Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,305,788	T→C	M323V (ATG→GTG)	mqo ←	malate:quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,305,788	0	T	C	100.0%	180.7 / NA	52	M323V (ATG→GTG)	mqo	malate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/27);  total (25/27)

GGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCGTAGTCTTTCGCTTCCGGAA  >  NC_000913/2305660‑2305913                                                                                                                                 |                                                                                                                              ggtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTgcgc                                                                                                                    >  1:611071/1‑140 (MQ=255)  gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGcc                                                                                                                   >  1:237435/1‑140 (MQ=255)  gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGcc                                                                                                                   >  1:201448/1‑140 (MQ=255)  gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGcc                                                                                                                   >  2:591959/1‑140 (MQ=255)  gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGcc                                                                                                                   >  2:342695/1‑140 (MQ=255)    ggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCaa                                                                                                                 >  2:579404/1‑140 (MQ=255)     gtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAAc                                                                                                                <  2:637637/140‑1 (MQ=255)     gtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAAc                                                                                                                <  2:237435/140‑1 (MQ=255)      tggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACg                                                                                                               >  2:407615/1‑140 (MQ=255)       ggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACgg                                                                                                              <  1:579404/140‑1 (MQ=255)        gAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGa                                                                                                             <  1:407615/140‑1 (MQ=255)         aaCTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGAt                                                                                                            >  1:702634/1‑140 (MQ=255)             cATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCtt                                                                                                        >  2:98569/1‑140 (MQ=255)              aTTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCt                                                                                                         <  2:91303/138‑1 (MQ=255)              aTTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCt                                                                                                         >  1:91303/1‑138 (MQ=255)                   aTCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCtt                                                                                                        >  1:587689/1‑134 (MQ=255)                   aTCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCtt                                                                                                        <  2:587689/134‑1 (MQ=255)                       cacaATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAAc                                                                                              <  1:98569/140‑1 (MQ=255)                        acaATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACTCCGGTGGTGCGCCAACGGATGCTTTACCGTAAAcc                                                                                             >  1:46506/1‑140 (MQ=255)                        acaATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAAcc                                                                                             >  1:393185/1‑140 (MQ=255)                        acaATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAAcc                                                                                             >  2:429681/1‑140 (MQ=255)                              aaCCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGcc                                                                                       >  1:730253/1‑140 (MQ=255)                                 cGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACTCCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    <  2:46506/140‑1 (MQ=255)                                 cGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    >  2:767456/1‑140 (MQ=255)                                 cGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    >  2:51755/1‑140 (MQ=255)                                 cGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    <  2:292463/140‑1 (MQ=255)                                 cGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACACGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    >  2:653486/1‑140 (MQ=255)                                  gTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGAt                                                                                   <  1:566910/140‑1 (MQ=255)                                  gTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGAt                                                                                   <  2:631747/140‑1 (MQ=255)                                   tttttGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    <  2:676520/138‑1 (MQ=255)                                   tttttGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    >  1:468041/1‑138 (MQ=255)                                   tttttGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    <  2:468041/138‑1 (MQ=255)                                   tttttGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    >  1:676520/1‑138 (MQ=255)                                   tttttGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATg                                                                                  <  2:817446/140‑1 (MQ=255)                                    ttttGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATgg                                                                                 >  1:469358/1‑140 (MQ=255)                                            aaTTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAAc                                                                         <  2:469358/140‑1 (MQ=255)                                              tttGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCa                                                                       <  2:730253/140‑1 (MQ=255)                                                            tGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTcc                                                         >  2:444806/1‑140 (MQ=255)                                                             ggCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCg                                                        <  1:591959/140‑1 (MQ=255)                                                             ggCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCg                                                        <  1:51755/140‑1 (MQ=255)                                                             ggCAAATGGCCCAAACAGCACTACACGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCg                                                        <  1:653486/140‑1 (MQ=255)                                                                  aTGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACa                                                   <  2:201448/140‑1 (MQ=255)                                                                      cccAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGa                                               <  1:767456/140‑1 (MQ=255)                                                                                    cgcgTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCAcc                                 >  1:325821/1‑140 (MQ=255)                                                                                                ccAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCg                     <  1:89856/140‑1 (MQ=255)                                                                                                ccAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCg                     <  1:444806/140‑1 (MQ=255)                                                                                                 cAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCGt                    <  1:819332/140‑1 (MQ=255)                                                                                                    aaCGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCGTAGt                 >  2:158207/1‑140 (MQ=255)                                                                                                      cgcgGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCGTAGTCt               >  2:28008/1‑140 (MQ=255)                                                                                                        cgGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCGTAGTCttt             <  2:611071/140‑1 (MQ=255)                                                                                                            tATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCGTAGTCTTTCGCt         <  2:325821/140‑1 (MQ=255)                                                                                                                          aCCTACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCGTAGTCTTTCGCTTCCGGaa  <  2:197447/133‑1 (MQ=255)                                                                                                                                 |                                                                                                                              GGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCGTAGTCTTTCGCTTCCGGAA  >  NC_000913/2305660‑2305913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑