Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,837,853	T→C	G214G (GGT→GGC)	ynjC →	putative ABC transporter membrane subunit YnjC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,837,853	0	T	C	100.0%	106.4 / NA	31	G214G (GGT→GGC)	ynjC	putative ABC transporter membrane subunit YnjC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/16);  total (15/16)

GCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTAT  >  NC_000913/1837718‑1837982                                                                                                                                        |                                                                                                                                  gCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATc                                                                                                                               >  1:91210/1‑140 (MQ=255)          cAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGc                                                                                                                      <  1:304074/140‑1 (MQ=255)          cAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGc                                                                                                                      <  1:745103/140‑1 (MQ=255)                       tGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAg                                                                                                         >  2:713924/1‑140 (MQ=255)                             aaCTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTTGCa                                                                                                   >  2:381219/1‑140 (MQ=255)                             aaCTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCa                                                                                                   <  2:244329/140‑1 (MQ=255)                             aaCTGGCTGCTGTTGCCCTCCGTCGCACCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCa                                                                                                   <  1:401501/140‑1 (MQ=255)                                  gctgctGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCa                                                                                                   <  2:715432/135‑1 (MQ=255)                                  gctgctGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCa                                                                                                   >  1:715432/1‑135 (MQ=255)                                  gctgctGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGt                                                                                              <  1:552231/140‑1 (MQ=255)                                  gctgctGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGt                                                                                              >  2:788803/1‑140 (MQ=255)                                    tgctgTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCa                                                                                                   <  1:247369/133‑1 (MQ=255)                                    tgctgTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCa                                                                                                   >  2:247369/1‑133 (MQ=255)                                            cccTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAggg                                                                                    >  2:59771/1‑140 (MQ=255)                                                ccGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGAc                                                                                >  1:674114/1‑140 (MQ=255)                                                                 gCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAAcaaacaaa                                                               >  1:448595/1‑140 (MQ=255)                                                                      ggCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGgcg                                                          >  2:84916/1‑140 (MQ=255)                                                                                 cGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGc                                               <  1:65263/140‑1 (MQ=255)                                                                                       ttgCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCctgctg                                         >  2:461746/1‑140 (MQ=255)                                                                                         gCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGtt                                       <  1:788803/140‑1 (MQ=255)                                                                                           cTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGa                                     <  1:84916/140‑1 (MQ=255)                                                                                           cTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGa                                     <  2:448595/140‑1 (MQ=255)                                                                                           cTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGa                                     <  1:59771/140‑1 (MQ=255)                                                                                             ggTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATg                                   <  2:213352/140‑1 (MQ=255)                                                                                                      cggtcgTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTc                          <  2:91210/140‑1 (MQ=255)                                                                                                             ggATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTCGCCGCCt                   >  2:760329/1‑140 (MQ=255)                                                                                                               aTGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTCGCCGCCTAc                 >  1:417283/1‑140 (MQ=255)                                                                                                                  tgGCAATTATGCTCGGGCCAGGCAATCCCCCGACGCTGGCGGGAATTAGCTGGCAGTGGTTAACCCAGGGCGACAGTGACCAACAAACAAAAGGCGCGCTTGCCAACCTGCTGTTTATGCGGTTACCCACCGCCTACGtt              >  2:824505/1‑140 (MQ=255)                                                                                                                   ggCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTCGCCGCCTACGttt             >  2:336516/1‑140 (MQ=255)                                                                                                                         tattCTCGGGCCAGGCAATCCCCCGACG‑TTGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTCGCCGCCTACGTTTTGCTGAg      <  2:456443/140‑1 (MQ=255)                                                                                                                            tctcGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCt    <  1:18703/140‑1 (MQ=255)                                                                                                                              tcGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTAt  <  1:824505/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  GCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTAT  >  NC_000913/1837718‑1837982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑