Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,841,011	T→C	A131A (GCA→GCG)	ynjF ←	putative phosphatidyl transferase, inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,841,011	0	T	C	100.0%	173.1 / NA	50	A131A (GCA→GCG)	ynjF	putative phosphatidyl transferase, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/22);  total (28/22)

GCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGA  >  NC_000913/1840880‑1841145                                                                                                                                    |                                                                                                                                       gcaaaccaTGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCaaa                                                                                                                                >  2:238888/1‑140 (MQ=255)    aaccaTGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGc                                                                                                                             <  2:667435/140‑1 (MQ=255)                             gCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATa                                                                                                    <  2:711304/140‑1 (MQ=255)                                  aaCAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGc                                                                                               <  2:294983/140‑1 (MQ=255)                                   aCAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGc                                                                                               <  1:302954/139‑1 (MQ=255)                                   aCAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGc                                                                                               >  2:302954/1‑139 (MQ=255)                                   aCAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCa                                                                                              <  2:283773/140‑1 (MQ=255)                                        aaGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAaacaa                                                                                         >  2:554433/1‑140 (MQ=255)                                             cGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAAcc                                                                                    >  1:777328/1‑140 (MQ=255)                                               tttCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAg                                                                                  >  2:678987/1‑140 (MQ=255)                                               tttCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAg                                                                                  >  1:501531/1‑140 (MQ=255)                                                    gtgCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAgccgcc                                                                             <  2:501531/140‑1 (MQ=255)                                                     tgCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCt                                                                            >  2:71870/1‑140 (MQ=255)                                                      gCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTg                                                                           <  1:71119/140‑1 (MQ=255)                                                          tCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTg                                                                           >  1:800453/1‑136 (MQ=255)                                                          tCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTg                                                                           <  2:800453/136‑1 (MQ=255)                                                          tCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAg                                                                       >  2:351599/1‑140 (MQ=255)                                                           cGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAgc                                                                      <  2:777328/140‑1 (MQ=255)                                                               tAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCAt                                                                  >  1:404855/1‑140 (MQ=255)                                                                  tCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCAtttt                                                               >  2:551921/1‑140 (MQ=255)                                                                      cccAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGc                                                                          >  1:656323/1‑125 (MQ=255)                                                                      cccAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGc                                                                          <  2:656323/125‑1 (MQ=255)                                                                        cAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCg                                                         >  2:97217/1‑140 (MQ=255)                                                                          gATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGt                                                       <  1:551921/140‑1 (MQ=255)                                                                          gATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGt                                                       <  2:404855/140‑1 (MQ=255)                                                                                     gACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAAt                                            <  1:713173/140‑1 (MQ=255)                                                                                       cTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATaa                                          >  2:448830/1‑140 (MQ=255)                                                                                         tATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTgc                                                     <  2:822756/127‑1 (MQ=255)                                                                                         tATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTgc                                                     >  1:822756/1‑127 (MQ=255)                                                                                         tATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTg                                                      >  1:803712/1‑126 (MQ=255)                                                                                         tATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTg                                                      <  2:803712/126‑1 (MQ=255)                                                                                                 tAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGc                                       <  2:258417/133‑1 (MQ=255)                                                                                                 tAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGc                                       >  1:258417/1‑133 (MQ=255)                                                                                                  aaCCGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGc                               >  1:435438/1‑140 (MQ=255)                                                                                                     cGGGGTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCAcc                            >  2:7327/1‑140 (MQ=255)                                                                                                        ggTTATCAATCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAgc                         <  2:220488/140‑1 (MQ=255)                                                                                                            aTCAACCTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGt                     >  1:614899/1‑140 (MQ=255)                                                                                                                  cTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAaga               >  1:371391/1‑140 (MQ=255)                                                                                                                  cTGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAaga               >  1:318481/1‑140 (MQ=255)                                                                                                                   tGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAgc                         <  2:272925/129‑1 (MQ=255)                                                                                                                   tGATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAgc                         >  1:272925/1‑129 (MQ=255)                                                                                                                    gATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCa                           >  2:675131/1‑126 (MQ=255)                                                                                                                    gATGTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCa                           <  1:675131/126‑1 (MQ=255)                                                                                                                       gTTTTGCTGCCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGaaaa          >  1:511955/1‑140 (MQ=255)                                                                                                                           tgctgcCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCga      <  2:371391/140‑1 (MQ=255)                                                                                                                           tgctgcCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCga      >  1:121786/1‑140 (MQ=255)                                                                                                                           tgctgcCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCga      >  1:205164/1‑140 (MQ=255)                                                                                                                             ctgcCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCgaga    >  2:481837/1‑140 (MQ=255)                                                                                                                              tgcCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCgagag   <  2:511955/140‑1 (MQ=255)                                                                                                                               gcCAGCGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCgagaga  <  2:205164/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       GCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGA  >  NC_000913/1840880‑1841145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑