Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,946,652	T→C	N176S (AAC→AGC)	mltA ←	membrane‑bound lytic murein transglycosylase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,946,652	0	T	C	100.0%	132.5 / NA	38	N176S (AAC→AGC)	mltA	membrane‑bound lytic murein transglycosylase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/18);  total (20/18)

TCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGTTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCC  >  NC_000913/2946520‑2946780                                                                                                                                     |                                                                                                                                 tCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCAt                                                                                                                           <  2:546078/140‑1 (MQ=255)     cACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAgg                                                                                                                       <  2:596468/140‑1 (MQ=255)             aTCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACt                                                                                                               >  1:29822/1‑140 (MQ=255)              tCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTg                                                                                                              <  1:65483/140‑1 (MQ=255)                    ccTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCg                                                                                                        >  2:645558/1‑140 (MQ=255)                       tACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTg                                                                                                              <  2:739561/131‑1 (MQ=255)                       tACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTg                                                                                                              >  1:739561/1‑131 (MQ=255)                         ccAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAat                                                                                                   <  1:613722/140‑1 (MQ=255)                           aaTGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAatat                                                                                                 <  2:612211/140‑1 (MQ=255)                            aTGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAatata                                                                                                >  1:658801/1‑140 (MQ=255)                                 gcGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTAt                                                                                           <  2:658801/140‑1 (MQ=255)                                  cGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGTCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATc                                                                                          >  1:292563/1‑140 (MQ=255)                                   gATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCa                                                                                         <  1:714857/140‑1 (MQ=255)                                       ggCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCa                                                                                     >  2:66899/1‑140 (MQ=255)                                                            aTAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCg                                                                >  1:118528/1‑140 (MQ=255)                                                                cTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACg                                                            >  2:113989/1‑140 (MQ=255)                                                                   aaaaaGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACgaga                                                         >  2:453198/1‑140 (MQ=255)                                                                                 aCTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCAc                                           >  1:313583/1‑140 (MQ=255)                                                                                  cTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACg                                          >  1:787868/1‑140 (MQ=255)                                                                                     ccATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGttt                                       <  2:118528/140‑1 (MQ=255)                                                                                      cATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGtttt                                      >  2:721358/1‑140 (MQ=255)                                                                                        tCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTgg                                    >  2:128217/1‑140 (MQ=255)                                                                                              aaGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCAt                              <  1:693849/140‑1 (MQ=255)                                                                                              aaGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCAt                              <  1:453198/140‑1 (MQ=255)                                                                                               aGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATa                             <  2:787868/140‑1 (MQ=255)                                                                                               aGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATa                             <  1:128217/140‑1 (MQ=255)                                                                                                 tCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACg                           <  1:296277/140‑1 (MQ=255)                                                                                                    atatACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTgg                                    <  1:73550/128‑1 (MQ=255)                                                                                                    atatACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTgg                                    >  2:73550/1‑128 (MQ=255)                                                                                                          ccACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAgg                  <  1:721358/140‑1 (MQ=255)                                                                                                                   tGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGt                         <  2:470537/124‑1 (MQ=255)                                                                                                                   tGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGt                         >  1:470537/1‑124 (MQ=255)                                                                                                                   tGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGat         >  2:328888/1‑139 (MQ=255)                                                                                                                   tGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGaa         >  2:58150/1‑140 (MQ=255)                                                                                                                   tGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGaa         >  1:729314/1‑140 (MQ=255)                                                                                                                        aTCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGc    >  2:71072/1‑140 (MQ=255)                                                                                                                         tCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGcc   <  2:29822/140‑1 (MQ=255)                                                                                                                          ccATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGccc  >  2:153890/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                 TCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGTTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCC  >  NC_000913/2946520‑2946780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑