Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,830,377	T→C	D130G (GAC→GGC)	astA ←	arginine N‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,830,377	0	T	C	100.0%	107.8 / NA	32	D130G (GAC→GGC)	astA	arginine N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/19);  total (13/19)

AGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTGAACCAACGTGCCGACGCGATAGTT  >  NC_000913/1830240‑1830504                                                                                                                                          |                                                                                                                                aGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGccc                                                                                                                               >  2:9715/1‑140 (MQ=255)      ggTGAATAGCCGTGTTCGTCAATCCCCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTc                                                                                                                          <  1:703476/140‑1 (MQ=255)         gAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCACAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCgag                                                                                                                       >  2:168966/1‑140 (MQ=255)               ccGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAg                                                                                                                 >  1:419383/1‑140 (MQ=255)                cGTTTTCCTCATTCACCCCGCGCATTTCAGCAACCACTTTATCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGc                                                                                                                <  2:763482/140‑1 (MQ=255)                     tCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGca                                                                                                           <  1:9715/140‑1 (MQ=255)                         cAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGc                                                                                                       >  2:114893/1‑140 (MQ=255)                              aCCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCgct                                                                                                  >  2:89923/1‑140 (MQ=255)                                                            ttAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGAt                                                                                    >  1:447647/1‑124 (MQ=255)                                                            ttAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGAt                                                                                    <  2:447647/124‑1 (MQ=255)                                                               aaCTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGt                                                                 <  1:89923/140‑1 (MQ=255)                                                                   tGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCTCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGc                                                             >  2:656602/1‑140 (MQ=255)                                                                   tGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGGCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGc                                                             >  2:377546/1‑140 (MQ=255)                                                                     tCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCaa                                                           >  1:520134/1‑140 (MQ=255)                                                                     tCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCa                                                            <  1:202833/139‑1 (MQ=255)                                                                     tCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCa                                                            >  2:202833/1‑139 (MQ=255)                                                                      cgcgAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAAt                                                          <  2:419383/140‑1 (MQ=255)                                                                              gccgccATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTtata                                                  <  2:394062/140‑1 (MQ=255)                                                                               ccgccATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAAt                                                          <  2:246294/131‑1 (MQ=255)                                                                               ccgccATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAAt                                                          >  1:246294/1‑131 (MQ=255)                                                                               ccgccATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAg                                                 <  2:143350/140‑1 (MQ=255)                                                                               ccgccATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGCGTGATCGTTACTGAGAAACAGCGTCGGCAAt                                                          >  1:575956/1‑131 (MQ=255)                                                                               ccgccATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGCGTGATCGTTACTGAGAAACAGCGTCGGCAAt                                                          <  2:575956/131‑1 (MQ=255)                                                                                       aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGGCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAg                                         <  1:377546/140‑1 (MQ=255)                                                                                       aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAg                                         >  1:690646/1‑140 (MQ=255)                                                                                                cgcgATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGct                                       >  1:711746/1‑133 (MQ=255)                                                                                                cgcgATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGct                                       <  2:711746/133‑1 (MQ=255)                                                                                                                       gTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTGAACCAACGTGCCGAcgc         <  2:520134/140‑1 (MQ=255)                                                                                                                       gTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTGAACCAACGTGCCGAcgc         <  1:168966/140‑1 (MQ=255)                                                                                                                       gTTGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTGAACCAACGTGCCGAcgc         <  1:740263/140‑1 (MQ=255)                                                                                                                        ttGCCCTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTGAACCAACGTGCCGAcgcg        <  1:214517/140‑1 (MQ=255)                                                                                                                            ccTCTTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTGAACCAACGTGCCGACGCGATAg    <  2:690978/140‑1 (MQ=255)                                                                                                                              tctTTGCGCCAGCCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTGAACCAACGTGCCGACGCGATAGtt  <  2:690646/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                AGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTGAACCAACGTGCCGACGCGATAGTT  >  NC_000913/1830240‑1830504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑