Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,612,137 T→C Y80H (TAT→CAT)  hyfR → DNA‑binding transcriptional activator HyfR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,612,1370TC90.6% 150.8 / 5.4 53Y80H (TAT→CAT) hyfRDNA‑binding transcriptional activator HyfR
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base C (26/22);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCG  >  NC_000913/2612008‑2612273
                                                                                                                                 |                                                                                                                                        
ttattaCGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCga                                                                                                                                <  1:136354/140‑1 (MQ=255)
      cgcgCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCgag                                                                                                                               >  2:617860/1‑135 (MQ=255)
      cgcgCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCgag                                                                                                                               <  1:617860/135‑1 (MQ=255)
        cgcCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGCTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCg                                                                                                                        >  1:68384/1‑140 (MQ=255)
             tCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGAt                                                                                                                             >  2:382691/1‑130 (MQ=255)
             tCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGAt                                                                                                                             <  1:382691/130‑1 (MQ=255)
              cATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGCTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGattatt                                                                                                                  <  2:68384/140‑1 (MQ=255)
                     cTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCa                                                                                                           >  2:756092/1‑140 (MQ=255)
                                           tACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCagtagt                                                                                     >  2:163049/1‑140 (MQ=255)
                                                     tGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCgg                                                                           >  1:540637/1‑140 (MQ=255)
                                                     tGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCATTCTCGGCAGTAGTGGAATGGCgg                                                                           >  2:336405/1‑140 (MQ=255)
                                                         cTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGGATGGCGGAGCt                                                                       <  1:549339/140‑1 (MQ=255)
                                                          tGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTa                                                                      >  2:217844/1‑140 (MQ=255)
                                                          tGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTa                                                                      >  1:603548/1‑140 (MQ=255)
                                                            cATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGTAGTAGTGGAATGGCGGAGCTACg                                                                    >  1:428882/1‑140 (MQ=255)
                                                             aTGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGc                                                                   <  1:203296/140‑1 (MQ=255)
                                                              tGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCt                                                                  >  1:52585/1‑140 (MQ=255)
                                                                 tctcAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGc                                                                        <  2:115880/131‑1 (MQ=255)
                                                                 tctcAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGc                                                                        >  1:115880/1‑131 (MQ=255)
                                                                 tctcAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTa                                                               >  1:105687/1‑140 (MQ=255)
                                                                    cAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTc                                                            >  1:826381/1‑140 (MQ=255)
                                                                    cAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTc                                                            <  2:105687/140‑1 (MQ=255)
                                                                      aaaGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCg                                                                            <  1:282280/122‑1 (MQ=255)
                                                                      aaaGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCg                                                                            >  2:282280/1‑122 (MQ=255)
                                                                            aCTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCAc                                                    >  2:806510/1‑140 (MQ=255)
                                                                             cTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACg                                                   >  2:63639/1‑140 (MQ=255)
                                                                               tCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGc                                                 <  1:799071/140‑1 (MQ=255)
                                                                                   ttACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTc                                             >  2:306130/1‑140 (MQ=255)
                                                                                        gCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGtttt                                        >  1:406474/1‑140 (MQ=255)
                                                                                         cTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTa                                       >  2:354683/1‑140 (MQ=255)
                                                                                         cTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTa                                       <  1:315814/140‑1 (MQ=255)
                                                                                         cTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTa                                       <  1:63639/140‑1 (MQ=255)
                                                                                         cTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTa                                       >  2:578375/1‑140 (MQ=255)
                                                                                         cTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTa                                       <  2:540637/140‑1 (MQ=255)
                                                                                           aCCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGc                                                 >  2:693585/1‑128 (MQ=255)
                                                                                           aCCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGc                                                 <  1:693585/128‑1 (MQ=255)
                                                                                           aCCATGATGATGGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACt                                     >  1:617924/1‑140 (MQ=255)
                                                                                              atgatgatgGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTcc                                  <  2:790124/140‑1 (MQ=255)
                                                                                                 atgatgGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCa                                <  2:50656/139‑1 (MQ=255)
                                                                                                 atgatgGCAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCa                                >  1:50656/1‑139 (MQ=255)
                                                                                                      ggCAGTGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTa                                       >  2:331003/1‑127 (MQ=255)
                                                                                                      ggCAGTGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTa                                       <  1:331003/127‑1 (MQ=255)
                                                                                                        cAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGca                        <  2:603548/140‑1 (MQ=255)
                                                                                                        cAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGca                        <  1:306130/140‑1 (MQ=255)
                                                                                                        cAGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGca                        <  1:163049/140‑1 (MQ=255)
                                                                                                         aGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTa                                       >  2:809345/1‑124 (MQ=255)
                                                                                                         aGCGATGTGACGGCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTa                                       <  1:809345/124‑1 (MQ=255)
                                                                                                                     gCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGca                        <  2:177826/127‑1 (MQ=255)
                                                                                                                     gCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGca                        >  1:177826/1‑127 (MQ=255)
                                                                                                                     gCAACAACAGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAActg           >  2:539073/1‑140 (MQ=255)
                                                                                                                          aacaGAGCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAActggctgg      <  1:806510/140‑1 (MQ=255)
                                                                                                                              gagCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGTAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGcgcg  <  2:428882/140‑1 (MQ=255)
                                                                                                                              gagCATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGcgcg  <  1:756092/140‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                        
TTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCG  >  NC_000913/2612008‑2612273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑