Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,236,296 T→C C344C (TGT→TGC)  preA → NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,236,2960TC100.0% 173.5 / NA 50C344C (TGT→TGC) preANAD‑dependent dihydropyrimidine dehydrogenase subunit PreA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/22);  total (28/22)

GGGTTGTCGCATTATCTCGCCGATCAGGGATTTGATTCGCTGCAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGTGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCG  >  NC_000913/2236159‑2236431
                                                                                                                                         |                                                                                                                                       
gggTTGTCGCATTATCTCGCCGATCAGGGATTTGATTCGCTGCAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATgcgt                                                                                                                                       <  2:481258/140‑1 (MQ=255)
    tGTCGCATTATCTCGCCGATCAGGGATTTGATTCGCTGCAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGc                                                                                                                                   <  1:405412/140‑1 (MQ=255)
                        cAGGGATTTGATTCGCTGCAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGa                                                                                                                             >  1:765716/1‑126 (MQ=255)
                        cAGGGATTTGATTCGCTGCAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGa                                                                                                                             <  2:765716/126‑1 (MQ=255)
                                       cTGCAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGAcggcgg                                                                                                >  2:496882/1‑140 (MQ=255)
                                          cAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTacga                                                                                                      <  1:680106/131‑1 (MQ=255)
                                          cAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTacga                                                                                                      >  2:680106/1‑131 (MQ=255)
                                                    tAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGatg                                                                                   >  2:556019/1‑140 (MQ=255)
                                                    tAGGTCTGACGAATAACAATATTGTCCCGGCGGAAAATTTAGACCGCAGTTATATTGTCTAACACCGTATTAATCTTGATAAATGCGTTGGCTGTGGACCTTGTTATCTTTTCTGTTACGCCGGCGGCCACCAGGCGatg                                                                                   >  2:392797/1‑140 (MQ=255)
                                                         cTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGatggaatg                                                                              >  2:704528/1‑140 (MQ=255)
                                                          tGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGatggaatgg                                                                             >  1:820088/1‑140 (MQ=255)
                                                           ggCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTGTATTTCCTGTTACGACGGCGGTCACCAGGCGatggaatgga                                                                            >  1:311660/1‑140 (MQ=255)
                                                           ggCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGatggaatgga                                                                            >  2:599416/1‑140 (MQ=255)
                                                            gCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAg                                                                           >  1:433729/1‑140 (MQ=255)
                                                              gAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCg                                                                         >  2:68902/1‑140 (MQ=255)
                                                                aTAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCgag                                                                       <  1:68902/140‑1 (MQ=255)
                                                                          tGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGca                                                             <  1:599416/140‑1 (MQ=255)
                                                                            tCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGcaca                                                           >  2:287368/1‑140 (MQ=255)
                                                                            tCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGcaca                                                           >  1:87199/1‑140 (MQ=255)
                                                                            tCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGcaca                                                           >  2:783763/1‑140 (MQ=255)
                                                                            tCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGcaca                                                           >  2:819679/1‑140 (MQ=255)
                                                                             cccGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCacac                                                          <  1:704528/140‑1 (MQ=255)
                                                                               cggcggAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTGTATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCg                                                        <  2:311660/140‑1 (MQ=255)
                                                                               cggcggAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCg                                                        <  2:402561/140‑1 (MQ=255)
                                                                               cggcggAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCg                                                        <  2:433729/140‑1 (MQ=255)
                                                                               cggcggAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCg                                                        <  2:87199/140‑1 (MQ=255)
                                                                               cggcggAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCg                                                        <  1:819679/140‑1 (MQ=255)
                                                                               cggcggAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACAGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCg                                                        >  2:663153/1‑140 (MQ=255)
                                                                                 gcggAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCa                                                      >  2:669623/1‑140 (MQ=255)
                                                                                 gcggAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCa                                                      <  1:783763/140‑1 (MQ=255)
                                                                                      aGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCg                                                        >  2:440818/1‑133 (MQ=255)
                                                                                      aGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCg                                                        <  1:440818/133‑1 (MQ=255)
                                                                                        aTTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAAt                                               <  1:287368/140‑1 (MQ=255)
                                                                                          ttAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATAc                                             <  2:820088/140‑1 (MQ=255)
                                                                                              aCCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCgag                                         <  1:392797/140‑1 (MQ=255)
                                                                                                 gCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGaaa                                      >  1:309082/1‑140 (MQ=255)
                                                                                                  cAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAAt                                     >  1:309345/1‑140 (MQ=255)
                                                                                                               tATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGt                          <  1:367386/138‑1 (MQ=255)
                                                                                                               tATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGt                          >  2:367386/1‑138 (MQ=255)
                                                                                                               tATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCt                        >  2:54100/1‑140 (MQ=255)
                                                                                                                aTCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTg                       <  1:54100/140‑1 (MQ=255)
                                                                                                                 tCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGc                      >  1:100421/1‑140 (MQ=255)
                                                                                                                 tCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGc                      >  1:356677/1‑140 (MQ=255)
                                                                                                                 tCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGc                      >  1:829776/1‑140 (MQ=255)
                                                                                                                 tCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGc                      >  1:814020/1‑140 (MQ=255)
                                                                                                                  ccccGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCt                     >  2:169694/1‑140 (MQ=255)
                                                                                                                  ccccGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACAGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCt                     <  1:663153/140‑1 (MQ=255)
                                                                                                                       tATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTtgtg                >  1:728632/1‑140 (MQ=255)
                                                                                                                       tATCAATCTTGATAAATGCGATGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTtgtg                >  2:575511/1‑140 (MQ=255)
                                                                                                                         tCAATCTTGATAAATGCGATGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGt              <  1:575511/140‑1 (MQ=255)
                                                                                                                                     aaTGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCg  <  1:620393/140‑1 (MQ=255)
                                                                                                                                     aaTGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCg  <  2:402406/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GGGTTGTCGCATTATCTCGCCGATCAGGGATTTGATTCGCTGCAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGTGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCG  >  NC_000913/2236159‑2236431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑