Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,401,970	T→C	H28R (CAC→CGC)	nuoE ←	NADH:quinone oxidoreductase subunit E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,401,970	0	T	C	100.0%	243.0 / NA	69	H28R (CAC→CGC)	nuoE	NADH:quinone oxidoreductase subunit E
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/43);  total (26/43)

GCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATAA  >  NC_000913/2401835‑2402100                                                                                                                                        |                                                                                                                                   gCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAgcggtg                                                                                                                                <  1:341874/140‑1 (MQ=255)  ccACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGc                                                                                                                               >  2:794560/1‑140 (MQ=255)          tCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTg                                                                                                                       >  1:644282/1‑140 (MQ=255)          tCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTg                                                                                                                       >  2:22909/1‑140 (MQ=255)          tCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTg                                                                                                                       >  2:504165/1‑140 (MQ=255)          tCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTg                                                                                                                       >  1:260213/1‑140 (MQ=255)               gTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGa                                                                                                                  <  2:644282/140‑1 (MQ=255)                tCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGAt                                                                                                                 <  2:807124/140‑1 (MQ=255)                tCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGAt                                                                                                                 <  1:676665/140‑1 (MQ=255)                  gCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCg                                                                                                               >  1:237381/1‑140 (MQ=255)                  gCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTACTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCg                                                                                                               >  2:112111/1‑140 (MQ=255)                    ttGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCt                                                                                                             <  2:536277/140‑1 (MQ=255)                    ttGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCt                                                                                                             <  1:486319/140‑1 (MQ=255)                     tGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCtt                                                                                                            >  1:399112/1‑140 (MQ=255)                       ccGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCa                                                                                                          <  1:532343/140‑1 (MQ=255)                       ccGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCa                                                                                                          <  1:794762/140‑1 (MQ=255)                       ccGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCa                                                                                                          <  1:498501/140‑1 (MQ=255)                       ccGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCa                                                                                                          <  1:332751/140‑1 (MQ=255)                         ggAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACg                                                                                                        <  2:558668/140‑1 (MQ=255)                           aaTCCCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGct                                                                                                      <  2:561168/140‑1 (MQ=255)                           aaTACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGct                                                                                                      <  2:413308/140‑1 (MQ=255)                           aaTACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGct                                                                                                      <  2:582796/140‑1 (MQ=255)                                  aGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGct                                                                                                      >  2:56359/1‑133 (MQ=255)                                  aGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGct                                                                                                      <  1:56359/133‑1 (MQ=255)                                    cacaTCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGct                                                                                                      >  2:270071/1‑131 (MQ=255)                                    cacaTCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGct                                                                                                      <  1:270071/131‑1 (MQ=255)                                    cacaTCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGcc                                                                                                 <  1:127738/136‑1 (MQ=255)                                    cacaTCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGcc                                                                                                 >  2:127738/1‑136 (MQ=255)                                     acaTCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACt                                                                                            <  2:724789/140‑1 (MQ=255)                                         cGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGc                                                                                        >  2:267902/1‑140 (MQ=255)                                         cGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTACATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGc                                                                                        >  1:162079/1‑140 (MQ=255)                                              aTCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCaaa                                                                                   >  2:253963/1‑140 (MQ=255)                                                   gTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCt                                                                              <  1:504165/140‑1 (MQ=255)                                                   gTGGATCGCACCATCCGGCACCCAGCCACGCTACTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCt                                                                              <  1:112111/140‑1 (MQ=255)                                                      gATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCgg                                                                           <  2:237381/140‑1 (MQ=255)                                                         cGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGttt                                                                        >  1:601021/1‑140 (MQ=255)                                                           cACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTtgt                                                                      >  2:191424/1‑140 (MQ=255)                                                           cACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTtgt                                                                      >  2:613717/1‑140 (MQ=255)                                                           cACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTtgt                                                                      >  2:188087/1‑140 (MQ=255)                                                             ccATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTgg                                                                    <  1:22909/140‑1 (MQ=255)                                                             ccATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTgg                                                                    <  1:267902/140‑1 (MQ=255)                                                                tCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGcc                                                                               >  1:802998/1‑126 (MQ=255)                                                                tCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGcc                                                                               <  2:802998/126‑1 (MQ=255)                                                                 ccGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGgttgt                                                                >  2:239785/1‑140 (MQ=255)                                                                    gCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGa                                                             <  1:253963/140‑1 (MQ=255)                                                                      aCCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGAtt                                                           <  1:191424/140‑1 (MQ=255)                                                                      aCCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGAtt                                                           <  1:239785/140‑1 (MQ=255)                                                                        ccAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATtct                                                         <  1:188087/140‑1 (MQ=255)                                                                        ccAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATtct                                                         <  2:665796/140‑1 (MQ=255)                                                                                     ttCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAg                                            <  2:809752/140‑1 (MQ=255)                                                                                      tCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGc                                           >  2:200540/1‑140 (MQ=255)                                                                                       cTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCg                                          <  1:282065/140‑1 (MQ=255)                                                                                          aaCGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTc                                       <  2:479960/140‑1 (MQ=255)                                                                                              aTTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCcaca                                   >  1:813146/1‑140 (MQ=255)                                                                                                 ttCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCcaca                                   >  2:483089/1‑137 (MQ=255)                                                                                                 ttCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCcaca                                   <  1:483089/137‑1 (MQ=255)                                                                                                     gcgcTTCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACa                            <  1:613717/140‑1 (MQ=255)                                                                                                         ttCAATGGACGCCGCACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAAc                        <  1:307022/140‑1 (MQ=255)                                                                                                                   gccgcACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATa              >  2:323583/1‑140 (MQ=255)                                                                                                                   gccgcACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATa              <  2:260213/140‑1 (MQ=255)                                                                                                                     cgcACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACa                            <  2:98313/124‑1 (MQ=255)                                                                                                                     cgcACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACa                            >  1:98313/1‑124 (MQ=255)                                                                                                                     cgcACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACt            >  1:33617/1‑140 (MQ=255)                                                                                                                      gcACGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTg           <  1:200540/140‑1 (MQ=255)                                                                                                                      gcACGCGGGTCTTCGTAGCGGTACATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTg           <  2:162079/140‑1 (MQ=255)                                                                                                                        aCGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGcc         <  2:601021/140‑1 (MQ=255)                                                                                                                        aCGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGcc         <  1:794560/140‑1 (MQ=255)                                                                                                                        aCGCGGGTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGcc         <  2:813146/140‑1 (MQ=255)                                                                                                                              gTCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATa   <  2:399112/140‑1 (MQ=255)                                                                                                                               tCTTCGTAGCGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATaa  <  1:118415/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   GCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATAA  >  NC_000913/2401835‑2402100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑