| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,249,929 | T→C | G71G (GGT→GGC) | yeiH → | conserved inner membrane protein YeiH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,249,929 | 0 | T | C | 97.9% | 159.1 / ‑4.3 | 48 | G71G (GGT→GGC) | yeiH | conserved inner membrane protein YeiH |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (21/26); total (21/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
TCACCGGGGTCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGTGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTACCTT > NC_000913/2249793‑2250063 | tCACCGGGGTCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACggcggt < 1:118043/140‑1 (MQ=255)tCACCGGGGTCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACggcggt > 1:579318/1‑140 (MQ=255)tCACCGGGGTCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACggcggt > 1:327709/1‑140 (MQ=255) ccGGGGTCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGtgtg < 2:815953/140‑1 (MQ=255) ggggTCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCt < 1:242771/140‑1 (MQ=255) ggTCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGt < 2:696944/140‑1 (MQ=255) tCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGgtgt > 2:50716/1‑133 (MQ=255) tCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGgtgt < 1:50716/133‑1 (MQ=255) gCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGc < 1:564724/140‑1 (MQ=255) ccTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCa < 1:362979/140‑1 (MQ=255) ccTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCa < 2:676479/140‑1 (MQ=255) tgtgGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAg < 2:327709/140‑1 (MQ=255) gtgGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGc < 2:579318/140‑1 (MQ=255) gtgGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGc < 2:706781/140‑1 (MQ=255) ttCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAAtatttat < 1:416854/140‑1 (MQ=255) ttCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAAtatttat > 1:780698/1‑140 (MQ=255) ccATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGTGGTGTGCTGTTTGCCAAGCAATATttatta < 2:325091/140‑1 (MQ=255) aTTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACg > 2:772807/1‑140 (MQ=255) gcgGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTggg > 2:8688/1‑140 (MQ=255) tGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCa > 2:652177/1‑140 (MQ=255) cGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATg > 1:181261/1‑140 (MQ=255) gTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCt < 1:8688/140‑1 (MQ=255) aCCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCCAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGtt > 2:25422/1‑140 (MQ=255) cGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGc > 2:660298/1‑140 (MQ=255) cGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGc > 1:116763/1‑140 (MQ=255) tCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAAtt > 2:109450/1‑140 (MQ=255) gttgGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCg < 1:652177/140‑1 (MQ=255) gggATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGt < 2:116763/140‑1 (MQ=255) gggATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGt < 1:772807/140‑1 (MQ=255) gATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCg > 1:251706/1‑140 (MQ=255) gATGGTTTTAGGCAACACCACCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCg > 2:713832/1‑140 (MQ=255) tGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGt < 2:391233/140‑1 (MQ=255) gTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTAt < 1:715860/140‑1 (MQ=255) tAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGt < 2:181261/140‑1 (MQ=255) aaCACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGat < 1:109450/140‑1 (MQ=255) acacCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGGATCATTCTTTTTGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCATTGGGatc > 2:778758/1‑140 (MQ=255) cacCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTTTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGatca > 1:477124/1‑140 (MQ=255) cacCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGatca > 2:492568/1‑140 (MQ=255) cacCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGatca > 2:213073/1‑140 (MQ=255) cacCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTGTATAGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGatca > 2:672704/1‑140 (MQ=255) aTCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGCATCAGTGGGATCATCATTGAc > 2:598742/1‑140 (MQ=255) ccGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCAtt > 2:284671/1‑135 (MQ=255) ccGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCAtt < 1:284671/135‑1 (MQ=255) ccGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACgt > 2:530841/1‑140 (MQ=255) gCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACgtgt < 1:660298/140‑1 (MQ=255) acaTCTGGAAAAGTTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGTTTCCGTCTGACGTTCTCGCAAATTGCCGATGACGGTATCAGTGGGATCATCATTGACGTGTTg > 1:634624/1‑140 (MQ=255) caTCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGa < 1:530841/140‑1 (MQ=255) tCTGGAAAAGCTGTGACGGCGGTGTGCTTTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACg < 2:477124/140‑1 (MQ=255) tCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACg < 2:380623/140‑1 (MQ=255) gAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACg > 2:285322/1‑136 (MQ=255) gAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACg < 1:285322/136‑1 (MQ=255) gAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGt < 1:557018/140‑1 (MQ=255) gACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTACCtt > 2:676492/1‑140 (MQ=255) | TCACCGGGGTCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGTGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTACCTT > NC_000913/2249793‑2250063 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |