Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,707,964	T→C	N264S (AAC→AGC)	rseB ←	anti‑sigma factor stabilizing protein RseB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,707,964	0	T	C	100.0%	102.0 / NA	30	N264S (AAC→AGC)	rseB	anti‑sigma factor stabilizing protein RseB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/17);  total (13/17)

AATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAAT  >  NC_000913/2707831‑2708098                                                                                                                                      |                                                                                                                                       aaTGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCg                                                                                                                                  <  2:573469/140‑1 (MQ=255)   tGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGTCGTAGCGCGGTTAACGCTTACCgag                                                                                                                                <  1:514452/140‑1 (MQ=255)          cGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCgaga                                                                                                                               >  2:654615/1‑134 (MQ=255)          cGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCgaga                                                                                                                               <  1:654615/134‑1 (MQ=255)              gTTTGCGGCGGCTGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCCATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGaataa                                                                                                                     <  1:223274/140‑1 (MQ=255)                   cggcggCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGt                                                                                                                <  1:418623/140‑1 (MQ=255)                     gcggcAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCg                                                                                                              <  2:602677/140‑1 (MQ=255)                                   aCAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGa                                                                                                <  2:369478/140‑1 (MQ=255)                                    cAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGAt                                                                                               <  2:773055/140‑1 (MQ=255)                                     aaTGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGAtt                                                                                              <  2:442840/140‑1 (MQ=255)                                      aTGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGAtt                                                                                              <  2:601234/139‑1 (MQ=255)                                      aTGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGAtt                                                                                              >  1:601234/1‑139 (MQ=255)                                              ttCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCa                                                                                     >  1:828824/1‑140 (MQ=255)                                                 ggCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCAtgt                                                                                  >  1:15416/1‑140 (MQ=255)                                                    gTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCAtgttgt                                                                               <  2:397760/140‑1 (MQ=255)                                                                  ttGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCAtgttgt                                                                               >  1:773326/1‑126 (MQ=255)                                                                  ttGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCAtgttgt                                                                               <  2:773326/126‑1 (MQ=255)                                                                  ttGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGc                                                                 >  1:522237/1‑140 (MQ=255)                                                                  ttGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGc                                                                 >  1:332182/1‑140 (MQ=255)                                                                    gTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCgg                                                               >  1:771169/1‑140 (MQ=255)                                                                                      ccGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAAc                                             <  2:332182/140‑1 (MQ=255)                                                                                        ggTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACtt                                           <  2:522237/140‑1 (MQ=255)                                                                                        ggTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACtt                                           <  2:771169/140‑1 (MQ=255)                                                                                          tGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCg                                         >  1:591509/1‑140 (MQ=255)                                                                                                                      cGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTg                >  2:562386/1‑137 (MQ=255)                                                                                                                      cGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTg                <  1:562386/137‑1 (MQ=255)                                                                                                                           cgcgGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAg        >  1:617687/1‑140 (MQ=255)                                                                                                                             cgGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCt      >  1:811/1‑140 (MQ=255)                                                                                                                             cgGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCt      >  1:501728/1‑140 (MQ=255)                                                                                                                                 tAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAAt  <  2:811/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       AATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAAT  >  NC_000913/2707831‑2708098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑