Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,278,414	G→A	intergenic (‑177/+156)	yejG ← / ← bcr	protein YejG/multidrug efflux pump Bcr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,278,414	0	G	A	100.0%	183.2 / NA	52	intergenic (‑177/+156)	yejG/bcr	protein YejG/multidrug efflux pump Bcr
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/23);  total (29/23)

TTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCGTTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCGGGGGAATGATAGATTTGTGGTTGACATGCATCAACAAAGGAAGCCTT  >  NC_000913/2278285‑2278545                                                                                                                                  |                                                                                                                                    tttAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGAt                                                                                                                           <  1:823334/140‑1 (MQ=255)          tcatcaACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCaa                                                                                                                  <  2:698996/140‑1 (MQ=255)          tcatcaACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCaa                                                                                                                  <  1:259883/140‑1 (MQ=255)           catcaACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAAc                                                                                                                 >  1:270042/1‑140 (MQ=255)           catcaACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAAc                                                                                                                 >  1:720134/1‑140 (MQ=255)               aacaaTCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCttt                                                                                                             <  2:720134/140‑1 (MQ=255)                      cGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCttttt                                                                                                           <  2:68255/135‑1 (MQ=255)                      cGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCttttt                                                                                                           >  1:68255/1‑135 (MQ=255)                       gTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTAc                                                                                                     <  2:521915/140‑1 (MQ=255)                        tCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACa                                                                                                    >  1:323241/1‑140 (MQ=255)                                  gATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGAtgtg                                                                                            >  1:115502/1‑138 (MQ=255)                                  gATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGAtgtg                                                                                            <  2:115502/138‑1 (MQ=255)                                   aTCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGTGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACt                                                                                         >  2:727275/1‑140 (MQ=255)                                   aTCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTaa                                                                                                         <  2:83586/124‑1 (MQ=255)                                   aTCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTaa                                                                                                         >  1:83586/1‑124 (MQ=255)                                   aTCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACt                                                                                         >  1:391460/1‑140 (MQ=255)                                   aTCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACt                                                                                         >  1:411952/1‑140 (MQ=255)                                     cGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACttt                                                                                       >  1:517226/1‑140 (MQ=255)                                             ttGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATaa                                                                               >  1:286634/1‑140 (MQ=255)                                              tGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATaaa                                                                              >  2:384609/1‑140 (MQ=255)                                               gATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAAt                                                                             >  2:107298/1‑140 (MQ=255)                                               gATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAAt                                                                             >  1:597228/1‑140 (MQ=255)                                                  gCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGa                                                                          >  2:503046/1‑140 (MQ=255)                                                      cGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGAtttt                                                                      >  2:707331/1‑140 (MQ=255)                                                      cGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGAtttt                                                                      >  1:478620/1‑140 (MQ=255)                                                      cGCCTGCGTGGCCTCTATGGCTCCGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGAtttt                                                                      >  1:123526/1‑140 (MQ=255)                                                         cTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACa                                                                   <  2:411952/140‑1 (MQ=255)                                                         cTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACa                                                                   <  2:396978/140‑1 (MQ=255)                                                          tGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACat                                                                  <  1:373088/140‑1 (MQ=255)                                                              tGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACataaaa                                                              <  1:107298/140‑1 (MQ=255)                                                               ggCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACataaaat                                                             <  2:270042/140‑1 (MQ=255)                                                               ggCCTCTATGGCTCCGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACataaaat                                                             <  2:123526/140‑1 (MQ=255)                                                                gCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACataaaata                                                            <  1:148099/140‑1 (MQ=255)                                                                   tctATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAAc                                                         >  2:59735/1‑140 (MQ=255)                                                                            ctGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCgg                                                <  2:517226/140‑1 (MQ=255)                                                                               aTTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCggggg                                             <  2:671719/140‑1 (MQ=255)                                                                               aTTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCggggg                                             >  1:341626/1‑140 (MQ=255)                                                                               aTTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCggggg                                             >  1:671719/1‑140 (MQ=255)                                                                                          atCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCGGGGGAATGATAGAtt                                  >  2:749638/1‑140 (MQ=255)                                                                                           tCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCGGGGGAATGATAGAttt                                 <  2:355677/140‑1 (MQ=255)                                                                                            cTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCGGGGGAATGATAGATTtg                                <  1:384609/140‑1 (MQ=255)                                                                                              gCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCGGGGGAATGATAGATTtgtg                              >  2:179827/1‑140 (MQ=255)                                                                                              gCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCGGGGGAATGATAGATTtgtg                              >  2:176630/1‑140 (MQ=255)                                                                                               cGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCGGGGGAATGATAGATTtg                                <  1:759822/137‑1 (MQ=255)                                                                                               cGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCGGGGGAATGATAGATTtg                                >  2:759822/1‑137 (MQ=255)                                                                                               cGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCGGGGGAATGATAGATTTGTgg                             >  2:625273/1‑140 (MQ=255)                                                                                               cGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCGGGGGAATGATAGATTTGTgg                             >  1:26075/1‑140 (MQ=255)                                                                                                   cGTGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCGGGGGAATGATAGATTTGTGGTTGa                         >  1:55224/1‑140 (MQ=255)                                                                                                     tGGTCACGTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCGGGGGAATGATAGATTTGTGGTTGACa                       <  1:59735/140‑1 (MQ=255)                                                                                                            gTTTGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCGGGGGAATGATAGATTTGTGGTTGACATGCATca                <  1:503046/140‑1 (MQ=255)                                                                                                               tGCGGGGCTATATGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCGGGGGAATGATAGATTTGTGGTTGACATGCATcaaca             >  2:619035/1‑140 (MQ=255)                                                                                                                          atGTACCCATTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCGGGGGAATGATAGATTTGTGGTTGACATGCATCAACAAAGGAAGCCtt  <  1:176630/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                    TTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCCGTTTCTGCGATCTAACTCAACCTTTTTAACTACAATGATGTGACTTTTTACATAAATTGATTTTACATAAAATAAACATATATCGGGGGAATGATAGATTTGTGGTTGACATGCATCAACAAAGGAAGCCTT  >  NC_000913/2278285‑2278545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑