Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,948,807	G→A	N572N (AAC→AAT)	aspS ←	aspartate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,948,807	0	G	A	100.0%	107.0 / NA	32	N572N (AAC→AAT)	aspS	aspartate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/14);  total (18/14)

AGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCG  >  NC_000913/1948674‑1948943                                                                                                                                      |                                                                                                                                         aGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCa                                                                                                                                    <  1:457441/140‑1 (MQ=255)              cATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCtt                                                                                                                       <  1:200073/140‑1 (MQ=255)                      ttCACTCAGTTGCCGCCTCTATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGtcatc                                                                                                               >  1:454028/1‑140 (MQ=255)                      ttCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGtcatc                                                                                                               >  2:294716/1‑140 (MQ=255)                      ttCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGtcatc                                                                                                               >  1:437082/1‑140 (MQ=255)                      ttCACTAAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGtcatc                                                                                                               >  2:173454/1‑140 (MQ=255)                         aCTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGa                                                                                                            >  2:522494/1‑140 (MQ=255)                                      cTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGccgccg                                                                                               <  2:437082/140‑1 (MQ=255)                                                cgcgAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCgg                                                                                     >  1:604838/1‑140 (MQ=255)                                                  cgAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGTTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGa                                                                                   >  2:678717/1‑140 (MQ=255)                                                          ttCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATa                                                                           <  1:173454/140‑1 (MQ=255)                                                                 aTTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCAc                                                                    >  1:357581/1‑140 (MQ=255)                                                                 aTTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCAc                                                                    <  1:815324/140‑1 (MQ=255)                                                                            atCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCg                                                         >  2:552501/1‑140 (MQ=255)                                                                                  tATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCg                                                   >  1:645675/1‑140 (MQ=255)                                                                                    ttCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGt                                                 >  1:282562/1‑140 (MQ=255)                                                                                    ttCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGt                                                 <  1:294716/140‑1 (MQ=255)                                                                                    ttCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGt                                                 <  2:272173/140‑1 (MQ=255)                                                                                     tctcAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTc                                                >  2:332255/1‑140 (MQ=255)                                                                                      ctcAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTca                                               <  1:355041/140‑1 (MQ=255)                                                                                         aGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTcagca                                            <  2:357581/140‑1 (MQ=255)                                                                                          gCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTcagcag                                           >  1:132408/1‑140 (MQ=255)                                                                                                   acaACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAg                                  <  1:332255/140‑1 (MQ=255)                                                                                                     aaCCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGAc                                >  2:626498/1‑140 (MQ=255)                                                                                                          gAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCa                           >  2:286334/1‑140 (MQ=255)                                                                                                                tcagctcagcCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCg                     <  1:678717/140‑1 (MQ=255)                                                                                                                tcagctcagcCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCg                     <  1:518034/140‑1 (MQ=255)                                                                                                                 cagctcagcCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGa                    >  2:151285/1‑140 (MQ=255)                                                                                                                  agctcagcCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGaa                   >  1:198859/1‑140 (MQ=255)                                                                                                                    ctcagcCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATg                 >  1:624148/1‑140 (MQ=255)                                                                                                                                 gTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCtgcgtg    <  1:552501/140‑1 (MQ=255)                                                                                                                                   cGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCtgcgtgcg  <  2:454028/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         AGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCG  >  NC_000913/1948674‑1948943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑