Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	265,136	A→G	V24A (GTC→GCC)	yafX ←	CP4‑6 prophage; protein YafX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	265,136	0	A	G	100.0%	196.0 / NA	57	V24A (GTC→GCC)	yafX	CP4‑6 prophage; protein YafX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/27);  total (30/27)

TAGAACGACCAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGACCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATGGGGATATAAA  >  NC_000913/265001‑265264                                                                                                                                        |                                                                                                                                 tAGAACGACCAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCgg                                                                                                                              >  2:13204/1‑140 (MQ=255)   gAACGACCAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCg                                                                                                                            >  1:647220/1‑140 (MQ=255)     aCGACCAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTg                                                                                                                          >  2:164183/1‑140 (MQ=255)     aCGACCAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTg                                                                                                                          <  1:746075/140‑1 (MQ=255)       gACCAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGAc                                                                                                                        >  1:448243/1‑140 (MQ=255)        aCCAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACg                                                                                                                       >  1:96790/1‑140 (MQ=255)        aCCAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACCCTGTTCATCGGGGGCCGGCGTGACg                                                                                                                       >  2:586565/1‑140 (MQ=255)          cAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGt                                                                                                                     <  1:692228/140‑1 (MQ=255)                 caccGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCa                                                                                                              >  2:317418/1‑140 (MQ=255)                    cGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAAt                                                                                                           <  1:39792/140‑1 (MQ=255)                        gTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCgg                                                                                                       <  2:448243/140‑1 (MQ=255)                         tAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCggg                                                                                                      >  1:212656/1‑140 (MQ=255)                                cacaGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTa                                                                                               >  1:125645/1‑140 (MQ=255)                                 acaGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAg                                                                                              <  2:125645/140‑1 (MQ=255)                                     aaGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGc                                                                                             >  2:322345/1‑137 (MQ=255)                                     aaGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGc                                                                                             <  1:322345/137‑1 (MQ=255)                                      aGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCgg                                                                                                       >  1:743009/1‑126 (MQ=255)                                      aGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAATAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCGGTTCAAATTCgg                                                                                                       <  2:743009/126‑1 (MQ=255)                                         ggTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCg                                                                                      <  2:742976/140‑1 (MQ=255)                                                         gATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTATTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCt                                                                      >  1:380647/1‑140 (MQ=255)                                                         gATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGtgctg                                                                           <  1:576024/135‑1 (MQ=255)                                                         gATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGtgctg                                                                           >  2:576024/1‑135 (MQ=255)                                                              gCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCtgtg                                                                   <  1:56653/138‑1 (MQ=255)                                                              gCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCtgtg                                                                   >  2:56653/1‑138 (MQ=255)                                                                 gCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTATTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTc                                                              <  2:380647/140‑1 (MQ=255)                                                                 gCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTc                                                              <  1:773936/140‑1 (MQ=255)                                                                    ccAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGt                                                                        >  2:267822/1‑127 (MQ=255)                                                                    ccAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGt                                                                        <  1:267822/127‑1 (MQ=255)                                                                       ggAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGt                                                        >  2:32638/1‑140 (MQ=255)                                                                            aGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGtc                                                   >  1:143476/1‑140 (MQ=255)                                                                            aGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGtc                                                   >  1:428883/1‑140 (MQ=255)                                                                             gCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGtgttg                                                                <  2:684239/126‑1 (MQ=255)                                                                             gCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGtgttg                                                                >  1:684239/1‑126 (MQ=255)                                                                                            cgcCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACg                                   >  2:663508/1‑140 (MQ=255)                                                                                               cAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTTAATAAAAACGCCa                                <  1:625548/140‑1 (MQ=255)                                                                                               cAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCa                                <  1:317418/140‑1 (MQ=255)                                                                                               cAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCAGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCa                                <  1:162668/140‑1 (MQ=255)                                                                                                      cTGTGGGCAGAAATCGCAACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGa                         >  2:19561/1‑140 (MQ=255)                                                                                                      cTGTGGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGa                         >  2:799259/1‑140 (MQ=255)                                                                                                       tgtgGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGCCAGTTCAAATTTGGGTAGGTTAGCGGGCGCCAGCTCGTGCTGAGTCTGTGTTGACATGGGTATGTCTCCGTCAATAAAAACGCGAGAGGCGAt                        >  2:725627/1‑140 (MQ=255)                                                                                                        gtgGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGAtg                       <  1:13204/140‑1 (MQ=255)                                                                                                         tgGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGAtgg                      <  1:164183/140‑1 (MQ=255)                                                                                                         tgGCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGAtgg                      <  1:32638/140‑1 (MQ=255)                                                                                                           gCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGAtggct                    >  2:369290/1‑140 (MQ=255)                                                                                                           gCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGAtggct                    >  1:167798/1‑140 (MQ=255)                                                                                                           gCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGAtggct                    >  2:39196/1‑140 (MQ=255)                                                                                                           gCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGAtgg                      <  2:481934/138‑1 (MQ=255)                                                                                                           gCCAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGAtgg                      >  1:481934/1‑138 (MQ=255)                                                                                                            ccAGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGAtggctg                   <  1:829809/140‑1 (MQ=255)                                                                                                              aGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTGTGTCTCCGTCAATAAAAACGCCAGCGGCGAtggctggc                 >  2:451604/1‑140 (MQ=255)                                                                                                              aGAAATCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGAtggctggc                 >  1:695983/1‑140 (MQ=255)                                                                                                                 aaTCGATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTa              <  1:725627/140‑1 (MQ=255)                                                                                                                     gATACGCTGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATggg          <  2:143476/140‑1 (MQ=255)                                                                                                                            tGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGAtggc                     >  1:254395/1‑122 (MQ=255)                                                                                                                            tGTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGAtggc                     <  2:254395/122‑1 (MQ=255)                                                                                                                             gTTCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATGGGGATATaaa  <  2:212656/140‑1 (MQ=255)                                                                                                                              ttCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATg            <  2:60291/129‑1 (MQ=255)                                                                                                                              ttCATCGGGGGCCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATg            >  1:60291/1‑129 (MQ=255)                                                                                                                                        |                                                                                                                                 TAGAACGACCAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGACCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATGGGGATATAAA  >  NC_000913/265001‑265264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑