Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,660,213	T→C	N26S (AAC→AGC)	iscU ←	scaffold protein for iron‑sulfur cluster assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,660,213	0	T	C	100.0%	100.3 / NA	29	N26S (AAC→AGC)	iscU	scaffold protein for iron‑sulfur cluster assembly
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/19);  total (10/19)

TGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTC  >  NC_000913/2660082‑2660344                                                                                                                                    |                                                                                                                                    tGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCtcgtcg                                                                                                                             <  1:104469/140‑1 (MQ=255) tGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCtcgtcg                                                                                                                             <  1:815289/140‑1 (MQ=255)   gAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCgtt                                                                                                                           >  2:154661/1‑140 (MQ=255)          tAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCa                                                                                                                    >  2:568373/1‑140 (MQ=255)            gCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCaaa                                                                                                                  <  1:707761/140‑1 (MQ=255)                 aCCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAAc                                                                                                             <  2:293993/140‑1 (MQ=255)                 aCCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAAc                                                                                                             <  1:72820/140‑1 (MQ=255)                             aGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAgg                                                                                                                <  1:9660/125‑1 (MQ=255)                             aGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAgg                                                                                                                >  2:9660/1‑125 (MQ=255)                             aGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTAcgcg                                                                                                 <  1:179928/140‑1 (MQ=255)                                                        aCCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTGATGGTCGATAACttttt                                                                      <  1:568373/140‑1 (MQ=255)                                                        aCCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACttttt                                                                      >  2:780727/1‑140 (MQ=255)                                                                  ttGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGc                                                            >  2:534662/1‑140 (MQ=255)                                                                  ttGACTTTAATCTGCAACTTCATCACCTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGc                                                            <  1:580373/140‑1 (MQ=255)                                                                   tGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATTGTCGATAACTTTTTCGCTGTAAGcc                                                           >  2:460159/1‑140 (MQ=255)                                                                     aCTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCAt                                                         >  2:638727/1‑140 (MQ=255)                                                                      cTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCAtt                                                        <  1:252629/140‑1 (MQ=255)                                                                      cTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCAtt                                                        <  1:154661/140‑1 (MQ=255)                                                                                cAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATtctc                                              >  2:9757/1‑140 (MQ=255)                                                                                          aCGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATTGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGa                                    <  1:460159/140‑1 (MQ=255)                                                                                            gTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATa                                  <  1:780727/140‑1 (MQ=255)                                                                                            gTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATa                                  <  1:714323/140‑1 (MQ=255)                                                                                            gTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATa                                  <  1:534662/140‑1 (MQ=255)                                                                                            gTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATa                                  <  1:193001/140‑1 (MQ=255)                                                                                                                   ccaTGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCg           <  1:638727/140‑1 (MQ=255)                                                                                                                      tGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATg        <  1:472127/140‑1 (MQ=255)                                                                                                                       gCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGa          <  1:742536/137‑1 (MQ=255)                                                                                                                       gCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGa          >  2:742536/1‑137 (MQ=255)                                                                                                                            tGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTc  >  2:391156/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                    TGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTC  >  NC_000913/2660082‑2660344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑