Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,734,706	A→G	V110A (GTG→GCG)	pgeF ←	polyphenol oxidase YfiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,734,706	0	A	G	100.0%	127.9 / NA	39	V110A (GTG→GCG)	pgeF	polyphenol oxidase YfiH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/18);  total (21/18)

GGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGA  >  NC_000913/2734571‑2734841                                                                                                                                        |                                                                                                                                        ggCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAgg                                                                                                                                     >  2:131476/1‑140 (MQ=255) ggCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAgg                                                                                                                                     >  2:68500/1‑140 (MQ=255)   cccTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGcgca                                                                                                                                       >  2:746491/1‑136 (MQ=255)         ccAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAggcagt                                                                                                                             >  1:232228/1‑140 (MQ=255)             gCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAggcagtcggc                                                                                                                         >  2:696618/1‑140 (MQ=255)                                tATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAAcc                                                                                                      >  2:177239/1‑140 (MQ=255)                                         aaCAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGc                                                                                             >  2:544456/1‑140 (MQ=255)                                          aCAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCg                                                                                            <  1:696618/140‑1 (MQ=255)                                            aGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGt                                                                                           >  1:182836/1‑139 (MQ=255)                                            aGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGt                                                                                           <  2:182836/139‑1 (MQ=255)                                                aaCCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGt                                                                                           <  2:640717/135‑1 (MQ=255)                                                aaCCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGt                                                                                           >  1:640717/1‑135 (MQ=255)                                                   cGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCtat                                                                                   >  2:310597/1‑140 (MQ=255)                                                     tctcTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCCCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATaa                                                                                 >  2:154148/1‑140 (MQ=255)                                                      ctctTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAag                                                                                >  1:47731/1‑140 (MQ=255)                                                      ctctTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAag                                                                                >  2:522705/1‑140 (MQ=255)                                                             aGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATc                                                                         >  1:605253/1‑140 (MQ=255)                                                                   ccTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGTGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCg                                                                   >  2:764809/1‑140 (MQ=255)                                                                   ccTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCg                                                                   >  2:711216/1‑140 (MQ=255)                                                                       cgcACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGtttt                                                               <  1:154148/140‑1 (MQ=255)                                                                       cgcACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGtttt                                                               <  2:519433/140‑1 (MQ=255)                                                                       cgcACAGTCCACGCCAGCAAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGtttt                                                               <  2:129666/140‑1 (MQ=255)                                                                              tCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCAt                                                        <  2:232228/140‑1 (MQ=255)                                                                                 aCGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAg                                                     <  1:544456/140‑1 (MQ=255)                                                                                      agccagcATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCg                                                >  2:179754/1‑140 (MQ=255)                                                                                       gccagcATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGc                                               <  1:764809/140‑1 (MQ=255)                                                                                        ccagcATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGcc                                              >  2:13068/1‑140 (MQ=255)                                                                                        ccagcATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGcc                                              >  2:620210/1‑140 (MQ=255)                                                                                               gAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGc                                       <  1:734596/140‑1 (MQ=255)                                                                                                 gcggcggcgACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCtt                                     <  1:711216/140‑1 (MQ=255)                                                                                                    gcggcgACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAg                                  <  1:522705/140‑1 (MQ=255)                                                                                                      ggcgACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGca                                >  2:743784/1‑140 (MQ=255)                                                                                                       gcgACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGcac                               >  2:453572/1‑140 (MQ=255)                                                                                                            ttCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCtt                          <  1:131476/140‑1 (MQ=255)                                                                                                              ccGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTg                        <  1:177239/140‑1 (MQ=255)                                                                                                                 ttCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCg                     <  1:620210/140‑1 (MQ=255)                                                                                                                           aTTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCt           <  1:13068/140‑1 (MQ=255)                                                                                                                                    cAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCt           >  2:309818/1‑131 (MQ=255)                                                                                                                                    cAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCt           <  1:309818/131‑1 (MQ=255)                                                                                                                                    cAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGa  <  1:453572/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        GGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGA  >  NC_000913/2734571‑2734841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑