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGt > 1:433403/1‑140 (MQ=255) cATCCCTTTCAGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTTATACACCTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCa < 1:231248/140‑1 (MQ=255) cATCCCTTTCAGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTTATACACCTCGTATAACGCAAGACCTGCTGCCCCCAGCGTATTGTCTGTCAGCGCGGTTATATTCACTGAGCAACCCCGGCATCAGTTCa > 2:754335/1‑140 (MQ=255) ccTTTCAGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTTATACACCTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCa > 1:738512/1‑140 (MQ=255) ttCAGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTTATACACCTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAgcg < 1:809291/140‑1 (MQ=255) aGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTTATACACCTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCgg < 1:128959/140‑1 (MQ=255) tCCATACGCTGTTGGATCTCCGTTGTTATACACCTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCATCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATg < 2:738512/140‑1 (MQ=255) tACGCTGTTGGATATCCGTTGTTATACACCTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCtt < 1:754335/140‑1 (MQ=255) aCGCTGTTGGATATACGTTGTTATACACCTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCttt > 2:399822/1‑140 (MQ=255) aCGCTGTTGGATATACGTTGTTATACACCTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCttt < 1:399822/140‑1 (MQ=255) gttATACACCTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAgg > 1:706993/1‑140 (MQ=255) gttATACACCTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAgg < 1:753651/140‑1 (MQ=255) cacCTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCa > 2:616255/1‑140 (MQ=255) cTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTGAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACa > 2:381768/1‑140 (MQ=255) cTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACa > 2:337255/1‑140 (MQ=255) cTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACa > 1:789213/1‑140 (MQ=255) cTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACa > 2:611116/1‑140 (MQ=255) cGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCCGCGCGCTTATATTCACTCAGCAACCCCGGTATCAGAGCATCCAGCGCGGCGGCTTTGTTCATAGCTTTGCTGATATCCCGTTTCAGGAGATCAACacg > 2:67680/1‑138 (MQ=21) cGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATg > 1:447108/1‑140 (MQ=255) cGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATg > 2:319943/1‑140 (MQ=255) cGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATg > 2:123893/1‑140 (MQ=255) cGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATg > 1:663366/1‑140 (MQ=255) aaCTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGt > 1:621205/1‑140 (MQ=255) cTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGttt > 1:827603/1‑140 (MQ=255) tCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCgg < 1:603558/136‑1 (MQ=255) tCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCgg > 2:603558/1‑136 (MQ=255) cAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTc < 2:789213/140‑1 (MQ=255) cAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTc < 1:616255/140‑1 (MQ=255) gctgcCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGt > 1:18563/1‑124 (MQ=255) gctgcCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGt < 2:18563/124‑1 (MQ=255) gctgcCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCg > 1:828858/1‑140 (MQ=255) gctgcCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCg > 1:555897/1‑140 (MQ=255) ctgcCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCgg > 2:2249/1‑140 (MQ=255) gcCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGaa > 2:682157/1‑140 (MQ=255) cccTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAAc < 2:706993/140‑1 (MQ=255) gCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAAcgccg < 2:827603/140‑1 (MQ=255) ttGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCa < 2:447108/140‑1 (MQ=255) gtcAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACa > 2:610515/1‑140 (MQ=255) gtcAGCGCGGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACa > 1:445326/1‑140 (MQ=255) cgcgGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCAcc < 2:344790/131‑1 (MQ=21) cgcgGTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCAcc > 1:344790/1‑131 (MQ=21) ggTTATATTCACTCATCAACCCCAGCATCAGTGCATCCAGCGCGGCTGCTTTGTTCATGGCTTAGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTGCCGGAAAACGCCGCTGCGCCGACAGGGGGATc < 1:805878/140‑1 (MQ=11) ggTTATATTCACTCAGCAACCCCGGCATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATc < 1:611116/140‑1 (MQ=17) | CGGCTGTCATCCCTTTCAGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTTATACACCTCGTATAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATC > NC_000913/1635724‑1635975 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |