Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,283,721	T→C	D76G (GAC→GGC)	yejK ←	nucleoid‑associated protein YejK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,283,721	0	T	C	100.0%	169.1 / NA	49	D76G (GAC→GGC)	yejK	nucleoid‑associated protein YejK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/23);  total (26/23)

AACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATGCAGTTCTGCCACCATCTCAACGACGGTTTCTGTCGGT  >  NC_000913/2283588‑2283855                                                                                                                                      |                                                                                                                                       aaCTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCtt                                                                                                                                  <  1:525950/140‑1 (MQ=255)           aGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACg                                                                                                                        >  1:191949/1‑140 (MQ=255)           aGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACg                                                                                                                        >  2:8491/1‑140 (MQ=255)           aGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACg                                                                                                                        <  2:790024/140‑1 (MQ=255)                      cACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCa                                                                                                             >  2:700966/1‑140 (MQ=255)                      cACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCa                                                                                                             >  2:202182/1‑140 (MQ=255)                      cACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCa                                                                                                             <  1:575274/140‑1 (MQ=255)                       aCCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAg                                                                                                            >  2:429691/1‑140 (MQ=255)                       aCCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAg                                                                                                            >  1:708951/1‑140 (MQ=255)                        ccGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGc                                                                                                           >  1:550843/1‑140 (MQ=255)                        ccGCCAGATAGCGATAGTGGCAAAACAGCACGAATCAACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGTCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGc                                                                                                                       >  2:581297/1‑128 (MQ=255)                        ccGCCAGATAGCGATAGTGGCAAAACAGCACGAATCAACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGTCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGc                                                                                                                       <  1:581297/128‑1 (MQ=255)                           ccAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                                                                        >  1:749711/1‑140 (MQ=255)                               aTAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgcg                                                                                                    <  1:202182/140‑1 (MQ=255)                                tAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgcgc                                                                                                   <  2:714197/140‑1 (MQ=255)                                   cGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCa                                                                                                             <  2:828911/127‑1 (MQ=255)                                   cGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCa                                                                                                             >  1:828911/1‑127 (MQ=255)                                   cGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAg                                                                                                <  1:477808/140‑1 (MQ=255)                                        gTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGcc                                                                                                  <  1:367578/133‑1 (MQ=255)                                        gTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGcc                                                                                                  >  2:367578/1‑133 (MQ=255)                                           gCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActct                                                                                        <  2:749711/140‑1 (MQ=255)                                           gCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActct                                                                                        <  1:700966/140‑1 (MQ=255)                                           gCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActct                                                                                        <  2:342807/140‑1 (MQ=255)                                               aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTc                                                                                    <  1:22832/140‑1 (MQ=255)                                                             aCCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGt                                                                      <  2:550843/140‑1 (MQ=255)                                                              ccGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTa                                                                     >  1:511536/1‑140 (MQ=255)                                                                   aGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCtt                                                                >  2:241411/1‑140 (MQ=255)                                                                   aGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCtt                                                                >  1:318513/1‑140 (MQ=255)                                                                   aGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCtt                                                                >  1:46223/1‑140 (MQ=255)                                                                     cAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTa                                                              >  1:438252/1‑140 (MQ=255)                                                                            atatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTgg                                                       <  2:708951/140‑1 (MQ=255)                                                                                 tCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCta                                                  <  2:191949/140‑1 (MQ=255)                                                                                  cGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtat                                                 >  1:207038/1‑140 (MQ=255)                                                                                    cTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGgcg                                                     >  1:274735/1‑134 (MQ=255)                                                                                    cTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGgcg                                                     <  2:274735/134‑1 (MQ=255)                                                                                    cTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAg                                               >  1:482949/1‑140 (MQ=255)                                                                                       aTTCGTCACTCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGAcc                                            <  2:15698/140‑1 (MQ=255)                                                                                             cACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATg                                       >  2:615730/1‑139 (MQ=255)                                                                                             cACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATg                                       <  1:615730/139‑1 (MQ=255)                                                                                                      ggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATGCAGTTCTGcc                             >  2:760084/1‑140 (MQ=255)                                                                                                              gCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATGCAGTTCTGCCACCAtctc                     >  2:746661/1‑140 (MQ=255)                                                                                                              gCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATGCAGTTCTGCCACCAtctc                     >  2:35276/1‑140 (MQ=255)                                                                                                               cggcgggGCTAAATGCCAGGAAGCCCTCTTCCCCCTGACGCTGTAAGCGAAGCGTCTGCGCCAGTTCACTCTCTCCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATGCAGTTCTGCCACCATCTCa                    <  2:333642/138‑1 (MQ=255)                                                                                                                 gcgcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATGCAGTTCTGCCACCATCTCAac                  <  1:746661/140‑1 (MQ=255)                                                                                                                   gcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATGCAGTTCTGCCACCATCTCAacga                <  2:130579/140‑1 (MQ=255)                                                                                                                      gCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATGCAGTTCTGCCACCATCTCa                    >  2:552710/1‑133 (MQ=255)                                                                                                                      gCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATGCAGTTCTGCCACCATCTCa                    <  1:552710/133‑1 (MQ=255)                                                                                                                            tGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATGCAGTTCTGCCACCATCTCAACGACGGTTTCTg       <  1:8491/140‑1 (MQ=255)                                                                                                                                 ggAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATGCAGTTCTGCCACCATCTCAACGACGGTTTCTGTCGGt  >  2:149594/1‑140 (MQ=255)                                                                                                                                 ggAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATGCAGTTCTGCCACCATCTCAACGACGGTTTCTGTCGGt  >  2:765148/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       AACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATGCAGTTCTGCCACCATCTCAACGACGGTTTCTGTCGGT  >  NC_000913/2283588‑2283855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑