| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,721,339 | T→C | W26R (TGG→CGG) | ydhJ → | putative membrane fusion protein YdhJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,721,339 | 0 | T | C | 97.5% | 133.4 / ‑5.8 | 40 | W26R (TGG→CGG) | ydhJ | putative membrane fusion protein YdhJ |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (21/18); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGG > NC_000913/1721206‑1721471 | tATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAatt > 2:768619/1‑140 (MQ=255) tttGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATg > 1:557948/1‑140 (MQ=255) cGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAAt < 2:361178/140‑1 (MQ=255) cGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAAt < 1:708774/140‑1 (MQ=255) gCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGt > 2:142876/1‑140 (MQ=255) ttGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACAcgcg < 1:768619/140‑1 (MQ=255) gCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGaaa > 2:133956/1‑140 (MQ=255) aTTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGa < 2:694678/124‑1 (MQ=255) aTTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGa > 1:694678/1‑124 (MQ=255) ttGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAg > 1:235717/1‑140 (MQ=255) ttGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAg > 1:254184/1‑140 (MQ=255) gTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACa > 1:670818/1‑140 (MQ=255) ggTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAgg < 1:133956/140‑1 (MQ=255) aTGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTAt < 1:43962/140‑1 (MQ=255) gTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAgg > 1:821363/1‑131 (MQ=255) gTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAgg < 2:821363/131‑1 (MQ=255) aaTTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCAccc > 2:69965/1‑140 (MQ=255) aaTTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCAccc > 2:384453/1‑140 (MQ=255) tAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCcac < 1:667283/140‑1 (MQ=255) tATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACg > 1:808313/1‑140 (MQ=255) tttCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTa < 2:580160/140‑1 (MQ=255) aacaaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTAttgttg > 1:402949/1‑140 (MQ=255) aacaaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTAttgttg > 1:172242/1‑140 (MQ=255) caaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCggg < 1:29959/127‑1 (MQ=255) caaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCggg > 2:29959/1‑127 (MQ=255) caaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAg < 2:808313/140‑1 (MQ=255) caaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAg < 2:670818/140‑1 (MQ=255) caaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAg < 2:557948/140‑1 (MQ=255) tCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCggg < 1:153431/124‑1 (MQ=255) tCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCggg > 2:153431/1‑124 (MQ=255) tCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTg > 2:813104/1‑140 (MQ=255) tGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAata > 2:548460/1‑140 (MQ=255) gTAGTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGa > 2:716920/1‑140 (MQ=255) gTTGCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACaa > 1:85722/1‑140 (MQ=255) gCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCa > 2:828754/1‑140 (MQ=255) gCGGTTCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGGGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCa > 2:251560/1‑140 (MQ=255) tCTTGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTgg > 1:258412/1‑140 (MQ=255) ttGCCGGATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGtt < 2:235717/140‑1 (MQ=255) ggATGGTGGCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAAcgc < 1:548460/140‑1 (MQ=255) ggtggCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCgggg < 1:813104/140‑1 (MQ=255) gtggCTGCGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCggggg < 2:258412/140‑1 (MQ=255) | TATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGG > NC_000913/1721206‑1721471 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |