Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,861,516	T→C	G29G (GGT→GGC)	ygbJ →	putative L‑threonate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,861,516	0	T	C	100.0%	200.6 / NA	57	G29G (GGT→GGC)	ygbJ	putative L‑threonate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/25);  total (32/25)

TATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGAAAAACTGGACGCACTGCTGGTGCTGGTGGTCAATGCG  >  NC_000913/2861380‑2861651                                                                                                                                         |                                                                                                                                        tataTTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCtt                                                                                                                                      <  2:217526/140‑1 (MQ=255)  atatTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCttt                                                                                                                                     >  1:636191/1‑140 (MQ=255)          aaTATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCt                                                                                                                               >  1:50401/1‑138 (MQ=255)          aaTATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCt                                                                                                                               <  2:50401/138‑1 (MQ=255)          aaTATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTgg                                                                                                                             >  1:798515/1‑140 (MQ=255)                 aacaaaATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGCCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAg                                                                                                                      >  2:138643/1‑140 (MQ=255)                       atatCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGa                                                                                                                <  1:776127/140‑1 (MQ=255)                       atatCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGa                                                                                                                <  1:732514/140‑1 (MQ=255)                       atatCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGa                                                                                                                <  1:498500/140‑1 (MQ=255)                        tatCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGaa                                                                                                               >  2:608906/1‑140 (MQ=255)                         atCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAAc                                                                                                              <  1:779630/140‑1 (MQ=255)                                 aaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGc                                                                                                      <  2:636191/140‑1 (MQ=255)                                 aaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGc                                                                                                      <  1:608906/140‑1 (MQ=255)                                 aaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGc                                                                                                      <  1:670973/140‑1 (MQ=255)                                       cAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTgcgc                                                                                                <  2:410265/140‑1 (MQ=255)                                           gagaGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACg                                                                                            <  1:91038/140‑1 (MQ=255)                                            agagCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGt                                                                                           >  2:691193/1‑140 (MQ=255)                                            agagCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGt                                                                                           >  2:763326/1‑140 (MQ=255)                                              agCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTg                                                                                         <  2:798515/140‑1 (MQ=255)                                                cAGATGAAAACGGGGTCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGaa                                                                                       >  1:677111/1‑140 (MQ=255)                                                cAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTgcg                                                                                                 <  2:699482/130‑1 (MQ=255)                                                cAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTgcg                                                                                                 >  1:699482/1‑130 (MQ=255)                                                cAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGaa                                                                                       <  1:763326/140‑1 (MQ=255)                                                cAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGaa                                                                                       >  1:184306/1‑140 (MQ=255)                                                 aGATGAAAACGGGATCTGAGTTTCATCTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGaaa                                                                                      <  1:580775/140‑1 (MQ=255)                                                  gATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGaa                                                                                       >  1:826847/1‑138 (MQ=255)                                                  gATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGaa                                                                                       <  2:826847/138‑1 (MQ=255)                                                    tGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAagag                                                                                   >  1:453515/1‑140 (MQ=255)                                                         aCGGGGTCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAgg                                                                              <  2:677111/140‑1 (MQ=255)                                                             gATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCt                                                                          >  1:495255/1‑140 (MQ=255)                                                             gATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCt                                                                          >  1:218436/1‑140 (MQ=255)                                                             gATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCt                                                                          >  2:15751/1‑140 (MQ=255)                                                                cTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCTGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGc                                                                       >  2:815523/1‑140 (MQ=255)                                                                 tGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCg                                                                      <  1:691193/140‑1 (MQ=255)                                                                       tCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGttt                                                                >  1:765801/1‑140 (MQ=255)                                                                           gTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCg                                                                      <  1:75682/130‑1 (MQ=255)                                                                           gTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCg                                                                      >  2:75682/1‑130 (MQ=255)                                                                                       ggCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGAc                                                >  2:253036/1‑140 (MQ=255)                                                                                          ttAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTACGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGtt                                             >  2:711516/1‑140 (MQ=255)                                                                                            aGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTg                                           <  1:532955/140‑1 (MQ=255)                                                                                               gTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCg                                        <  2:218436/140‑1 (MQ=255)                                                                                                  aatgggaatgggAGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGaaa                                     >  1:302018/1‑140 (MQ=255)                                                                                                            ggaGCAGCACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGAAAAACTGGACGc                           <  2:165709/140‑1 (MQ=255)                                                                                                              agcagcACTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGAAAAACTGGACGCAc                         >  2:516720/1‑140 (MQ=255)                                                                                                                cagcaCTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGAAAAACTGGACGCActg                       >  1:313428/1‑140 (MQ=255)                                                                                                                  gcaCTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGAAAAACTGGACGCActgct                     >  1:564445/1‑140 (MQ=255)                                                                                                                  gcaCTGTCATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGAAAAACTGGACGCActgct                     <  2:765801/140‑1 (MQ=255)                                                                                                                         cATATGTCCGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGAAAAACTGGACGCACtgctggtgctg              <  2:133557/140‑1 (MQ=255)                                                                                                                                ccGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGAAAAACTGGACGCACTGCTGGTGCTGGTGGTCa       >  1:811903/1‑140 (MQ=255)                                                                                                                                ccGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGAAAAACTGGACGCACTGCTGGTGCTGGTGGTCa       >  1:522951/1‑140 (MQ=255)                                                                                                                                ccGCGCAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGAAAAACTGGACGCACTGCTGGTGCTGGTGGTCa       >  1:227630/1‑140 (MQ=255)                                                                                                                                  gcgcAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGAAAAACTGGACGCACTGCTGGTGCTGGTGGTCAAt     >  1:386993/1‑140 (MQ=255)                                                                                                                                  gcgcAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGAAAAACTGGACGCACTGCTGGTGCTGGTGGTCAAt     >  1:52598/1‑140 (MQ=255)                                                                                                                                  gcgcAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGAAAAACTGGACGCACTGCTGGTGCTGGTGGTCAAt     >  2:301917/1‑140 (MQ=255)                                                                                                                                   cgcAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGAAAAACTGGACGCACTGCTGGTGCTGGTGGTCAATg    <  1:301917/140‑1 (MQ=255)                                                                                                                                   cgcAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGAAAAACCGGACGCACTGCTGGTGCTGGTGGTCAATg    >  1:634464/1‑140 (MQ=255)                                                                                                                                    gcAGGCCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCG‑AAAACTGGACGCACTGCTGGTGCTGGTGGTCAATGCg  >  1:422156/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTTCTGATAACGCCGCGACGTTTGCCGAAAAACTGGACGCACTGCTGGTGCTGGTGGTCAATGCG  >  NC_000913/2861380‑2861651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑