Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,177,101	A→G	V35A (GTA→GCA)	gatY ←	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,177,101	0	A	G	100.0%	121.8 / NA	38	V35A (GTA→GCA)	gatY	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/13);  total (25/13)

TGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCA  >  NC_000913/2176966‑2177235                                                                                                                                        |                                                                                                                                       tGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTgccac                                                                                                                                    <  2:246151/140‑1 (MQ=255)                  gTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCTACCACTTGc                                                                                                                             <  1:209005/130‑1 (MQ=255)                  gTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCTACCACTTGc                                                                                                                             >  2:209005/1‑130 (MQ=255)                         tGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATtgtg                                                                                                            >  1:658690/1‑140 (MQ=255)                           cTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGAGaa                                                                                                          >  2:229981/1‑140 (MQ=255)                             tCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAata                                                                                                        >  1:436474/1‑140 (MQ=255)                                ccATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTACCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTg                                                                                                     >  1:667204/1‑140 (MQ=255)                                                     aCAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAAccg                                                                                <  2:658690/140‑1 (MQ=255)                                                       aGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGGGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGACCCGCATAAccgcc                                                                              <  2:748378/140‑1 (MQ=255)                                                          ttttCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCcgcg                                                                           >  1:362283/1‑140 (MQ=255)                                                          ttttCTGTACCAGCATGAGTAAATGTACCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCcgcg                                                                           <  2:667204/140‑1 (MQ=255)                                                                     aGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCgttgt                                                                <  2:683966/140‑1 (MQ=255)                                                                      gCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCgttgtt                                                               >  2:479056/1‑140 (MQ=255)                                                                          gAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGc                                                           <  2:436474/140‑1 (MQ=255)                                                                           aGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCa                                                          >  2:247467/1‑140 (MQ=255)                                                                                aTGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGTTTTCTGCCCCCACTTGCATCGTTTCGAGATTGTAAATATTGAATGCCGGACCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGc                                                     <  2:435653/140‑1 (MQ=255)                                                                                aTGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGc                                                     >  2:157906/1‑140 (MQ=255)                                                                                     ccAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCCCCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGc                                                           <  1:398097/129‑1 (MQ=255)                                                                                     ccAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGc                                                           >  2:398097/1‑129 (MQ=255)                                                                                     ccAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGt                                                >  2:329567/1‑140 (MQ=255)                                                                                     ccAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGt                                                >  1:222140/1‑140 (MQ=255)                                                                                       aGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCg                                              >  1:293554/1‑140 (MQ=255)                                                                                           gTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCCCCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCAt                                                         <  1:308380/125‑1 (MQ=255)                                                                                           gTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCAt                                                         >  2:308380/1‑125 (MQ=255)                                                                                              ccGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCt                                                    >  2:469662/1‑127 (MQ=255)                                                                                              ccGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCt                                                    <  1:469662/127‑1 (MQ=255)                                                                                                 gCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCCCCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTa                                    <  2:645434/140‑1 (MQ=255)                                                                                                 gCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGAGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTa                                    <  1:229981/140‑1 (MQ=255)                                                                                                      gatgaCCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAttt                               >  1:388280/1‑140 (MQ=255)                                                                                                        ggcccGGCGCATGCAGGTTGGCAGCGGTTTCTGCCCCCACTTGCATCGTTTCGAGATTGTGAATATTGAAGGCCGAACCCGAATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTc                             <  2:362283/137‑1 (MQ=255)                                                                                                          aCCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTa                                    >  2:669568/1‑131 (MQ=255)                                                                                                          aCCGGCGCATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTa                                    <  1:669568/131‑1 (MQ=255)                                                                                                                gcATGCAGGTTGGCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTg                     <  1:329567/140‑1 (MQ=255)                                                                                                                         ttGGCAGTGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCgtttgtt            >  2:16619/1‑140 (MQ=255)                                                                                                                            gCAGCGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTc         >  2:32422/1‑140 (MQ=255)                                                                                                                                cGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAtt     >  2:81735/1‑140 (MQ=255)                                                                                                                                cGGTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAtt     >  1:596802/1‑140 (MQ=255)                                                                                                                                  gTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGa       <  2:138646/136‑1 (MQ=255)                                                                                                                                  gTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGa       >  1:138646/1‑136 (MQ=255)                                                                                                                                  gTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTc   >  1:762986/1‑140 (MQ=255)                                                                                                                                  gTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTc   >  1:419633/1‑140 (MQ=255)                                                                                                                                  gTTTCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTc   >  1:121684/1‑140 (MQ=255)                                                                                                                                   tttCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa  <  2:388280/140‑1 (MQ=255)                                                                                                                                   tttCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa  <  2:121684/140‑1 (MQ=255)                                                                                                                                   tttCTGCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa  <  1:16619/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCA  >  NC_000913/2176966‑2177235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑