Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,649,467	G→A	R941C (CGT→TGT)	yfhM ←	alpha2‑macroglobulin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,649,467	0	G	A	100.0%	160.9 / NA	49	R941C (CGT→TGT)	yfhM	alpha2‑macroglobulin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/23);  total (26/23)

TGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAGCACACCACTATCAACGGCA  >  NC_000913/2649330‑2649597                                                                                                                                          |                                                                                                                                   tgtAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACAcc                                                                                                                                  >  1:673331/1‑140 (MQ=255)       cGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGAc                                                                                                                            <  1:145432/140‑1 (MQ=255)           ccTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCCCCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTCACCTTc                                                                                                                        >  2:222006/1‑140 (MQ=255)           ccTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTc                                                                                                                        >  1:448021/1‑140 (MQ=255)             ttCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAACTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCaa                                                                                                                      >  2:240803/1‑140 (MQ=255)              tCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAAt                                                                                                                     >  2:606371/1‑140 (MQ=255)              tCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAAt                                                                                                                     >  1:220001/1‑140 (MQ=255)                    tGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTg                                                                                                               <  2:210655/140‑1 (MQ=255)                      ttCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGAc                                                                                                             >  1:178775/1‑140 (MQ=255)                       tCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGAcc                                                                                                            >  1:499116/1‑140 (MQ=255)                        cGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCg                                                                                                           >  2:20878/1‑140 (MQ=255)                          ttGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTa                                                                                                         <  1:200895/140‑1 (MQ=255)                          ttGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTa                                                                                                         <  1:318223/140‑1 (MQ=255)                            gAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAACTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTaaa                                                                                                       <  1:240803/140‑1 (MQ=255)                                gTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTa                                                                                                         >  2:49592/1‑134 (MQ=255)                                gTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTa                                                                                                         >  2:719169/1‑134 (MQ=255)                                gTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTa                                                                                                         <  1:719169/134‑1 (MQ=255)                                gTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTa                                                                                                         <  1:49592/134‑1 (MQ=255)                                gTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATc                                                                                                   >  2:270104/1‑140 (MQ=255)                                           cgcCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTg                                                                                        >  1:438841/1‑140 (MQ=255)                                            gcCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTgc                                                                                       <  1:270104/140‑1 (MQ=255)                                             ccTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGGAAATATCGTAAATGTCTgcg                                                                                      >  2:185450/1‑140 (MQ=255)                                                      cGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGt                                                                             >  2:35436/1‑140 (MQ=255)                                                             atTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGAc                                                                      <  2:499116/140‑1 (MQ=255)                                                                 aCGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATa                                                                                 <  1:558053/125‑1 (MQ=255)                                                                 aCGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATa                                                                                 >  2:558053/1‑125 (MQ=255)                                                                   gTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATGTCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGa                                                                >  2:63610/1‑140 (MQ=255)                                                                      aTTGACCGGCGGTTTTCCCCCACGGTTTTGCTCATCACCATCGCCACCCGAACCCAGCGCTGCCCGACACCCCTCACCTTTAATAACCTCACCGTAAATATCGTAAATGTCTGCGCCATAACGTTTCTGACCAAAAAAcc                                                             >  2:209938/1‑139 (MQ=255)                                                                        tGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGcc                                                           <  1:35436/140‑1 (MQ=255)                                                                             ggcggTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCa                                                      <  2:220001/140‑1 (MQ=255)                                                                                  tttACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATGTCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGAt                                                 <  1:63610/140‑1 (MQ=255)                                                                                  tttACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGAt                                                 <  1:209938/140‑1 (MQ=255)                                                                                            cGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGc                                            <  1:386308/135‑1 (MQ=255)                                                                                            cGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGc                                            >  2:386308/1‑135 (MQ=255)                                                                                            cGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGAc                                       >  1:336869/1‑140 (MQ=255)                                                                                            cGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGAc                                       >  1:557928/1‑140 (MQ=255)                                                                                            cGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGAc                                       >  1:542383/1‑140 (MQ=255)                                                                                            cGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGAc                                       >  1:769585/1‑140 (MQ=255)                                                                                               ttCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTa                                    <  1:185374/140‑1 (MQ=255)                                                                                                tCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAg                                   >  1:119969/1‑140 (MQ=255)                                                                                                 cAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGt                                  <  2:557928/140‑1 (MQ=255)                                                                                                       atcaCCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTaa                            >  2:793530/1‑140 (MQ=255)                                                                                                         caCCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAata                          <  1:793530/140‑1 (MQ=255)                                                                                                         caCCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAata                          >  1:70305/1‑140 (MQ=255)                                                                                                              tCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAg                     >  2:755179/1‑140 (MQ=255)                                                                                                               cGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAGc                    <  2:448021/140‑1 (MQ=255)                                                                                                                    ccGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAGCAcacc               <  1:334094/140‑1 (MQ=255)                                                                                                                     cGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAGCAcacca              >  1:202333/1‑140 (MQ=255)                                                                                                                            cAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAGCACACCACTATCaa       <  2:336869/140‑1 (MQ=255)                                                                                                                            cAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAGCACACCACTATCaa       <  2:769585/140‑1 (MQ=255)                                                                                                                                 cTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAGCACACCACTATCAACGGCa  <  1:755179/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   TGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAGCACACCACTATCAACGGCA  >  NC_000913/2649330‑2649597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑