Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,014,971	A→G	Q31R (CAG→CGG)	fliG →	flagellar motor switch protein FliG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,014,971	0	A	G	100.0%	125.6 / NA	37	Q31R (CAG→CGG)	fliG	flagellar motor switch protein FliG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/20);  total (17/20)

CGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATT  >  NC_000913/2014837‑2015105                                                                                                                                       |                                                                                                                                       cgcgTGGTGGCGCTGGTCATTCGCCAGTGGATAATTAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTg                                                                                                                                   <  2:160277/140‑1 (MQ=255)  gcgTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGa                                                                                                                                  <  2:43326/140‑1 (MQ=255)         ggCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACaa                                                                                                                           <  1:646465/140‑1 (MQ=255)                 tCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAg                                                                                                                   <  2:813952/140‑1 (MQ=255)                                      aCGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCAc                                                                                              >  1:426652/1‑140 (MQ=255)                                      aCGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTATCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCAc                                                                                              >  2:823226/1‑140 (MQ=255)                                       cGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACg                                                                                             <  1:363602/140‑1 (MQ=255)                                       cGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACg                                                                                             <  1:192032/140‑1 (MQ=255)                                            aTGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGaa                                                                                                    >  2:224335/1‑128 (MQ=255)                                            aTGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGaa                                                                                                    <  1:224335/128‑1 (MQ=255)                                             tGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGAtc                                                                                       >  1:157388/1‑140 (MQ=255)                                                           ggCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCt                                                                         >  1:210452/1‑140 (MQ=255)                                                              aCCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAAc                                                                      >  2:602444/1‑140 (MQ=255)                                                                     aaaGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGAtgtg                                                               <  2:210452/140‑1 (MQ=255)                                                                                      atgaCCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCa                                              >  2:80364/1‑140 (MQ=255)                                                                                        gaCCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCaag                                            >  2:432914/1‑140 (MQ=255)                                                                                         aCCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCaaga                                           >  2:103041/1‑140 (MQ=255)                                                                                         aCCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCaaga                                           <  1:327897/140‑1 (MQ=255)                                                                                           cATTGGCTAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCaagaag                                         <  1:411016/140‑1 (MQ=255)                                                                                             ttGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCt                                       >  2:73458/1‑140 (MQ=255)                                                                                             ttGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCt                                       >  2:105385/1‑140 (MQ=255)                                                                                                        cGGGCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGc                            <  2:495604/140‑1 (MQ=255)                                                                                                         gggCGGCAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGcc                           <  2:220376/140‑1 (MQ=255)                                                                                                          ggcggcAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCg                          <  2:426652/140‑1 (MQ=255)                                                                                                          ggcggcAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCg                          >  1:326529/1‑140 (MQ=255)                                                                                                            cggcAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCa                        <  2:241021/140‑1 (MQ=255)                                                                                                            cggcAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCa                        <  2:453236/140‑1 (MQ=255)                                                                                                            cggcAGAGGTGTTCAAGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCa                        <  1:432914/140‑1 (MQ=255)                                                                                                            cggcAGAGGTGTTCAAGCACCTATCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCa                        <  1:823226/140‑1 (MQ=255)                                                                                                                          aaGCACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGc          <  1:105385/140‑1 (MQ=255)                                                                                                                           aGCACCTCTCCCGGCGTGAAGTACAAACCCTTAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGTTGAACAGTTTGCCGCACTGAATATCAACGcc         <  2:633520/140‑1 (MQ=255)                                                                                                                             cACCTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCaa       >  1:522401/1‑140 (MQ=255)                                                                                                                               ccTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACg     <  2:522401/140‑1 (MQ=255)                                                                                                                               ccTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACg     >  1:365021/1‑140 (MQ=255)                                                                                                                               ccTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACg     >  2:419070/1‑140 (MQ=255)                                                                                                                               ccTCTCCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACg     >  1:675810/1‑140 (MQ=255)                                                                                                                                  ctcCCGGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGAtt  >  2:806983/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       CGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATT  >  NC_000913/2014837‑2015105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑