Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,699,413	G→A	A219T (GCC→ACC)	yfhH →	putative DNA‑binding transcriptional regulator YfhH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,699,413	0	G	A	100.0%	197.8 / NA	59	A219T (GCC→ACC)	yfhH	putative DNA‑binding transcriptional regulator YfhH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/28);  total (31/28)

CAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCT  >  NC_000913/2699281‑2699549                                                                                                                                     |                                                                                                                                         cAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCa                                                                                                                                   >  2:375724/1‑140 (MQ=255)    gCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACa                                                                                                                                <  2:485629/140‑1 (MQ=255)      gtcgtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGc                                                                                                                              >  2:329001/1‑140 (MQ=255)       tcgtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCg                                                                                                                             >  2:669840/1‑140 (MQ=255)         gtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTg                                                                                                                                     <  1:530920/130‑1 (MQ=255)         gtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTg                                                                                                                                     >  2:530920/1‑130 (MQ=255)         gtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTg                                                                                                                           >  1:486776/1‑140 (MQ=255)          tcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGc                                                                                                                          <  2:244585/140‑1 (MQ=255)            cccTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCtt                                                                                                                        <  1:560488/140‑1 (MQ=255)                    aTCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGc                                                                                                                <  1:709616/140‑1 (MQ=255)                    aTCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGc                                                                                                                <  1:360661/140‑1 (MQ=255)                    aTCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGc                                                                                                                <  1:329001/140‑1 (MQ=255)                     tCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGcc                                                                                                               >  2:431037/1‑140 (MQ=255)                              aGCCATTTGCTACACCGGTGTTCGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTTCCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCa                                                                                                      <  1:606154/140‑1 (MQ=255)                              aGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGTGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCAtt                                                                                                                  <  1:162379/128‑1 (MQ=255)                              aGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGTGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCAtt                                                                                                                  >  2:162379/1‑128 (MQ=255)                               gCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCAt                                                                                                     >  2:787412/1‑140 (MQ=255)                                   tttCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGcc                                                                                                 >  1:385080/1‑140 (MQ=255)                                   tttCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGcc                                                                                                 >  2:689047/1‑140 (MQ=255)                                          cacCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAAc                                                                                          >  2:227769/1‑140 (MQ=255)                                          cacCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAAc                                                                                          <  1:664753/140‑1 (MQ=255)                                              ggTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAAc                                                                                          >  1:824880/1‑136 (MQ=255)                                              ggTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAAc                                                                                          <  2:824880/136‑1 (MQ=255)                                               gtgtACGACGCGAGTTAAACCTGGCGGCAGATGAGCTGCTGCGAGTGGGCGGAAAAGTGCTGGAGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGCGCTTCTCATTGCCTGTATAGCATTGCCGAAGAACAAGCg                                                                                     >  2:104808/1‑140 (MQ=255)                                               gtgtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCg                                                                                     >  1:652551/1‑140 (MQ=255)                                                tgtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGa                                                                                    >  2:704456/1‑140 (MQ=255)                                                tgtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGa                                                                                    >  1:468695/1‑140 (MQ=255)                                                    cgacgCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACaaa                                                                                <  1:131039/140‑1 (MQ=255)                                                    cgacgCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACaaa                                                                                <  1:704456/140‑1 (MQ=255)                                                    cgacgCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACaaa                                                                                <  1:433032/140‑1 (MQ=255)                                                       cgcgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAAc                                                                                          <  1:284726/127‑1 (MQ=255)                                                       cgcgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAAc                                                                                          >  2:284726/1‑127 (MQ=255)                                                       cgcgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACaa                                                                                        <  2:686033/129‑1 (MQ=255)                                                       cgcgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACaa                                                                                        >  1:686033/1‑129 (MQ=255)                                                       cgcgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAg                                                                             >  2:420473/1‑140 (MQ=255)                                                        gcgAGTTAAACCTGGTGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGt                                                                            <  1:570978/140‑1 (MQ=255)                                                         cgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACa                                                                              >  1:686770/1‑137 (MQ=255)                                                         cgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACa                                                                              <  2:686770/137‑1 (MQ=255)                                                          gAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGt                                                                            >  2:631309/1‑138 (MQ=255)                                                          gAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGt                                                                            <  1:631309/138‑1 (MQ=255)                                                           aGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCt                                                                         >  1:373050/1‑140 (MQ=255)                                                            gTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCtt                                                                        >  2:286482/1‑140 (MQ=255)                                                                                agatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCt                                                    <  2:404723/140‑1 (MQ=255)                                                                                  atgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCa                                                  <  1:669840/140‑1 (MQ=255)                                                                                      tgcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGa                                              <  1:431037/140‑1 (MQ=255)                                                                                       gcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGaa                                             >  2:507968/1‑140 (MQ=255)                                                                                          aGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGt                                          >  2:327797/1‑140 (MQ=255)                                                                                            tGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTa                                        >  1:750878/1‑140 (MQ=255)                                                                                             gggCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTaa                                       <  2:160299/140‑1 (MQ=255)                                                                                                cGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACgg                                    >  1:825765/1‑140 (MQ=255)                                                                                                cGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACgg                                    <  2:750878/140‑1 (MQ=255)                                                                                                  gAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGAt                                  >  1:584230/1‑140 (MQ=255)                                                                                                     aaGTGCTGGCGATTACCGGCTTTACTCCAAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTg                               <  1:420473/140‑1 (MQ=255)                                                                                                                         tttACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTca           >  2:587356/1‑140 (MQ=255)                                                                                                                         tttACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTca           >  2:424415/1‑140 (MQ=255)                                                                                                                            aCTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTcagca        <  1:507968/140‑1 (MQ=255)                                                                                                                            aCTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTcagca        <  2:486776/140‑1 (MQ=255)                                                                                                                                 gAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATc   >  2:611458/1‑140 (MQ=255)                                                                                                                                  aaTACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCt  >  2:658931/1‑140 (MQ=255)                                                                                                                                  aaTACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCt  <  1:375724/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         CAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCT  >  NC_000913/2699281‑2699549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑