BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,251,878	G→A	C60Y (TGC→TAC)	yeiI →	putative sugar kinase YeiI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,251,878	0	G	A	100.0%	121.0 / NA	36	C60Y (TGC→TAC)	yeiI	putative sugar kinase YeiI
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/18); total (18/18)

AACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCT  >  NC_000913/2251742‑2252007

aaCCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGt                                                                                                                                >  2:223585/1‑140 (MQ=255)

     gCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAgtgg                                                                                                                           <  1:482307/140‑1 (MQ=255)

     gCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAgtgg                                                                                                                           <  2:531927/140‑1 (MQ=255)

        gATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGACGTTCGCGCGATGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGtggtgg                                                                                                                        <  2:443715/140‑1 (MQ=255)

             agcagAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGAca                                                                                                                   <  2:246572/140‑1 (MQ=255)

                   aCGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGAcaatcaat                                                                                                             >  1:407946/1‑140 (MQ=255)

                    cGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGAcaat                                                                                                                 >  2:590624/1‑135 (MQ=255)

                    cGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGAcaat                                                                                                                 <  1:590624/135‑1 (MQ=255)

                            cGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATAtt                                                                                                    >  1:422586/1‑140 (MQ=255)

                              gACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTcg                                                                                                  >  1:483056/1‑140 (MQ=255)

                                     tgcAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATg                                                                                           <  1:255424/140‑1 (MQ=255)

                                                  ttCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGtt                                                                              >  1:235236/1‑140 (MQ=255)

                                                   tCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTa                                                                             <  1:39794/140‑1 (MQ=255)

                                                                   atatTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTtct                                                             <  2:407946/140‑1 (MQ=255)

                                                                            aTTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGt                                                    >  2:326269/1‑140 (MQ=255)

                                                                             tttAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTa                                                   <  2:422586/140‑1 (MQ=255)

                                                                             tttAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTa                                                   <  1:223585/140‑1 (MQ=255)

                                                                              ttAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATcc                                                        >  2:190585/1‑134 (MQ=255)

                                                                              ttAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATcc                                                        <  1:190585/134‑1 (MQ=255)

                                                                               tAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACa                                                 >  2:59628/1‑140 (MQ=255)

                                                                               tAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACa                                                 <  2:483056/140‑1 (MQ=255)

                                                                                   gcgcAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTc                                             >  2:578071/1‑140 (MQ=255)

                                                                                     gcAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACa                                                 >  2:787916/1‑134 (MQ=255)

                                                                                     gcAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACa                                                 <  1:787916/134‑1 (MQ=255)

                                                                                          ggCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTc                                      <  2:383610/140‑1 (MQ=255)

                                                                                                     aGGCAAAGGTTACATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCCGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGgcggcg                           <  2:103775/140‑1 (MQ=255)

                                                                                                                 cATTCTCACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACa               >  2:267284/1‑140 (MQ=255)

                                                                                                                      tcACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGAAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGcc          >  1:573594/1‑140 (MQ=255)

                                                                                                                       cACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACg                    <  1:624522/129‑1 (MQ=255)

                                                                                                                       cACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACg                    >  2:624522/1‑129 (MQ=255)

                                                                                                                       cACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGccc         >  1:756355/1‑140 (MQ=255)

                                                                                                                       cACCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGccc         >  2:517351/1‑140 (MQ=255)

                                                                                                                        aCCGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCca        <  1:578071/140‑1 (MQ=255)

                                                                                                                          cGAGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTCCCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCcaca      <  2:756355/140‑1 (MQ=255)

                                                                                                                            aGCAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAAt    >  1:450420/1‑140 (MQ=255)

                                                                                                                              cAGGAATACTACGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCt  >  1:22458/1‑140 (MQ=255)

AACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCT  >  NC_000913/2251742‑2252007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`