Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,923,526	T→C	T61A (ACC→GCC)	yebY ←	DUF2511 domain‑containing protein YebY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,923,526	0	T	C	100.0%	179.4 / NA	52	T61A (ACC→GCC)	yebY	DUF2511 domain‑containing protein YebY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/26);  total (26/26)

AACGGTGCCAGACTCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGCGCCGCTG  >  NC_000913/1923393‑1923660                                                                                                                                      |                                                                                                                                       aaCGGTGCCAGACTCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCgg                                                                                                                                  <  2:372070/140‑1 (MQ=255) aaCGGTGCCAGACTCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCgg                                                                                                                                  <  2:227553/140‑1 (MQ=255)     gTGCCAGACTCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATta                                                                                                                              >  2:177212/1‑140 (MQ=255)     gTGCCAGACTCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTATGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATta                                                                                                                              >  2:770010/1‑140 (MQ=255)       gCCAGACTCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATtaat                                                                                                                            >  2:672968/1‑140 (MQ=255)       gCCAGACTCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATtaat                                                                                                                            >  2:339515/1‑140 (MQ=255)         cAGACTCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATtaataa                                                                                                                          <  1:644582/140‑1 (MQ=255)           gACTCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACa                                                                                                                        >  1:54860/1‑140 (MQ=255)             cTCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATa                                                                                                                      >  1:43237/1‑140 (MQ=255)              tCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATtaata                                                                                                                           <  2:655824/134‑1 (MQ=255)              tCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATtaata                                                                                                                           >  1:655824/1‑134 (MQ=255)               cATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACa                                                                                                                    <  1:6087/140‑1 (MQ=255)                aTTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACaa                                                                                                                   >  1:161534/1‑140 (MQ=255)                aTTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACaa                                                                                                                   >  2:565072/1‑140 (MQ=255)                aTTTTTTCGCCGGGATTGTTAGGATCATCAATATGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACaa                                                                                                                   >  1:302955/1‑140 (MQ=255)                                tGTTAGGATCATCAATATGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAGCATACAAAGCATTACCCGGTCgg                                                                                                   <  2:302955/140‑1 (MQ=255)                                    aGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAg                                                                                               <  2:392117/140‑1 (MQ=255)                                      gATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTc                                                                                             <  2:678032/140‑1 (MQ=255)                                      gATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTc                                                                                             <  1:338055/140‑1 (MQ=255)                                          atcaATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCa                                                                                         >  2:776594/1‑140 (MQ=255)                                           tcaATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTc                                                                                             >  1:528328/1‑135 (MQ=255)                                           tcaATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTc                                                                                             <  2:528328/135‑1 (MQ=255)                                           tcaATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCAt                                                                                        <  2:54860/140‑1 (MQ=255)                                               tCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCAcc                                                                                    >  2:329466/1‑140 (MQ=255)                                                         cGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGAt                                                                          >  1:804294/1‑140 (MQ=255)                                                         cGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGAt                                                                          >  2:12528/1‑140 (MQ=255)                                                            aaTGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTaa                                                                       >  2:175832/1‑140 (MQ=255)                                                                  ttGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGgcc                                                                 >  2:717574/1‑140 (MQ=255)                                                                  ttGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGccc                                                                 >  1:534412/1‑140 (MQ=255)                                                                     ggCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCAtt                                                              >  2:776902/1‑140 (MQ=255)                                                                     ggCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCAtt                                                              <  1:12528/140‑1 (MQ=255)                                                                     ggCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCAtt                                                              <  1:672968/140‑1 (MQ=255)                                                                          tGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCt                                                         >  2:391556/1‑140 (MQ=255)                                                                           ggTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCtt                                                        >  1:583336/1‑140 (MQ=255)                                                                                tCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTAccc                                                   >  2:251318/1‑140 (MQ=255)                                                                                 cccACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCAtt                                                              >  1:51123/1‑128 (MQ=255)                                                                                 cccACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCAtt                                                              <  2:51123/128‑1 (MQ=255)                                                                                  ccACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCGAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCAc                                                 <  2:521374/140‑1 (MQ=255)                                                                                    aCTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTAcc                                                    >  1:811767/1‑135 (MQ=255)                                                                                    aCTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTAcc                                                    <  2:811767/135‑1 (MQ=255)                                                                                         cAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCaaaa                                          <  2:583336/140‑1 (MQ=255)                                                                                            cTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGa                                       >  1:312815/1‑140 (MQ=255)                                                                                                cTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGa                                   <  2:534412/140‑1 (MQ=255)                                                                                                            atCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCt                       <  1:177212/140‑1 (MQ=255)                                                                                                            atCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATCTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCt                       <  1:776594/140‑1 (MQ=255)                                                                                                             tCGTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTg                      <  2:804294/140‑1 (MQ=255)                                                                                                               gTTTAAAGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCg                    <  1:770010/140‑1 (MQ=255)                                                                                                                    aaGGATACTGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGca               <  1:251318/140‑1 (MQ=255)                                                                                                                            tGCACGAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAgcgc       <  2:400049/140‑1 (MQ=255)                                                                                                                                 gAGGGCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGCGCCGCTg  <  1:448024/140‑1 (MQ=255)                                                                                                                                   gggCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGcagccag          >  1:822245/1‑130 (MQ=255)                                                                                                                                   gggCACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGcagccag          <  2:822245/130‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       AACGGTGCCAGACTCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGCGCCGCTG  >  NC_000913/1923393‑1923660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑