Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,949,996	T→C	Y176C (TAC→TGC)	aspS ←	aspartate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,949,996	0	T	C	100.0%	117.5 / NA	34	Y176C (TAC→TGC)	aspS	aspartate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/14);  total (20/14)

CTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAGGCGCTGAGCCATTTCCGGAC  >  NC_000913/1949865‑1950131                                                                                                                                    |                                                                                                                                        cTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCAcgc                                                                                                                                 <  1:330313/140‑1 (MQ=255)                        aTCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGt                                                                                                          <  1:170537/140‑1 (MQ=255)                            gATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTg                                                                                                            <  2:320053/134‑1 (MQ=255)                            gATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTg                                                                                                            >  1:320053/1‑134 (MQ=255)                                   aCGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGt                                                                                                          <  2:396426/129‑1 (MQ=255)                                   aCGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGt                                                                                                          >  1:396426/1‑129 (MQ=255)                                   aCGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCgg                                                                                                 >  2:93542/1‑138 (MQ=255)                                   aCGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCgg                                                                                                 <  1:93542/138‑1 (MQ=255)                                   aCGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAg                                                                                               >  1:183603/1‑140 (MQ=255)                                   aCGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAg                                                                                               >  1:472612/1‑140 (MQ=255)                                    cGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACAAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGt                                                                                              >  1:293155/1‑140 (MQ=255)                                       tCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTc                                                                                           >  2:127938/1‑140 (MQ=255)                                                    tcatcaGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAg                                                                              <  1:127938/140‑1 (MQ=255)                                                    tcatcaGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAg                                                                              <  1:30887/140‑1 (MQ=255)                                                     catcaGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGc                                                                             >  1:101222/1‑140 (MQ=255)                                                     catcaGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGc                                                                             >  1:579901/1‑140 (MQ=255)                                                              cTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCAt                                                                    <  2:472612/140‑1 (MQ=255)                                                                gTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTc                                                                      >  1:65471/1‑136 (MQ=255)                                                                gTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTc                                                                      <  2:65471/136‑1 (MQ=255)                                                                gTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATcc                                                                  >  2:576426/1‑140 (MQ=255)                                                                      acaacTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATaaaa                                                            >  1:250751/1‑140 (MQ=255)                                                                         acTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACgg                                                         >  1:636560/1‑140 (MQ=255)                                                                         acTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACgg                                                         >  1:553384/1‑140 (MQ=255)                                                                           tGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGgcg                                                       >  2:520159/1‑140 (MQ=255)                                                                           tGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGgcg                                                       >  2:55942/1‑140 (MQ=255)                                                                                     gCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAgg                                                <  2:766390/137‑1 (MQ=255)                                                                                     gCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAgg                                                >  1:766390/1‑137 (MQ=255)                                                                                              cGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTa                                    >  1:188462/1‑140 (MQ=255)                                                                                               gTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAg                                   <  2:250751/140‑1 (MQ=255)                                                                                                             tgtgCACACGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAgg                     <  1:576426/140‑1 (MQ=255)                                                                                                                     cGAGAAGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAGGCGCTGAGc             <  1:520159/140‑1 (MQ=255)                                                                                                                          aGGCACCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAGGCGCTGAGCCAttt        <  2:183603/140‑1 (MQ=255)                                                                                                                              aCCAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAGGCGCTGAGCCATTTCCgg    >  1:610762/1‑140 (MQ=255)                                                                                                                                cAGGCAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAGGCGCTGAGCCATTTCCGgac  >  2:741939/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        CTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAGGCGCTGAGCCATTTCCGGAC  >  NC_000913/1949865‑1950131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑