Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,666,097	A→G	A261A (GCA→GCG)	clcB →	putative chloride:H(+) antiporter ClcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,666,097	0	A	G	100.0%	223.9 / NA	65	A261A (GCA→GCG)	clcB	putative chloride:H(+) antiporter ClcB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/36);  total (29/36)

TTCAGGCTCGTGACTATGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTG  >  NC_000913/1665961‑1666232                                                                                                                                         |                                                                                                                                        ttCAGGCTCGTGACTATGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCACCGCCCTGGCAACTGGCGCTa                                                                                                                                      >  1:606940/1‑140 (MQ=255)   cAGGCTCGTGACTATGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCACCGCCCTGGCAACTGGCGCTAgg                                                                                                                                    <  2:606940/140‑1 (MQ=255)               tATGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGt                                                                                                                        >  2:37187/1‑140 (MQ=255)                 tGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTgg                                                                                                                      >  1:59854/1‑140 (MQ=255)                            tCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTcc                                                                                                           <  1:438771/140‑1 (MQ=255)                                tACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGt                                                                                                       >  1:316080/1‑140 (MQ=255)                                tACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTT‑ACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGTGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGtt                                                                                                      <  1:448193/140‑1 (MQ=255)                                  cAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTc                                                                                                     >  2:467612/1‑140 (MQ=255)                                     gtgtGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTcaca                                                                                                  >  2:639079/1‑140 (MQ=255)                                     gtgtGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTcaca                                                                                                  >  2:620865/1‑140 (MQ=255)                                     gtgtGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTcaca                                                                                                  >  1:299695/1‑140 (MQ=255)                                      tgtgCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCacac                                                                                                 <  1:662515/140‑1 (MQ=255)                                         gCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTg                                                                                              <  2:461659/140‑1 (MQ=255)                                         gCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTg                                                                                              >  2:435835/1‑140 (MQ=255)                                         gCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTg                                                                                              <  2:59854/140‑1 (MQ=255)                                              cAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAgtg                                                                                         <  1:435835/140‑1 (MQ=255)                                               aGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAgtgt                                                                                        >  2:643612/1‑140 (MQ=255)                                                  tCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTggg                                                                                     >  1:45547/1‑140 (MQ=255)                                                    tgtgCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGc                                                                                   >  1:457758/1‑140 (MQ=255)                                                     gtgCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCa                                                                                  <  1:377590/140‑1 (MQ=255)                                                     gtgCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCa                                                                                  <  1:639079/140‑1 (MQ=255)                                                      tgCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCaa                                                                                 >  2:795672/1‑140 (MQ=255)                                                       gCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAAc                                                                                <  1:643612/140‑1 (MQ=255)                                                       gCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAAc                                                                                <  2:181101/140‑1 (MQ=255)                                                        cGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACg                                                                               >  2:288590/1‑140 (MQ=255)                                                           aCCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAAc                                                                                >  1:594339/1‑136 (MQ=255)                                                           aCCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAAc                                                                                <  2:594339/136‑1 (MQ=255)                                                            ccACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCta                                                                           <  1:620865/140‑1 (MQ=255)                                                                tgttgtTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGc                                                                       >  2:546662/1‑140 (MQ=255)                                                                tgttgtTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGc                                                                       >  1:748320/1‑140 (MQ=255)                                                                tgttgtCAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGc                                                                       >  1:143886/1‑140 (MQ=255)                                                                 gttgttAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCa                                                                      <  1:688124/140‑1 (MQ=255)                                                                 gttgttAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCa                                                                      <  2:748320/140‑1 (MQ=255)                                                                 gttgttAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCa                                                                      <  2:45547/140‑1 (MQ=255)                                                                   tgttAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCAcc                                                                    <  1:288590/140‑1 (MQ=255)                                                                            tAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCta                                                                           >  1:268998/1‑124 (MQ=255)                                                                            tAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCta                                                                           <  2:268998/124‑1 (MQ=255)                                                                             aaTGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCt                                                          >  2:640006/1‑140 (MQ=255)                                                                                  aCGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTa                                                     <  1:37187/140‑1 (MQ=255)                                                                                   cGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTaa                                                    >  2:254/1‑140 (MQ=255)                                                                                    gCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAAc                                                   <  1:795672/140‑1 (MQ=255)                                                                                    gCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAAc                                                   <  1:254/140‑1 (MQ=255)                                                                                    gCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAAc                                                   <  2:299695/140‑1 (MQ=255)                                                                                        gTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGcc                                               >  1:629358/1‑140 (MQ=255)                                                                                        gTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGcc                                               >  1:547510/1‑140 (MQ=255)                                                                                        gTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGcc                                               >  1:22518/1‑140 (MQ=255)                                                                                         tCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGccc                                              <  1:793778/140‑1 (MQ=255)                                                                                            tCGTGGATTTGTGAGTCTCAACCTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGTTCGTGGTTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCccac                                           <  2:509313/140‑1 (MQ=255)                                                                                            tCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCccac                                           <  1:152311/140‑1 (MQ=255)                                                                                             cGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCccacc                                          <  2:629358/140‑1 (MQ=255)                                                                                              gTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCccacca                                         <  2:457758/140‑1 (MQ=255)                                                                                              gTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCccacca                                         <  2:22518/140‑1 (MQ=255)                                                                                              gTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCccacca                                         <  1:350808/140‑1 (MQ=255)                                                                                              gTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCccacca                                         <  2:547510/140‑1 (MQ=255)                                                                                              gTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCccacca                                         <  1:640006/140‑1 (MQ=255)                                                                                                             tcAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCccacca                                         >  2:327896/1‑125 (MQ=255)                                                                                                             tcAAACTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCccacca                                         <  1:327896/125‑1 (MQ=255)                                                                                                                 aCTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCg                      <  2:143886/140‑1 (MQ=255)                                                                                                                 aCTTGCGCCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCg                      <  1:546662/140‑1 (MQ=255)                                                                                                                     gcgcCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGAt                  >  1:686509/1‑140 (MQ=255)                                                                                                                     gcgcCGCCCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGAt                  >  2:152255/1‑140 (MQ=255)                                                                                                                        ccgccCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCtt               <  1:467612/140‑1 (MQ=255)                                                                                                                        ccgccCTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCtt               <  2:686509/140‑1 (MQ=255)                                                                                                                            ccTGGCAACTGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCtt           >  1:174298/1‑140 (MQ=255)                                                                                                                                     tGGCGCTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACtg  >  2:347107/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TTCAGGCTCGTGACTATGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTG  >  NC_000913/1665961‑1666232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑