Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,530,088 A→T I30N (ATT→AAT)  ligA ← DNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,530,0880AT89.4% 141.5 / 9.3 47I30N (ATT→AAT) ligADNA ligase
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base T (22/20);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

TGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAA  >  NC_000913/2529953‑2530221
                                                                                                                                       |                                                                                                                                     
tGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTc                                                                                                                                   >  2:92576/1‑140 (MQ=255)
  gCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCg                                                                                                                                 >  2:228076/1‑140 (MQ=255)
   cGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCgg                                                                                                                                >  2:143175/1‑140 (MQ=255)
     tATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGgcg                                                                                                                              <  1:199128/140‑1 (MQ=255)
      aTCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGgcgc                                                                                                                             >  1:28354/1‑140 (MQ=255)
      aTCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGgcgc                                                                                                                             >  2:407467/1‑140 (MQ=255)
      aTCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGgcgc                                                                                                                             >  1:567621/1‑140 (MQ=255)
      aTCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGgc                                                                                                                               >  2:427606/1‑138 (MQ=255)
      aTCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGgc                                                                                                                               <  1:427606/138‑1 (MQ=255)
       tCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGca                                                                                                                            >  2:814209/1‑140 (MQ=255)
          ggCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCA‑‑‑‑‑‑GCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATcaca                                                                                                                   <  1:717863/140‑1 (MQ=255)
             tGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGcatccatc                                                                                                                      <  1:228076/140‑1 (MQ=255)
              gAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATca                                                                                                                     <  2:444623/140‑1 (MQ=255)
               aaaGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATca                                                                                                                     <  2:666662/139‑1 (MQ=255)
               aaaGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATca                                                                                                                     >  1:666662/1‑139 (MQ=255)
                aaGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGTGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATcaca                                                                                                                   <  2:179969/140‑1 (MQ=255)
                 aGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGgcg                                                                                                                              >  1:573223/1‑128 (MQ=255)
                 aGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGgcg                                                                                                                              <  2:573223/128‑1 (MQ=255)
                    cgccAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGAt                                                                                                               <  2:6137/140‑1 (MQ=255)
                    cgccAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGAt                                                                                                               <  2:442330/140‑1 (MQ=255)
                        aGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAg                                                                                                           <  1:814209/140‑1 (MQ=255)
                        aGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAg                                                                                                           <  2:567621/140‑1 (MQ=255)
                        aGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAg                                                                                                           <  2:28354/140‑1 (MQ=255)
                           ggcgCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGata                                                                                                        <  1:143175/140‑1 (MQ=255)
                                                 tAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGtt                                                                                  <  1:250475/140‑1 (MQ=255)
                                                 tAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGtt                                                                                  >  1:336568/1‑140 (MQ=255)
                                                  aGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTc                                                                                 <  2:336568/140‑1 (MQ=255)
                                                     cGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCa                                                                              <  1:53894/140‑1 (MQ=255)
                                                         tCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTc                                                                          >  1:103425/1‑140 (MQ=255)
                                                                       ttCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAgttgttgtt                                                            >  2:366424/1‑140 (MQ=255)
                                                                       ttCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAgttgttgtt                                                            >  1:101226/1‑140 (MQ=255)
                                                                        tCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTc                                                           >  2:371728/1‑140 (MQ=255)
                                                                           ggATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCgat                                                        <  1:407467/140‑1 (MQ=255)
                                                                           ggATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATAATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCgat                                                        <  1:287139/140‑1 (MQ=255)
                                                                                 ttGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTc                                                  >  2:623812/1‑140 (MQ=255)
                                                                                 ttGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTc                                                  >  1:820632/1‑140 (MQ=255)
                                                                                      ttCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCatat                                             <  1:92576/140‑1 (MQ=255)
                                                                                         cAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGc                                          >  2:85658/1‑140 (MQ=255)
                                                                                         cAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGc                                          <  1:57629/140‑1 (MQ=255)
                                                                                          aGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCa                                         >  2:30051/1‑140 (MQ=255)
                                                                                                      tCGCGCATCAGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTa                             >  2:80429/1‑140 (MQ=255)
                                                                                                               aGCCTGTCGTATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCTGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAccccc                    >  2:726422/1‑140 (MQ=255)
                                                                                                                        tATTCAGCGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCgg           <  1:366424/140‑1 (MQ=255)
                                                                                                                               cGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCggg          <  1:386625/134‑1 (MQ=255)
                                                                                                                               cGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCggg          >  2:386625/1‑134 (MQ=255)
                                                                                                                               cGTCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCg    >  2:32532/1‑140 (MQ=255)
                                                                                                                                 tCGGGATTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGaa  <  2:820632/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
TGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAA  >  NC_000913/2529953‑2530221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑