Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,324,502	T→C	S132P (TCG→CCG)	atoA →	acetyl‑CoA:acetoacetyl‑CoA transferase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,324,502	0	T	C	100.0%	127.2 / NA	38	S132P (TCG→CCG)	atoA	acetyl‑CoA:acetoacetyl‑CoA transferase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/19);  total (19/19)

ATCCGTGGCGGTCATATTGATGCCTGCGTGCTCGGCGGTTTGCAAGTAGACGAAGAAGCAAACCTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTA  >  NC_000913/2324370‑2324637                                                                                                                                     |                                                                                                                                        aTCCGTGGCGGTCATATTGATGCCTGCGTGCTCGGCGGTTTGCAAGTAGACGAAGAAGCAAACCTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCaa                                                                                                                                  <  2:34660/140‑1 (MQ=255)           gTCATATTGATGCCTGCGTGCTCGGCGGTTTGCAAGTAGACGAAGAAGCAAACCTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGatcatc                                                                                                                        >  1:660443/1‑140 (MQ=255)             cATATTGATGCCTGCGTGCTCGGCGGTTTGCAAGTAGACGAAGAAGCAAACCTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGc                                                                                                                      >  2:1219/1‑140 (MQ=255)                      gCCTGCGTGCTCGGCGGTTTGCAAGTAGACGAAGAAGCAAACCTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACa                                                                                                             >  2:437858/1‑140 (MQ=255)                      gCCTGCGTGCTCGGCGGTTTGCAAGTAGACGAAGAAGCAAACCTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACa                                                                                                             >  1:402789/1‑140 (MQ=255)                       ccTGCGTGCTCGGCGGTTTGCAAGTAGACGAAGAAGCAAACCTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACAt                                                                                                            >  1:316313/1‑140 (MQ=255)                         tGCGTGCTCGGCGGTTTGCAAGTAGACGAAGAAGCAAACCTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTg                                                                                                          >  1:336873/1‑140 (MQ=255)                         tGCGTGCTCGGCGGTTTGCAAGTAGACGAAGAAGCAAACCTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTg                                                                                                          >  1:75800/1‑140 (MQ=255)                           cGTGCTCGGCGGTTTGCAAGTAGACGAAGAAGCAAACCTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTgcg                                                                                                        <  1:251944/140‑1 (MQ=255)                                                     aagaagCAAACCTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTg                                                                              <  2:473006/140‑1 (MQ=255)                                                          gCAAACCTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTCTGcgccg                                                                         <  1:337664/140‑1 (MQ=255)                                                             aaCCTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTg                                                                      <  2:562360/140‑1 (MQ=255)                                                             aaCCTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTg                                                                      <  2:316313/140‑1 (MQ=255)                                                               ccTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCa                                                                    <  1:163430/140‑1 (MQ=255)                                                               ccTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCa                                                                    <  2:402789/140‑1 (MQ=255)                                                                         tGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCcact                                                          <  1:635384/140‑1 (MQ=255)                                                                          gggTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCAcc                                                                  >  2:128551/1‑131 (MQ=255)                                                                          gggTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCAcc                                                                  <  1:128551/131‑1 (MQ=255)                                                                          gggTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCcactc                                                         >  1:201799/1‑140 (MQ=255)                                                                          gggTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCcactc                                                         >  1:785966/1‑140 (MQ=255)                                                                                tGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTgcg                                                   >  2:13784/1‑140 (MQ=255)                                                                                tGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTgcg                                                   >  2:195927/1‑140 (MQ=255)                                                                                tGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTgcg                                                   >  2:592520/1‑140 (MQ=255)                                                                                  ccTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCcactc                                                         <  1:470333/132‑1 (MQ=255)                                                                                  ccTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCcactc                                                         >  2:470333/1‑132 (MQ=255)                                                                                  ccTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCa                                                 >  1:79355/1‑140 (MQ=255)                                                                                  ccTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCa                                                 <  1:437858/140‑1 (MQ=255)                                                                                   cTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCaa                                                >  1:511090/1‑140 (MQ=255)                                                                                       gAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATg                                            <  2:402101/140‑1 (MQ=255)                                                                                       gAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATg                                            <  1:592520/140‑1 (MQ=255)                                                                                           aTGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGt                                        <  1:305755/140‑1 (MQ=255)                                                                                                 cccGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATAATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCatat                                  >  2:145511/1‑140 (MQ=255)                                                                                                  ccGGTATGGGTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATg                                 <  2:201799/140‑1 (MQ=255)                                                                                                         gggTGGCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTa                          <  1:1219/140‑1 (MQ=255)                                                                                                            tGGCGCGATGGATCTGGGGACCGGGGCGCGCAAAGTGAGCATCGCCATGGAACATTGCGCAAAAGATGGTTCAGCAAAAAGTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATTCGGTGCATATGCTGGTTACTg                       <  2:535151/140‑1 (MQ=255)                                                                                                             ggCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGa                      <  2:511090/140‑1 (MQ=255)                                                                                                             ggCGCGATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGa                      <  1:2750/140‑1 (MQ=255)                                                                                                               cgcgATGGATCTGGTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAAc                    >  2:190456/1‑140 (MQ=255)                                                                                                                            gTGACCGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCg       >  2:554356/1‑140 (MQ=255)                                                                                                                                 cGGGCCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTa  >  1:124899/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        ATCCGTGGCGGTCATATTGATGCCTGCGTGCTCGGCGGTTTGCAAGTAGACGAAGAAGCAAACCTCGCGAACTGGGTAGTGCCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTA  >  NC_000913/2324370‑2324637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑