Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,940,521	T→C	Q372R (CAG→CGG)	mepM ←	peptidoglycan DD‑endopeptidase MepM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,940,521	0	T	C	100.0%	168.5 / NA	49	Q372R (CAG→CGG)	mepM	peptidoglycan DD‑endopeptidase MepM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/25);  total (24/25)

CCCTTCGGTACGCGGCAGTTTTGCCGTCAGCGGGTTTACGGCCTGCTGGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCA  >  NC_000913/1940385‑1940656                                                                                                                                         |                                                                                                                                        cccTTCGGTACGCGGCAGTTTTGCCGTCAGCGGGTTTACGGCCTGCTGGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTc                                                                                                                                      >  1:67964/1‑140 (MQ=255) cccTTCGGTACGCGGCAGTTTTGCCGTCAGCGGGTTTACGGCCTGCTGGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTc                                                                                                                                      >  1:638361/1‑140 (MQ=255) cccTTCGGTACGCGGCAGTTTTGCCGTCAGCGGGTTTACGGCCTGCTGGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTc                                                                                                                                      <  2:371816/140‑1 (MQ=255)      cGGTACGCGGCAGTTTTGCCGTCAGCGGGTTTACGGCCTGCTGGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGt                                                                                                                                 >  2:266312/1‑140 (MQ=255)      cGGTACGCGGCAGTTTTGCCGTCAGCGGGTTTACGGCCTGCTGGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGt                                                                                                                                 <  1:400725/140‑1 (MQ=255)                  gTTTTGCCGTCAGCGGGTTTACGGCCTGCTGGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATc                                                                                                                     <  1:333775/140‑1 (MQ=255)                   ttttGCCGTCAGCGGGTTTACGGCCTGCTGGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCt                                                                                                                    >  1:17171/1‑140 (MQ=255)                   ttttGCCGTCAGCGGGTTTACGGCCTGCTGGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCt                                                                                                                    >  1:138256/1‑140 (MQ=255)                      tGCCGTCAGCGGGTTTACGGCCTGCTGGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTgc                                                                                                                 <  2:17171/140‑1 (MQ=255)                       gCCGTCAGCGGGTTTACGGCCTGCTGGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTgcg                                                                                                                <  2:638361/140‑1 (MQ=255)                         cGTCAGCGGGTTTACGGCCTGCTGGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCa                                                                                                              <  1:169730/140‑1 (MQ=255)                            cAGCGGGTTTACGGCCTGCTGGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGt                                                                                                           >  2:350742/1‑140 (MQ=255)                                gggTTTACGGCCTGCTGGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCat                                                                                                       <  2:254345/140‑1 (MQ=255)                                        ggCCTGCTGGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAAcgcg                                                                                               <  1:266312/140‑1 (MQ=255)                                           ctgctgGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCgtgg                                                                                            >  2:631824/1‑140 (MQ=255)                                                   ttATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTg                                                                                    <  1:791970/140‑1 (MQ=255)                                                      tCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCg                                                                                  >  1:557717/1‑139 (MQ=255)                                                      tCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCg                                                                                  <  2:557717/139‑1 (MQ=255)                                                            cTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCTGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCgtggt                                                                                           >  1:91317/1‑124 (MQ=255)                                                            cTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCTGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCgtggt                                                                                           <  2:91317/124‑1 (MQ=255)                                                                aTAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGa                                                                                 >  1:379899/1‑130 (MQ=255)                                                                aTAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGa                                                                                 <  2:379899/130‑1 (MQ=255)                                                                     gCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGcc                                                                  <  1:721851/140‑1 (MQ=255)                                                                     gCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGcc                                                                  <  1:350742/140‑1 (MQ=255)                                                                     gCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGcc                                                                  <  2:138256/140‑1 (MQ=255)                                                                      cAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCca                                                                 >  1:659443/1‑140 (MQ=255)                                                                        gATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCcaca                                                               <  1:373852/140‑1 (MQ=255)                                                                         aTGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACg                                                                         <  1:683539/129‑1 (MQ=255)                                                                         aTGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACg                                                                         >  2:683539/1‑129 (MQ=255)                                                                         aTGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACat                                                              >  1:532057/1‑140 (MQ=255)                                                                         aTGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACat                                                              >  1:69722/1‑140 (MQ=255)                                                                              gCCCGGTTGAACGTCCGGTATTACCGGAAATCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACAtaataa                                                         >  2:477980/1‑140 (MQ=255)                                                                              gCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACAtaataa                                                         >  2:146438/1‑140 (MQ=255)                                                                              gCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACAtaataa                                                         >  2:445512/1‑140 (MQ=255)                                                                                  ggTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACctg                                                     <  1:308692/140‑1 (MQ=255)                                                                                   gTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACctgc                                                    >  2:575394/1‑140 (MQ=255)                                                                                                          aaGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAacca                             >  2:13439/1‑140 (MQ=255)                                                                                                           aGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAaccac                            <  1:575394/140‑1 (MQ=255)                                                                                                           aGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAaccac                            <  2:69722/140‑1 (MQ=255)                                                                                                           aGCGCGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAAAAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAaccac                            <  2:67964/140‑1 (MQ=255)                                                                                                            gcgcGATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAaccacc                           <  1:584317/140‑1 (MQ=255)                                                                                                             cgcgATACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAAccacca                          >  2:715245/1‑140 (MQ=255)                                                                                                                  tACGGTCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCa                     >  1:811389/1‑140 (MQ=255)                                                                                                                       tCGCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTc                >  2:581255/1‑140 (MQ=255)                                                                                                                         gCCACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCAc              <  2:145422/140‑1 (MQ=255)                                                                                                                          ccACGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCAcc             >  1:650349/1‑140 (MQ=255)                                                                                                                            aCGTTTCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCa           <  1:581255/140‑1 (MQ=255)                                                                                                                                 tCACCTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGaa      >  1:82546/1‑140 (MQ=255)                                                                                                                                     cTTCCGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCa  <  2:811389/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CCCTTCGGTACGCGGCAGTTTTGCCGTCAGCGGGTTTACGGCCTGCTGGTTTATCCATACTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCA  >  NC_000913/1940385‑1940656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑