Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	321,642	A→G	D12G (GAC→GGC)	ykgE →	putative lactate utilization oxidoreductase YkgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	321,642	0	A	G	100.0%	183.0 / NA	53	D12G (GAC→GGC)	ykgE	putative lactate utilization oxidoreductase YkgE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/26);  total (27/26)

GCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCG  >  NC_000913/321507‑321772                                                                                                                                        |                                                                                                                                   gCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGcc                                                                                                                                >  2:250144/1‑140 (MQ=255)    aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTg                                                                                                                             >  2:12538/1‑140 (MQ=255)         aTTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATc                                                                                                                        >  2:414780/1‑140 (MQ=255)          ttAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCa                                                                                                                       >  1:461359/1‑140 (MQ=255)          ttAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCa                                                                                                                       >  2:618027/1‑140 (MQ=255)                   aaCGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCa                                                                                                              <  1:250144/140‑1 (MQ=255)                       ccATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACgag                                                                                                          <  2:184407/140‑1 (MQ=255)                        cATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACgaga                                                                                                         <  1:618027/140‑1 (MQ=255)                        cATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACgaga                                                                                                         <  2:701705/140‑1 (MQ=255)                               attGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGtg                                                                                                  <  2:825259/140‑1 (MQ=255)                                  gatgatTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCa                                                                                                              <  1:307284/125‑1 (MQ=255)                                  gatgatTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCa                                                                                                              >  2:307284/1‑125 (MQ=255)                                  gatgatTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGtgctg                                                                                               >  1:697269/1‑140 (MQ=255)                                  gatgatTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGtgctg                                                                                               >  2:295923/1‑140 (MQ=255)                                    tgattgatCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTgctgct                                                                                             <  2:305311/140‑1 (MQ=255)                                      attgatCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc                                                                                           <  1:596013/140‑1 (MQ=255)                                        tgatCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGAc                                                                                                        >  1:353908/1‑125 (MQ=255)                                        tgatCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGAc                                                                                                        <  2:353908/125‑1 (MQ=255)                                             ccccGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGaaaa                                                                                    >  1:780571/1‑140 (MQ=255)                                                cGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACt                                                                                 >  1:684986/1‑140 (MQ=255)                                                cGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACt                                                                                 >  1:113135/1‑140 (MQ=255)                                                     ggggggAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCt                                                                            >  2:388819/1‑140 (MQ=255)                                                       ggggAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGt                                                                          <  1:204664/140‑1 (MQ=255)                                                               aTCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTaaa                                                                  >  1:476461/1‑140 (MQ=255)                                                                 cGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAAtt                                                                >  1:622787/1‑140 (MQ=255)                                                                  gggCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAAttt                                                               >  2:786129/1‑140 (MQ=255)                                                                  gggCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAAttt                                                               >  1:571172/1‑140 (MQ=255)                                                                  gggCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAAttt                                                               >  1:747935/1‑140 (MQ=255)                                                                     cATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAAttt                                                               >  1:483761/1‑137 (MQ=255)                                                                     cATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAAttt                                                               <  2:483761/137‑1 (MQ=255)                                                                      aTAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCg                                                           <  1:12538/140‑1 (MQ=255)                                                                             ggCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAAc                                                    <  2:697269/140‑1 (MQ=255)                                                                                 tGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAggg                                                <  1:388819/140‑1 (MQ=255)                                                                                       aaTGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGAtgctg                                          <  1:295923/140‑1 (MQ=255)                                                                                          ggAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCgg                                       >  2:659666/1‑140 (MQ=255)                                                                                           gAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGt                                      <  2:276717/140‑1 (MQ=255)                                                                                           gAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGt                                      <  2:571172/140‑1 (MQ=255)                                                                                                    ttGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCg                             <  1:491356/140‑1 (MQ=255)                                                                                                      gtgAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGCGACAGGGATCCTGCGGTCAGCCTGCGAt                           >  2:491356/1‑140 (MQ=255)                                                                                                      gtgAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGAtgctg                                          >  1:732470/1‑125 (MQ=255)                                                                                                      gtgAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGAt                           >  1:2818/1‑140 (MQ=255)                                                                                                      gtgAATGTCAATTTCTTCGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCAGCTGTCGCGTAAATTTCCCGGAGAAACAGGGAtgctg                                          <  2:732470/125‑1 (MQ=255)                                                                                                        gAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCt                                >  1:391782/1‑133 (MQ=255)                                                                                                        gAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCt                                <  2:391782/133‑1 (MQ=255)                                                                                                        gAATGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCa                         >  1:677312/1‑140 (MQ=255)                                                                                                          aTGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCTCGAGACGCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAAt                       <  2:461359/140‑1 (MQ=255)                                                                                                          aTGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAAt                       <  1:659666/140‑1 (MQ=255)                                                                                                           tGTCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATa                      <  2:827051/140‑1 (MQ=255)                                                                                                             tCAATTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGc                    >  1:281335/1‑140 (MQ=255)                                                                                                               aaTTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCgg                  <  2:167806/140‑1 (MQ=255)                                                                                                               aaTTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCgg                  <  1:223530/140‑1 (MQ=255)                                                                                                                aTTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGt                 <  2:780571/140‑1 (MQ=255)                                                                                                                aTTTCTTTGTCACCTGTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGt                 <  1:786129/140‑1 (MQ=255)                                                                                                                               gTATTGGTGGCGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCg  >  2:336601/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                   GCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCG  >  NC_000913/321507‑321772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑