Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,597,353	A→G	V89A (GTT→GCT)	purC ←	phosphoribosylaminoimidazole‑succinocarboxamide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,597,353	0	A	G	100.0%	102.6 / NA	30	V89A (GTT→GCT)	purC	phosphoribosylaminoimidazole‑succinocarboxamide synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/18);  total (12/18)

CGTTGACCATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGA  >  NC_000913/2597219‑2597487                                                                                                                                       |                                                                                                                                       cGTTGACCATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGc                                                                                                                                   <  1:342382/140‑1 (MQ=255)  gTTGACCATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGGTCAGCCCAATACCTTCTTTTCTTTCCAAACGTTTTACCCCAGAGCCCGCAGCACGGGTACGCACGAAACACCCACCCCGcc                                                                                                                                  >  2:88810/1‑139 (MQ=255)                  tGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGGTGGTCTCCATACCCTTTTCGATTTTCCAACGATTTCCCCCAGAGCCACCAGCCCGGGTTGGCGCCACACACTCCGCCCCCACCAAATCCCCCttttt                                                                                                                  >  2:608942/1‑140 (MQ=255)                     aTGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCAc                                                                                                               <  2:18539/140‑1 (MQ=255)                             gtTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACAtt                                                                                                       <  1:176336/140‑1 (MQ=255)                                    aGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATc                                                                                                <  2:352120/140‑1 (MQ=255)                                            aTCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCa                                                                                        <  1:142441/140‑1 (MQ=255)                                            aTCGAACAGCGGCGGGGTCAGCTCAAAACCCTCTTTTATTCCAAAACGTTTCACCAGAGAGCCAGCAGCACGGGTACGCACCACACACCCAACCCGCACCCCATCCAGCCTTTTCTCCACACAATCGGGATCGGagagga                                                                                        >  2:109575/1‑138 (MQ=255)                                                aaCAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACg                                                                                    <  1:109575/140‑1 (MQ=255)                                                         gggTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTg                                                                           <  2:340486/140‑1 (MQ=255)                                                               aGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCgg                                                                     >  1:760077/1‑140 (MQ=255)                                                                         cttcttCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCt                                                           <  1:437597/140‑1 (MQ=255)                                                                           tcttcGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTc                                                         >  1:270097/1‑140 (MQ=255)                                                                           tcttcGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGAAACCCGCTTc                                                         >  1:23129/1‑140 (MQ=255)                                                                              tcGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGc                                                      >  2:532545/1‑140 (MQ=255)                                                                                       aGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCt                                             <  1:100008/140‑1 (MQ=255)                                                                                       aGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCt                                             <  1:439464/140‑1 (MQ=255)                                                                                             ttCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAAt                                       >  1:63438/1‑140 (MQ=255)                                                                                             ttCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAAt                                       >  2:186980/1‑140 (MQ=255)                                                                                              tCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATg                                      <  2:694820/140‑1 (MQ=255)                                                                                                aCCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGAAACCCGCTTCAGCCAGTTTGCTCATAATGaa                                    <  2:23129/140‑1 (MQ=255)                                                                                                       agCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTg                             >  2:666080/1‑140 (MQ=255)                                                                                                          cagcagCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAAc                          >  2:435747/1‑140 (MQ=255)                                                                                                             cagcaCGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACttg                       <  2:227777/140‑1 (MQ=255)                                                                                                                caCGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACttgttg                    <  1:186980/140‑1 (MQ=255)                                                                                                                caCGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACttgttg                    <  1:158150/140‑1 (MQ=255)                                                                                                                  cGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTgttgtt                  <  2:273909/140‑1 (MQ=255)                                                                                                                       aCGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGATCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCa             >  1:124614/1‑140 (MQ=255)                                                                                                                          cACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATAc          >  2:645817/1‑140 (MQ=255)                                                                                                                               cacacTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTg     >  1:327190/1‑140 (MQ=255)                                                                                                                               cacacTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTg     >  2:152764/1‑140 (MQ=255)                                                                                                                                  acTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGa  <  1:435747/140‑1 (MQ=255)                                                                                                                                  acTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGa  <  1:608942/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       CGTTGACCATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGA  >  NC_000913/2597219‑2597487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑