Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,139,747	T→C	intergenic (+262/+12)	yegH → / ← asmA	inner membrane protein YegH/putative assembly protein AsmA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,139,747	0	T	C	100.0%	166.2 / NA	49	intergenic (+262/+12)	yegH/asmA	inner membrane protein YegH/putative assembly protein AsmA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/25);  total (24/25)

GCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGA  >  NC_000913/2139612‑2139876                                                                                                                                        |                                                                                                                                  gCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCAcc                                                                                                                               <  2:685851/140‑1 (MQ=255)   ttGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTg                                                                                                                             <  1:781921/140‑1 (MQ=255)     gAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGt                                                                                                                           <  1:349673/140‑1 (MQ=255)       gCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTaa                                                                                                                         >  2:239913/1‑140 (MQ=255)         gTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAAcc                                                                                                                       >  1:235130/1‑140 (MQ=255)                     cGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTcc                                                                                                           >  1:714228/1‑140 (MQ=255)                     cGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTcc                                                                                                           >  2:73867/1‑140 (MQ=255)                        ggAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGc                                                                                                        <  2:714228/140‑1 (MQ=255)                           aCATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAAc                                                                                                     <  2:13043/140‑1 (MQ=255)                                  gTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACAAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTca                                                                                              <  2:648704/140‑1 (MQ=255)                                    gAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTcaca                                                                                            >  1:767744/1‑140 (MQ=255)                                             acccTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCa                                                                                   <  2:767744/138‑1 (MQ=255)                                                    gATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCttt                                                                                       >  1:805608/1‑129 (MQ=255)                                                    gATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCttt                                                                                       <  2:805608/129‑1 (MQ=255)                                                    gATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                            >  1:235033/1‑140 (MQ=255)                                                         tACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCt                                                                       >  2:403216/1‑140 (MQ=255)                                                               ttCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTc                                                                 >  2:114749/1‑140 (MQ=255)                                                                tCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTcc                                                                >  1:76542/1‑140 (MQ=255)                                                                tCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTcc                                                                >  1:462676/1‑140 (MQ=255)                                                                 cAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCg                                                               <  2:76542/140‑1 (MQ=255)                                                                         aaTGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGccc                                                       >  2:41816/1‑140 (MQ=255)                                                                          atgatgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCa                                                      >  1:602827/1‑140 (MQ=255)                                                                               gccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCAt                                                 <  2:636775/140‑1 (MQ=255)                                                                                ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCAtt                                                >  1:219917/1‑140 (MQ=255)                                                                                ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCAtt                                                >  1:635993/1‑140 (MQ=255)                                                                                ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCAtt                                                >  1:592753/1‑140 (MQ=255)                                                                                  gccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCa                                              >  1:538181/1‑140 (MQ=255)                                                                                                aTGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCGCCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCt                                <  2:602827/140‑1 (MQ=255)                                                                                                aTGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCt                                <  1:73867/140‑1 (MQ=255)                                                                                                aTGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCt                                <  2:235130/140‑1 (MQ=255)                                                                                                  gCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTc                              >  1:744603/1‑140 (MQ=255)                                                                                                  gCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTc                              >  1:731687/1‑140 (MQ=255)                                                                                                   cGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCg                             <  1:558472/140‑1 (MQ=255)                                                                                                   cGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCg                             <  1:491330/140‑1 (MQ=255)                                                                                                     aCGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTc                           >  1:223431/1‑140 (MQ=255)                                                                                                     aCGCTTTACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTc                           <  1:403216/140‑1 (MQ=255)                                                                                                          ttACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTa                      <  2:731687/140‑1 (MQ=255)                                                                                                          ttACGCGTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTa                      <  2:744603/140‑1 (MQ=255)                                                                                                                gTCTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGtt                >  2:656903/1‑140 (MQ=255)                                                                                                                  cTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTg              <  2:235033/140‑1 (MQ=255)                                                                                                                  cTTATCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTg              <  1:41816/140‑1 (MQ=255)                                                                                                                     aTCAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcgc           >  2:823874/1‑140 (MQ=255)                                                                                                                       cAGGCCTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAg         <  1:607247/140‑1 (MQ=255)                                                                                                                            cTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACt    <  2:219917/140‑1 (MQ=255)                                                                                                                            cTACAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACt    <  1:239913/140‑1 (MQ=255)                                                                                                                               cAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGa  <  2:582972/139‑1 (MQ=255)                                                                                                                               cAGGACTGGCCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGa  >  1:582972/1‑139 (MQ=255)                                                                                                                               cAGGACTGGCAACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcg            >  2:466547/1‑129 (MQ=255)                                                                                                                               cAGGACTGGCAACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcg            <  1:466547/129‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  GCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGA  >  NC_000913/2139612‑2139876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑