Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,777,538	A→G	T115A (ACG→GCG)	ydiO →	putative acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,777,538	0	A	G	100.0%	133.0 / NA	40	T115A (ACG→GCG)	ydiO	putative acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/18);  total (22/18)

CTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAACGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTAAAAACGGCAAGGTCTACATCAACGGACAAAAAACCTTTATTACCGGTG  >  NC_000913/1777409‑1777670                                                                                                                                  |                                                                                                                                     cTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATcc                                                                                                                            <  1:122030/140‑1 (MQ=255)    gcgcTGATGGAAGTGTC‑AAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGcc                                                                                                                        >  2:225354/1‑140 (MQ=255)      gcTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCt                                                                                                                       <  1:746750/140‑1 (MQ=255)                     aaaaTGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCg                                                                                                            >  2:205470/1‑136 (MQ=255)                     aaaaTGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCg                                                                                                            <  1:205470/136‑1 (MQ=255)                           cGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAAc                                                                                                  >  2:629870/1‑140 (MQ=255)                             gTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCa                                                                                                >  1:346126/1‑140 (MQ=255)                             gTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCa                                                                                                >  1:593045/1‑140 (MQ=255)                                 tCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTg                                                                                            <  1:225354/140‑1 (MQ=255)                                     gCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTgg                                                                                        >  2:233408/1‑140 (MQ=255)                                       aTTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGAc                                                                                                       >  1:225955/1‑123 (MQ=255)                                       aTTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGAc                                                                                                       <  2:225955/123‑1 (MQ=255)                                                         tCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCa                                                                    >  2:613673/1‑140 (MQ=255)                                                          cAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTCATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCAc                                                                   <  2:741391/140‑1 (MQ=255)                                                             tGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGt                                                                        <  2:71277/132‑1 (MQ=255)                                                             tGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGt                                                                        >  1:71277/1‑132 (MQ=255)                                                                  tCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGt                                                                        <  2:493961/127‑1 (MQ=255)                                                                  tCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGt                                                                        >  1:493961/1‑127 (MQ=255)                                                                   cacaGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTa                                                          <  2:346126/140‑1 (MQ=255)                                                                      aGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCAc                                                                   <  1:63123/128‑1 (MQ=255)                                                                      aGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCAc                                                                   >  2:63123/1‑128 (MQ=255)                                                                       gTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACAcg                                                      >  2:88285/1‑140 (MQ=255)                                                                       gTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACAcg                                                      >  2:522650/1‑140 (MQ=255)                                                                       gTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACAcg                                                      >  1:656497/1‑140 (MQ=255)                                                                           gcgcCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTa                                                  >  1:702522/1‑140 (MQ=255)                                                                           gcgcCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTa                                                  >  1:668673/1‑140 (MQ=255)                                                                           gcgcCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTa                                                  >  1:582975/1‑140 (MQ=255)                                                                             gcCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTaaa                                                >  1:632262/1‑140 (MQ=255)                                                                                   tCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAATGCCACTACCACTTACACGCGTAAAAACGGc                                          <  1:233408/140‑1 (MQ=255)                                                                                    cGGTTCTGCAGATCAGCTACGTAAAACGGCAGAGACCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTAAAAACGGCa                                         <  2:295783/140‑1 (MQ=255)                                                                                    cGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGTTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTAAAAACGGCa                                         <  1:629870/140‑1 (MQ=255)                                                                                    cGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTAAAAACGGCa                                         <  1:613673/140‑1 (MQ=255)                                                                                    cGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTAAAAACGGCa                                         <  2:632262/140‑1 (MQ=255)                                                                                    cGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTAAAAACGGCa                                         <  2:656497/140‑1 (MQ=255)                                                                                           gCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTAAAAACGGCAAGGTCTa                                  >  2:286960/1‑140 (MQ=255)                                                                                                         tAAAACGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTAAAAACGGCAAGGTCTACATCAACGGACaaa                    >  1:753927/1‑140 (MQ=255)                                                                                                           aaaCGGCAGAGAGCACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTAAAAACGGCAAGGTCTACATCAACGGACaaaaa                  >  1:171466/1‑140 (MQ=255)                                                                                                                        cACCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTAAAAACGGCAAGGTCTACATCAACGGACAAAAAACCTTTATTACCg     <  1:522650/140‑1 (MQ=255)                                                                                                                         aCCCTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTAAAAACGGCAAGGTCTACATCAACGGACAAAAAACCTTTATTACCgg    >  1:51166/1‑140 (MQ=255)                                                                                                                           ccTGGAAGCGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTAAAAACGGCAAGGTCTACATCAACGGACAAAAAACCTTTATTACCGGTg  <  2:582975/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     CTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAACGGCAGAGAGCACCCTGGAAACGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTAAAAACGGCAAGGTCTACATCAACGGACAAAAAACCTTTATTACCGGTG  >  NC_000913/1777409‑1777670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑