Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,698,057	A→G	V165A (GTC→GCC)	pgpC ←	phosphatidylglycerophosphatase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,698,057	0	A	G	100.0%	145.2 / NA	42	V165A (GTC→GCC)	pgpC	phosphatidylglycerophosphatase C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/22);  total (20/22)

CAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGACCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGCAGCCAGGGCGTATCGAAATAAACCGCTTCAACCAG  >  NC_000913/2697921‑2698178                                                                                                                                         |                                                                                                                          cAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCt                                                                                                                        >  1:405573/1‑140 (MQ=255)   gTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCttt                                                                                                                      >  2:761556/1‑140 (MQ=255)   gTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCttt                                                                                                                      >  1:279504/1‑140 (MQ=255)     tGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTc                                                                                                                    <  1:94425/140‑1 (MQ=255)     tGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTc                                                                                                                    >  1:516250/1‑140 (MQ=255)        tGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATg                                                                                                                 <  1:761556/140‑1 (MQ=255)               cACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGa                                                                                                          >  2:484892/1‑140 (MQ=255)                aCCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGAc                                                                                                         <  2:100951/140‑1 (MQ=255)                          gTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGt                                                                                               <  2:405573/140‑1 (MQ=255)                            tACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCa                                                                                             >  2:817328/1‑140 (MQ=255)                            tACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCa                                                                                             >  2:461817/1‑140 (MQ=255)                             aCTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCaa                                                                                            <  2:516250/140‑1 (MQ=255)                              cTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAAt                                                                                           <  1:819366/140‑1 (MQ=255)                                cGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATAc                                                                                         <  1:620934/140‑1 (MQ=255)                                   cAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCa                                                                                      <  1:335623/140‑1 (MQ=255)                                    aaCGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCaa                                                                                     <  1:282909/140‑1 (MQ=255)                                    aaCGATGCTGACAGAAATAAAGCAGCGGATTGTACTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCaa                                                                                     <  2:128403/140‑1 (MQ=255)                                     aCGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCaa                                                                                            <  1:184770/132‑1 (MQ=255)                                     aCGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCaa                                                                                            >  2:184770/1‑132 (MQ=255)                                     aCGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAac                                                                                    >  1:529131/1‑140 (MQ=255)                                             gACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAAt                                                                                           <  1:312818/125‑1 (MQ=255)                                             gACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAAt                                                                                           >  2:312818/1‑125 (MQ=255)                                                  aaaTAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACg                                                                       <  2:272456/140‑1 (MQ=255)                                                             cGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTtggc                                                            >  1:685112/1‑140 (MQ=255)                                                             cGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTtggc                                                            >  2:461156/1‑140 (MQ=255)                                                              ggATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTtggct                                                           <  1:140572/140‑1 (MQ=255)                                                                   gTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGa                                                      <  1:817328/140‑1 (MQ=255)                                                                   gTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGa                                                      <  1:461817/140‑1 (MQ=255)                                                                              tGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTtggc                                                            <  2:401234/123‑1 (MQ=255)                                                                              tGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTtggc                                                            >  1:401234/1‑123 (MQ=255)                                                                              tGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAAcc                                           >  2:410554/1‑140 (MQ=255)                                                                                  gCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCcgcg                                       <  2:685112/140‑1 (MQ=255)                                                                                   cTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCgg                                      >  1:784737/1‑140 (MQ=255)                                                                                             tGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCgg                                      <  1:365458/130‑1 (MQ=255)                                                                                             tGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCgg                                      >  2:365458/1‑130 (MQ=255)                                                                                                 cAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGCAGCCAggg                             >  2:525672/1‑135 (MQ=255)                                                                                                 cAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGCAGCCAggg                             <  1:525672/135‑1 (MQ=255)                                                                                                  aGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGTAGCCAGGGCGTATc                       >  2:167244/1‑140 (MQ=255)                                                                                                   gCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGCAGCCAGGGCGTATCg                      >  2:57225/1‑140 (MQ=255)                                                                                                         gCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGCAGCCAggg                             >  1:793387/1‑127 (MQ=255)                                                                                                         gCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGCAGCCAggg                             <  2:793387/127‑1 (MQ=255)                                                                                                                       ttGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGCAGCCAGGGCGTATCGAAATAAACCGCTTCAACCAg  <  1:376531/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                          CAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGACCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGCAGCCAGGGCGTATCGAAATAAACCGCTTCAACCAG  >  NC_000913/2697921‑2698178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑