Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,492,281	A→G	V325A (GTG→GCG)	frc ←	formyl‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,492,281	0	A	G	100.0%	184.7 / NA	53	V325A (GTG→GCG)	frc	formyl‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/28);  total (25/28)

CGGTCAGATATTTTCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGT  >  NC_000913/2492147‑2492417                                                                                                                                       |                                                                                                                                         cGGTCAGATATTTTCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCt                                                                                                                                     <  1:576868/140‑1 (MQ=255)  ggTCAGATATTTTCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCtt                                                                                                                                    >  2:410308/1‑140 (MQ=255)  ggTCAGATATTTTCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCt                                                                                                                                     >  2:68495/1‑139 (MQ=255)  ggTCAGATATTTTCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCt                                                                                                                                     <  1:68495/139‑1 (MQ=255)   gTCAGATATTTTCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTc                                                                                                                                   >  1:357918/1‑140 (MQ=255)     cAGATATTTTCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCa                                                                                                                                  >  2:500470/1‑139 (MQ=255)     cAGATATTTTCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCa                                                                                                                                  <  1:500470/139‑1 (MQ=255)      aGATATTTTCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATg                                                                                                                                <  2:545370/140‑1 (MQ=255)          atTTTCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTa                                                                                                                            <  2:803584/140‑1 (MQ=255)            tttCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATc                                                                                                                          <  1:483713/140‑1 (MQ=255)             ttCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTa                                                                                                                            <  2:527630/137‑1 (MQ=255)             ttCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTa                                                                                                                            <  2:346511/137‑1 (MQ=255)             ttCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTa                                                                                                                            >  1:346511/1‑137 (MQ=255)             ttCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTa                                                                                                                            >  1:527630/1‑137 (MQ=255)              tCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCaa                                                                                                                        >  2:559631/1‑140 (MQ=255)                 aCGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAg                                                                                                                     <  1:246573/140‑1 (MQ=255)                  cGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGt                                                                                                                    <  2:357918/140‑1 (MQ=255)                  cGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGt                                                                                                                    >  1:748471/1‑140 (MQ=255)                   gCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTg                                                                                                                   <  2:748471/140‑1 (MQ=255)                    cAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGa                                                                                                                  <  1:559631/140‑1 (MQ=255)                     aaCGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGAc                                                                                                                 <  2:51261/140‑1 (MQ=255)                     aaCGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGAc                                                                                                                 <  2:437103/140‑1 (MQ=255)                             ttCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTAtt                                                                                                         >  2:341833/1‑140 (MQ=255)                                              tGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGACGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCaaaa                                                                                        >  1:671412/1‑140 (MQ=255)                                               gCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCaaaaa                                                                                       >  2:681611/1‑140 (MQ=255)                                                    tttGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCg                                                                                  >  1:456645/1‑140 (MQ=255)                                                          gcAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAata                                                                            >  2:230682/1‑140 (MQ=255)                                                          gcAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAata                                                                            >  2:801771/1‑140 (MQ=255)                                                              agagGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATAtgtg                                                                        >  1:778744/1‑140 (MQ=255)                                                               gagGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTgg                                                                       <  2:441399/140‑1 (MQ=255)                                                                   gATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGt                                                                   <  1:341833/140‑1 (MQ=255)                                                                        aGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGc                                                              <  1:188684/140‑1 (MQ=255)                                                                        aGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGc                                                              >  2:795229/1‑140 (MQ=255)                                                                        aGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGc                                                              <  2:676351/140‑1 (MQ=255)                                                                          tGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCAt                                                            >  1:260334/1‑140 (MQ=255)                                                                          tGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCAt                                                            <  1:801771/140‑1 (MQ=255)                                                                          tGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCAt                                                            <  1:230682/140‑1 (MQ=255)                                                                             aaTTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATggg                                                         >  2:591939/1‑140 (MQ=255)                                                                                             aaaaCCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCgg                                         <  1:115811/140‑1 (MQ=255)                                                                                             aaaaCCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCgg                                         >  2:701086/1‑140 (MQ=255)                                                                                               aaCCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGAt                                       <  1:701086/140‑1 (MQ=255)                                                                                                    gTGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTa                                  >  2:308594/1‑140 (MQ=255)                                                                                                     tGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTaa                                 >  2:730302/1‑140 (MQ=255)                                                                                                     tGCACAAGGAATATCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTaa                                 >  1:465084/1‑140 (MQ=255)                                                                                                                 atCAAACTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGtt                     >  1:167913/1‑140 (MQ=255)                                                                                                                      aCTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCg                >  2:344648/1‑140 (MQ=255)                                                                                                                       cTGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGa               <  1:308594/140‑1 (MQ=255)                                                                                                                        tGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGAt              >  2:357569/1‑140 (MQ=255)                                                                                                                        tGAGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGAt              <  2:260334/140‑1 (MQ=255)                                                                                                                          aGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATgg            <  2:598226/140‑1 (MQ=255)                                                                                                                          aGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATgg            <  1:591939/140‑1 (MQ=255)                                                                                                                          aGTCAAATAGGCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATgg            <  2:778744/140‑1 (MQ=255)                                                                                                                                   ggCCGCCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAgg   <  2:167913/140‑1 (MQ=255)                                                                                                                                    gccgccGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGgt  >  2:521787/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         CGGTCAGATATTTTCCACGCAACGGTTGTTCCACTTCAACAACACTGCCACTTTGGCGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGT  >  NC_000913/2492147‑2492417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑