Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,743,648 G→A L64L (CTG→CTA)  mdtK → multidrug efflux pump MdtK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,743,6480GA97.3% 120.1 / ‑4.3 37L64L (CTG→CTA) mdtKmultidrug efflux pump MdtK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (20/16);  minor base T (0/1);  total (20/17)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTTCTCATTATGCTGGTGCTGTGGAATGC  >  NC_000913/1743514‑1743782
                                                                                                                                      |                                                                                                                                      
ctcGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGActactgct                                                                                                                                   <  1:447702/140‑1 (MQ=255)
    cgcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCa                                                                                                                               >  2:81237/1‑140 (MQ=255)
         aTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAAc                                                                                                                          >  1:546817/1‑140 (MQ=255)
                 aaCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTa                                                                                                                  >  1:583510/1‑140 (MQ=255)
                    tGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATcg                                                                                                               <  2:131633/140‑1 (MQ=255)
                     gCGATGGGTTTTGTCGATACCGTGATTGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATcgc                                                                                                              >  1:602014/1‑140 (MQ=255)
                      cGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATcgcg                                                                                                             <  1:39844/140‑1 (MQ=255)
                        aTGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCa                                                                                                           >  2:715822/1‑140 (MQ=255)
                        aTGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCa                                                                                                           >  2:325974/1‑140 (MQ=255)
                         tGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCaa                                                                                                          <  2:546817/140‑1 (MQ=255)
                            gTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATcgcg                                                                                                             <  2:337548/134‑1 (MQ=255)
                            gTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATcgcg                                                                                                             >  1:337548/1‑134 (MQ=255)
                             ttttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTaa                                                                                                      <  2:73542/140‑1 (MQ=255)
                                tGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATg                                                                                                   >  2:324217/1‑140 (MQ=255)
                                     aTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTTTCGGGACTTCTATCTGGGTTCCGGGGATCCTCTTTGGTCACGGGCTACTGCTGGCATTAAAGCCGGGTATCGCGCAAATAAATGGTTcc                                                                                              >  2:203489/1‑140 (MQ=255)
                                                        cTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTg                                                                           >  1:155246/1‑140 (MQ=255)
                                                                  aCCGACATGGCGGCGGTCGCTATCGGAACTTCTATCTGGCTTCCGGGGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGt                                                                 >  2:525043/1‑140 (MQ=255)
                                                                        aTGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTTCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACa                                                           <  1:417004/140‑1 (MQ=255)
                                                                          ggcggcGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACa                                                           >  2:403837/1‑138 (MQ=255)
                                                                          ggcggcGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACa                                                           <  1:403837/138‑1 (MQ=255)
                                                                                 gTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTctg                                                  >  1:507189/1‑140 (MQ=255)
                                                                                 gTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTctg                                                  >  2:425847/1‑140 (MQ=255)
                                                                                                  tATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTG‑‑‑GGCAGGTTTTGTTTCCg                              >  1:527603/1‑140 (MQ=255)
                                                                                                    tCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCTGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTcc                               >  1:321664/1‑140 (MQ=255)
                                                                                                      tGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGt                             <  2:803718/140‑1 (MQ=255)
                                                                                                      tGGCTTCCGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGt                             >  1:606191/1‑140 (MQ=255)
                                                                                                        gCTTCCGGGGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTtc                           <  1:525043/140‑1 (MQ=255)
                                                                                                            ccGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTTCTCAt                       <  1:715822/140‑1 (MQ=255)
                                                                                                            ccGGCGATCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTTCTCAt                       <  2:361486/140‑1 (MQ=255)
                                                                                                                  aTCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTTCTCATTATGCt                 >  1:682560/1‑140 (MQ=255)
                                                                                                                   tCCTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTTCTCATTATGCTg                <  1:196092/140‑1 (MQ=255)
                                                                                                                    ccTCTTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTTCTCATTATGCTgg               >  2:174371/1‑140 (MQ=255)
                                                                                                                      tctTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTTCTCATTATGCTGGTg             <  2:402470/140‑1 (MQ=255)
                                                                                                                      tctTTGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTTCTCATTATGCTGGTg             <  2:379056/140‑1 (MQ=255)
                                                                                                                        tttGGTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTTCTCATTATGCTGGTGCt           >  2:153882/1‑140 (MQ=255)
                                                                                                                           ggTCACGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCTGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTTCTCATTATGCTGGTGCtgtg        <  2:321664/140‑1 (MQ=255)
                                                                                                                               aCGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTTCTCATTATGCTGGTGCTGTGGAAt    <  2:606191/140‑1 (MQ=255)
                                                                                                                                cGGACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTTCTCATTATGCTGGTGCTGTGGAATg   >  2:355303/1‑140 (MQ=255)
                                                                                                                                 ggACTACTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTTCTCATTATGCTGGTGCTGTGGAATGc  <  1:355303/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTTCTCATTATGCTGGTGCTGTGGAATGC  >  NC_000913/1743514‑1743782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑