Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,313,600	T→C	V38A (GTA→GCA)	rcsD →	RcsD phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,313,600	0	T	C	100.0%	218.2 / NA	62	V38A (GTA→GCA)	rcsD	RcsD phosphotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/33);  total (29/33)

AGTTTTAACAGGTCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGTACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTC  >  NC_000913/2313464‑2313735                                                                                                                                         |                                                                                                                                         aGTTTTAACAGGTCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGcaca                                                                                                                                       <  2:460864/140‑1 (MQ=255)      tAACAGGTCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAgcg                                                                                                                                  <  2:529148/140‑1 (MQ=255)           ggTCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGtt                                                                                                                             >  1:638856/1‑140 (MQ=255)             tCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAgc                                                                                                                                   <  2:387989/132‑1 (MQ=255)             tCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAgc                                                                                                                                   >  1:387989/1‑132 (MQ=255)             tCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTaa                                                                                                                           >  1:617937/1‑140 (MQ=255)             tCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTaa                                                                                                                           >  1:75452/1‑140 (MQ=255)             tCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTaa                                                                                                                           >  2:533799/1‑140 (MQ=255)                tAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGc                                                                                                                        <  2:242123/140‑1 (MQ=255)                  aaCACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCt                                                                                                                      <  2:463036/140‑1 (MQ=255)                       gAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAgcg                                                                                                                                  <  2:268977/123‑1 (MQ=255)                       gAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAgcg                                                                                                                                  >  1:268977/1‑123 (MQ=255)                       gAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTg                                                                                                                 >  1:303908/1‑140 (MQ=255)                       gAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTg                                                                                                                 <  2:574783/140‑1 (MQ=255)                         aTGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGaa                                                                                                               >  1:356927/1‑140 (MQ=255)                              tCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATa                                                                                                          >  2:41460/1‑140 (MQ=255)                                     gagaCAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCATAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTa                                                                                                   <  2:733449/140‑1 (MQ=255)                                          aaCGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGa                                                                                              <  2:356927/140‑1 (MQ=255)                                             ggCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTg                                                                                           <  1:553206/140‑1 (MQ=255)                                             ggCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTg                                                                                           <  1:821763/140‑1 (MQ=255)                                             ggCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTg                                                                                           <  2:608586/140‑1 (MQ=255)                                              gCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGt                                                                                          <  2:617937/140‑1 (MQ=255)                                                cACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCg                                                                                        >  2:646255/1‑140 (MQ=255)                                                  cGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGAc                                                                                      <  1:164437/140‑1 (MQ=255)                                                    aCCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACAt                                                                                    <  1:653393/140‑1 (MQ=255)                                                       cGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTAc                                                                                 >  1:27631/1‑140 (MQ=255)                                                        gCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTAcc                                                                                <  1:287261/140‑1 (MQ=255)                                                            ttCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATg                                                                            <  1:560823/140‑1 (MQ=255)                                                                      ccGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCaaaa                                                                 >  1:287292/1‑140 (MQ=255)                                                                        ggggAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAgc                                                               <  1:41460/140‑1 (MQ=255)                                                                         gggAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAgcg                                                              <  2:287292/140‑1 (MQ=255)                                                                               aTTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCATATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGa                                                        >  2:644524/1‑140 (MQ=255)                                                                                ttACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAgc                                                               <  2:79738/132‑1 (MQ=255)                                                                                ttACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAgc                                                               >  1:79738/1‑132 (MQ=255)                                                                                 tACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATa                                                      >  2:376842/1‑140 (MQ=255)                                                                                  aCCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATaa                                                     <  2:638856/140‑1 (MQ=255)                                                                                      gCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTgg                                                 <  1:384054/140‑1 (MQ=255)                                                                                        ttcttTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTATCCCAAAAGCGCGTCGATAACTGGc                                                >  1:736408/1‑140 (MQ=255)                                                                                        ttcttTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCg                                               >  1:146059/1‑140 (MQ=255)                                                                                         tcttTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGt                                              <  2:443503/140‑1 (MQ=255)                                                                                            ttttACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTAc                                           >  1:500311/1‑140 (MQ=255)                                                                                                aCTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGa                                       <  1:460353/140‑1 (MQ=255)                                                                                                aCTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGa                                       <  1:452054/140‑1 (MQ=255)                                                                                                  tGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGAcc                                     >  1:703676/1‑140 (MQ=255)                                                                                                    ttGATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTg                                   >  1:730592/1‑140 (MQ=255)                                                                                                      gATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGc                                 <  2:730592/140‑1 (MQ=255)                                                                                                      gATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGc                                 <  2:27631/140‑1 (MQ=255)                                                                                                      gATCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGc                                 <  1:376842/140‑1 (MQ=255)                                                                                                       aTCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGa                                       <  2:704878/133‑1 (MQ=255)                                                                                                       aTCATTGTGTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGa                                       >  1:704878/1‑133 (MQ=255)                                                                                                            tgtgTTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCt                           >  1:118962/1‑140 (MQ=255)                                                                                                              tgtTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTAc                         >  2:630076/1‑140 (MQ=255)                                                                                                              tgtTACTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTAc                         >  1:128677/1‑140 (MQ=255)                                                                                                                   cTGGTGACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGacaa                    >  1:271514/1‑140 (MQ=255)                                                                                                                        gACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGc                                 >  1:137062/1‑122 (MQ=255)                                                                                                                        gACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGc                                 <  2:137062/122‑1 (MQ=255)                                                                                                                        gACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTg               >  1:457901/1‑140 (MQ=255)                                                                                                                        gACGATGGGTGTAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTg               <  2:703676/140‑1 (MQ=255)                                                                                                                                 tgtAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGcgacga      <  1:163738/140‑1 (MQ=255)                                                                                                                                   tAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCATATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGAcc    <  1:644524/140‑1 (MQ=255)                                                                                                                                   tAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGAcc    <  2:271514/140‑1 (MQ=255)                                                                                                                                   tAATGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGAcc    >  1:795251/1‑140 (MQ=255)                                                                                                                                     aTGGCACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTc  >  2:794361/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         AGTTTTAACAGGTCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGTACAAAGCGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTAT‑CCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTC  >  NC_000913/2313464‑2313735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑