Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,112,219	T→C	V281V (GTA→GTG)	rfbB ←	dTDP‑glucose 4,6‑dehydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,112,219	0	T	C	100.0%	212.0 / NA	60	V281V (GTA→GTG)	rfbB	dTDP‑glucose 4,6‑dehydratase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/34);  total (26/34)

CCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGTACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTC  >  NC_000913/2112085‑2112353                                                                                                                                       |                                                                                                                                       ccGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAAt                                                                                                                                   <  2:47796/140‑1 (MQ=255)  cGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAAtc                                                                                                                                  <  1:567020/140‑1 (MQ=255)    ctctCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAAtctc                                                                                                                                >  2:677725/1‑140 (MQ=255)         aaaCGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCa                                                                                                                           >  2:111622/1‑140 (MQ=255)           aCGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGc                                                                                                                         <  1:122958/140‑1 (MQ=255)             gTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCaa                                                                                                                       <  2:55004/140‑1 (MQ=255)                 ccTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATca                                                                                                                   >  1:112431/1‑140 (MQ=255)                 ccTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATca                                                                                                                   >  2:799887/1‑140 (MQ=255)                  cTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATcac                                                                                                                  <  2:197696/140‑1 (MQ=255)                    gtgGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACaa                                                                                                                <  2:77909/140‑1 (MQ=255)                       gTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATa                                                                                                             <  1:159491/140‑1 (MQ=255)                         ttCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGt                                                                                                           >  1:556161/1‑140 (MQ=255)                         ttCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGt                                                                                                           <  1:585859/140‑1 (MQ=255)                         ttCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGt                                                                                                           <  2:121784/140‑1 (MQ=255)                          tCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTg                                                                                                          >  1:111157/1‑140 (MQ=255)                            cATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAg                                                                                                        <  1:348985/140‑1 (MQ=255)                            cATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAg                                                                                                        <  2:692371/140‑1 (MQ=255)                                 cAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTa                                                                                                   >  2:110432/1‑140 (MQ=255)                                    tGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACAt                                                                                                >  2:722548/1‑140 (MQ=255)                                      cgcgACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCg                                                                                              <  1:224905/140‑1 (MQ=255)                                                     cAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCg                                                                               >  1:667062/1‑140 (MQ=255)                                                             atagcatagcGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGccc                                                                       >  1:51334/1‑140 (MQ=255)                                                             atagcatagcGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGccc                                                                       >  2:418006/1‑140 (MQ=255)                                                             atagcatagcGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGccc                                                                       >  2:90907/1‑140 (MQ=255)                                                               agcatagcGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCccac                                                                     <  1:665420/140‑1 (MQ=255)                                                                gcatagcGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCccacc                                                                    >  2:425649/1‑140 (MQ=255)                                                                 catagcGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCccacca                                                                   <  2:51334/140‑1 (MQ=255)                                                                    agcGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATg                                                                >  2:629826/1‑140 (MQ=255)                                                                      cggcgATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGtt                                                              >  1:99559/1‑140 (MQ=255)                                                                      cggcgATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGtt                                                              >  1:500269/1‑140 (MQ=255)                                                                      cggcgATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGtt                                                              >  2:218453/1‑140 (MQ=255)                                                                       ggcgATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTta                                                             <  1:677725/140‑1 (MQ=255)                                                                        gcgATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTtat                                                            >  2:443203/1‑140 (MQ=255)                                                                           aTCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCccacc                                                                    >  1:169104/1‑129 (MQ=255)                                                                           aTCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCccacc                                                                    <  2:169104/129‑1 (MQ=255)                                                                           aTCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAg                                                         >  1:800075/1‑140 (MQ=255)                                                                                           tCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTAcc                                         <  1:722548/140‑1 (MQ=255)                                                                                                 aCATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGt                                   <  2:667062/140‑1 (MQ=255)                                                                                                 aCATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGt                                   >  1:462218/1‑140 (MQ=255)                                                                                                   aTAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTa                                 <  1:359569/140‑1 (MQ=255)                                                                                                   aTAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTa                                 <  2:500269/140‑1 (MQ=255)                                                                                                   aTAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTa                                 <  1:630519/140‑1 (MQ=255)                                                                                                   aTAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTa                                 <  1:425649/140‑1 (MQ=255)                                                                                                      aGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTacga                              <  1:218453/140‑1 (MQ=255)                                                                                                      aGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTacga                              <  2:428331/140‑1 (MQ=255)                                                                                                       gTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTacgac                             <  1:629826/140‑1 (MQ=255)                                                                                                       gTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTacgac                             <  2:556161/140‑1 (MQ=255)                                                                                                          aTTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTa                                 <  2:222604/133‑1 (MQ=255)                                                                                                          aTTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTa                                 >  1:222604/1‑133 (MQ=255)                                                                                                            ttGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTacg                               >  1:626497/1‑133 (MQ=255)                                                                                                            ttGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTacg                               <  2:626497/133‑1 (MQ=255)                                                                                                             tGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTacgacg                            <  2:789818/135‑1 (MQ=255)                                                                                                             tGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTacgacg                            >  1:789818/1‑135 (MQ=255)                                                                                                                          aTTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCa          <  1:111622/140‑1 (MQ=255)                                                                                                                          aTTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCa          <  2:112431/140‑1 (MQ=255)                                                                                                                            ttCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATg        <  2:809019/140‑1 (MQ=255)                                                                                                                              ctctTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCa          <  2:433179/136‑1 (MQ=255)                                                                                                                              ctctTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCa          >  1:433179/1‑136 (MQ=255)                                                                                                                                ctTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGATGGTATATAACGCACGCGCATGATCt    >  1:480008/1‑140 (MQ=255)                                                                                                                                  ttCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTc  <  2:99559/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       CCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGTACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTC  >  NC_000913/2112085‑2112353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑