Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,690,148	T→C	G99G (GGT→GGC)	ydgA →	conserved protein YdgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,690,148	0	T	C	100.0%	83.0 / NA	25	G99G (GGT→GGC)	ydgA	conserved protein YdgA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/16);  total (9/16)

AGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCACTGTTTGATATGGCAAAAGGTGAAACGCCTTT  >  NC_000913/1690017‑1690273                                                                                                                                    |                                                                                                                              aGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTccc                                                                                                                       >  1:172395/1‑140 (MQ=255) aGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTccc                                                                                                                       >  1:297914/1‑140 (MQ=255)   ttATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGc                                                                                                                     <  2:145420/140‑1 (MQ=255)       cAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGc                                                                                                                 <  1:98726/140‑1 (MQ=255)        aaaaCTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGcc                                                                                                                <  1:740298/140‑1 (MQ=255)        aaaaCTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGcc                                                                                                                <  1:763093/140‑1 (MQ=255)                  tcGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGGGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTaaaa                                                                                                      >  2:412843/1‑140 (MQ=255)                        cgTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTCGCCCAGCTTAAAAAACTGa                                                                                                <  1:297171/140‑1 (MQ=255)                         gTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGaa                                                                                               >  1:605955/1‑140 (MQ=255)                                            aaCTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGGATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCa                                                                            <  1:783442/140‑1 (MQ=255)                                                       aaaCCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAAc                                                                 <  2:20055/140‑1 (MQ=255)                                                         aCCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCa                                                               >  2:290496/1‑140 (MQ=255)                                                           ccATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACg                                                             <  2:559649/140‑1 (MQ=255)                                                           ccATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACg                                                             <  1:489350/140‑1 (MQ=255)                                                                   gggAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCAGTCGATGGCATCAATTCAAACCACGCTGGTtaa                                                     >  1:97005/1‑140 (MQ=255)                                                                      aaagaaaATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTtaataa                                                  <  1:220202/140‑1 (MQ=255)                                                                               ccGTGGATTAAAAGCGGTCAGGGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAg                                         <  1:412843/140‑1 (MQ=255)                                                                                    gATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAAc                                    <  1:667708/140‑1 (MQ=255)                                                                                      ttAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCa                                  <  2:293595/140‑1 (MQ=255)                                                                                               gTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCACTGTTTGat                         >  2:790793/1‑140 (MQ=255)                                                                                                 cAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCa                                  >  2:322730/1‑129 (MQ=255)                                                                                                 cAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCa                                  <  1:322730/129‑1 (MQ=255)                                                                                                                     aTCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCACTGTTTGATATGGCAAAAGGTGAAACGCCtt   >  2:450959/1‑140 (MQ=255)                                                                                                                      tCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCACTGTTTGATATGGCAAAAGGTGAAACGCCttt  <  1:290496/140‑1 (MQ=255)                                                                                                                      tCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCACTGTTTGATATGGCAAAAGGTGAAACGCCttt  <  2:607450/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                              AGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCACTGTTTGATATGGCAAAAGGTGAAACGCCTTT  >  NC_000913/1690017‑1690273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑