Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,911,616	T→C	G447G (GGT→GGC)	yebQ →	putative transporter YebQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,911,616	0	T	C	100.0%	128.0 / NA	37	G447G (GGT→GGC)	yebQ	putative transporter YebQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/19);  total (18/19)

CTCGTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGTTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGT  >  NC_000913/1911486‑1911750                                                                                                                                   |                                                                                                                                       cTCGTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGTTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATc                                                                                                                               >  2:621933/1‑140 (MQ=255) cTCGTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATc                                                                                                                               >  2:535808/1‑140 (MQ=255) cTCGTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATc                                                                                                                               >  2:181372/1‑140 (MQ=255) cTCGTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATc                                                                                                                               >  1:82829/1‑140 (MQ=255) cTCGTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATc                                                                                                                               >  1:678123/1‑140 (MQ=255)    gTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACt                                                                                                                            <  2:82829/140‑1 (MQ=255)    gTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACt                                                                                                                            <  2:493014/140‑1 (MQ=255)    gTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACt                                                                                                                            <  1:607737/140‑1 (MQ=255)         cTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGcc                                                                                                                       <  1:181372/140‑1 (MQ=255)                  agTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAg                                                                                                              <  2:326675/140‑1 (MQ=255)                       cggcgcggcgCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCAt                                                                                                         >  2:819868/1‑140 (MQ=255)                       cggcgcggcgCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCAt                                                                                                         >  1:719811/1‑140 (MQ=255)                            cggcgcTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCa                                                                                                                      >  2:31177/1‑122 (MQ=255)                            cggcgcTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCa                                                                                                                      <  1:31177/122‑1 (MQ=255)                            cggcgcTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATaaaaa                                                                                                    >  1:516764/1‑140 (MQ=255)                              gcgcTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATaaaaaaa                                                                                                  <  1:819868/140‑1 (MQ=255)                                  tggtggCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAgcgc                                                                                              <  2:516764/140‑1 (MQ=255)                                                         tttGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAg                                                                       >  2:610958/1‑140 (MQ=255)                                                           tGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTa                                                                     >  2:569496/1‑140 (MQ=255)                                                           tGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTa                                                                     >  2:335502/1‑140 (MQ=255)                                                             gagaTAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTAtt                                                                   <  2:678123/140‑1 (MQ=255)                                                               gaTAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTa                                                                 <  1:610958/140‑1 (MQ=255)                                                                 tAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACt                                                               >  1:410315/1‑140 (MQ=255)                                                                      gTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCata                                                          <  1:621933/140‑1 (MQ=255)                                                                       tatacacGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCatat                                                         <  1:562664/137‑1 (MQ=255)                                                                         cacacGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTa                                                       >  2:532203/1‑140 (MQ=255)                                                                           cacGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACt                                                     >  2:225932/1‑140 (MQ=255)                                                                             cGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTc                                                   <  1:225932/140‑1 (MQ=255)                                                                               tctcGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAg                                                 >  1:209390/1‑140 (MQ=255)                                                                                ctcGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACt                                                               <  2:441607/125‑1 (MQ=255)                                                                                ctcGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACt                                                               >  1:441607/1‑125 (MQ=255)                                                                                      gATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCa                                          <  2:209390/140‑1 (MQ=255)                                                                                            tGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTa                                    >  2:565811/1‑140 (MQ=255)                                                                                                gCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCa                                <  1:535808/140‑1 (MQ=255)                                                                                                         gCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCaa                       <  2:316147/140‑1 (MQ=255)                                                                                                         gCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCaa                       <  2:719811/140‑1 (MQ=255)                                                                                                                        tgtgTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAgcg        >  2:771621/1‑140 (MQ=255)                                                                                                                              aGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCgggt  <  1:565811/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       CTCGTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGTTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGT  >  NC_000913/1911486‑1911750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑