Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	324,899	T→C	T156A (ACA→GCA)	ykgH ←	uncharacterized protein YkgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	324,899	0	T	C	100.0%	204.2 / NA	58	T156A (ACA→GCA)	ykgH	uncharacterized protein YkgH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/32);  total (26/32)

TGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTCAGCAGTTGGTAAAGCT  >  NC_000913/324767‑325035                                                                                                                                     |                                                                                                                                           tGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGc                                                                                                                                     >  2:787984/1‑140 (MQ=255)      aGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACt                                                                                                                                >  2:265169/1‑140 (MQ=255)      aGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACt                                                                                                                                >  2:229052/1‑140 (MQ=255)             aTCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGcata                                                                                                                                         >  1:666991/1‑124 (MQ=255)             aTCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGcata                                                                                                                                         <  2:666991/124‑1 (MQ=255)                 cAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTg                                                                                                                     <  2:48550/140‑1 (MQ=255)                      ccAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGc                                                                                                                <  1:274237/140‑1 (MQ=255)                      ccAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGc                                                                                                                <  1:378390/140‑1 (MQ=255)                         ggCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGtt                                                                                                             >  1:563135/1‑140 (MQ=255)                         ggCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGtt                                                                                                             >  1:489353/1‑140 (MQ=255)                              aTGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑t                                                                                                      >  2:550121/1‑140 (MQ=255)                              aTGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑t                                                                                                      >  2:667377/1‑140 (MQ=255)                               tcgcagcaGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑tc                                                                                                     <  2:563135/138‑1 (MQ=255)                               tGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑tc                                                                                                     >  2:142778/1‑140 (MQ=255)                                ggCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑tct                                                                                                    <  2:508025/140‑1 (MQ=255)                                ggCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑tct                                                                                                    <  2:202992/140‑1 (MQ=255)                                ggCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑tct                                                                                                    <  1:492479/140‑1 (MQ=255)                                    gcagGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATc                                                                                                <  2:51185/140‑1 (MQ=255)                                              tattttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTtgc                                                                                      <  1:20953/140‑1 (MQ=255)                                                 tttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTtgcctg                                                                                   <  2:489353/140‑1 (MQ=255)                                                  ttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTtgcctgc                                                                                  >  1:194538/1‑140 (MQ=255)                                                    ttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCa                                                                                <  2:35624/140‑1 (MQ=255)                                                         tCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATg                                                                           <  1:510371/140‑1 (MQ=255)                                                            ggTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGTCCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGata                                                                        <  2:769681/140‑1 (MQ=255)                                                                 gCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTg                                                                   <  1:615337/140‑1 (MQ=255)                                                                  cTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGa                                                                  >  2:545230/1‑140 (MQ=255)                                                                    tataGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACg                                                                <  1:787984/140‑1 (MQ=255)                                                                        gTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCa                                                              <  1:550121/140‑1 (MQ=255)                                                                           acacAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCa                                                         >  1:456690/1‑140 (MQ=255)                                                                            cacaGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCACCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCAt                                                        <  2:146614/140‑1 (MQ=255)                                                                             acaGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCAtt                                                       <  1:229052/140‑1 (MQ=255)                                                                             acaGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCAtt                                                       <  1:265169/140‑1 (MQ=255)                                                                               aGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTg                                                     >  1:465188/1‑140 (MQ=255)                                                                                gCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTTCCATTATGATATTCTGACGCATACCATTTGa                                                    <  1:533178/140‑1 (MQ=255)                                                                                 ccATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGat                                                   >  2:750931/1‑140 (MQ=255)                                                                                    tCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCAttt                                                      <  2:479965/134‑1 (MQ=255)                                                                                    tCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCAttt                                                      >  1:479965/1‑134 (MQ=255)                                                                                    tCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGataat                                                >  2:261075/1‑140 (MQ=255)                                                                                     cACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGataata                                               <  1:383134/140‑1 (MQ=255)                                                                                             ggATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTg                                       <  1:261075/140‑1 (MQ=255)                                                                                                 aCAATGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCAcg                                   <  1:545230/140‑1 (MQ=255)                                                                                                     tGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCgag                               >  2:11004/1‑140 (MQ=255)                                                                                                     tGACCAGTTCCTGGTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCgag                               >  1:44738/1‑140 (MQ=255)                                                                                                                 ggTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCtt                   >  2:524588/1‑140 (MQ=255)                                                                                                                 ggTCAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCtt                   >  1:559873/1‑140 (MQ=255)                                                                                                                    cAGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTcag                <  1:142778/140‑1 (MQ=255)                                                                                                                     aGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTcagc               >  1:561913/1‑140 (MQ=255)                                                                                                                     aGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTca                 >  2:104565/1‑138 (MQ=255)                                                                                                                     aGGTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTca                 <  1:104565/138‑1 (MQ=255)                                                                                                                      ggTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTcagca              >  1:215803/1‑140 (MQ=255)                                                                                                                      ggTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTcagca              <  2:456690/140‑1 (MQ=255)                                                                                                                      ggTATATCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTcagca              <  1:100656/140‑1 (MQ=255)                                                                                                                        tataTCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTCAGCAGt            <  1:667377/140‑1 (MQ=255)                                                                                                                          tatCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTCAGCAGTTg          >  2:225039/1‑140 (MQ=255)                                                                                                                          tatCATTTTTGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTCAGCAGTTg          >  1:473398/1‑140 (MQ=255)                                                                                                                               tttttGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTCAGCAGTTGGTaaa     <  2:215803/140‑1 (MQ=255)                                                                                                                                 tttGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTCAGCAGTTGGTAAAGc   >  1:720385/1‑140 (MQ=255)                                                                                                                                 tttGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTCAGCAGTTGGTAAAGc   >  1:372509/1‑140 (MQ=255)                                                                                                                                  ttGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTCAGCAGTTGGTAAAGct  <  1:11004/140‑1 (MQ=255)                                                                                                                                  ttGCATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTCAGCAGTTGGTAAAGct  <  1:225039/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                           TGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTA‑‑TCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTCAGCAGTTGGTAAAGCT  >  NC_000913/324767‑325035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑